中华航海医学与高气压医学杂志
中華航海醫學與高氣壓醫學雜誌
중화항해의학여고기압의학잡지
CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
2008年
4期
200-203
,共4页
沈先荣%栾洁%蒋定文%陈伟%刘李娜%陆敏%贾福星
瀋先榮%欒潔%蔣定文%陳偉%劉李娜%陸敏%賈福星
침선영%란길%장정문%진위%류리나%륙민%가복성
棘刺锚参%肝癌%肺腺癌%癌肉瘤%肿瘤治疗
棘刺錨參%肝癌%肺腺癌%癌肉瘤%腫瘤治療
극자묘삼%간암%폐선암%암육류%종류치료
Protankyra Bidentata%Lung cancer%Liver cancer%Sarcoma%Tumor therapy
目的 研究棘刺锚参抗肿瘤因子(Protankyra Bidentata anti-tumor factor,PBATF)在动物水平的抗肿瘤作用.方法 采用小鼠H22肝癌模型、大鼠Walker-256癌肉瘤模型、人体LAX肺腺癌模型、人体QGY肝癌模型,分别测定PBATF对不同肿瘤生长的抑制效应.各种模型分别设PBATF高(5 mg/kg)、中(1 mg/ks)、低(0.5 mg/kg)剂量组、阳性对照组(CTX)和阴性对照组.试验组及阳性对照组昆明种小鼠或Wiser大鼠每组均10只,裸小鼠每组均6只,阴性对照组每次各为2组.腹腔注射给药,每天1次,连续7～10 d.采用常规方法测定PBATF腹腔注射的LD50.结果 PBATF对大鼠Walker-256癌肉瘤的抑瘤率,高剂量组62.63%、中剂量组52.31%、低剂量37.49%.PBATF对人体LAX肺腺癌的抑瘤率,高剂量组56.02%、中剂量组50.60%、低剂量组40.36%.PBATF对人体QGY肝癌的抑瘤率,高剂量组52.07%、中剂量组45.56%、低剂量组33.14%.PBATF对小鼠H22肝癌的抑瘤率,高剂量组59.25%、中剂量组57.74%、低剂量组45.28%.PBATF腹腔注射给药,小鼠的LD50为1.973 g/kg.结论 获得了高纯度的PBATF,能够显著抑制体内外肿瘤生长.
目的 研究棘刺錨參抗腫瘤因子(Protankyra Bidentata anti-tumor factor,PBATF)在動物水平的抗腫瘤作用.方法 採用小鼠H22肝癌模型、大鼠Walker-256癌肉瘤模型、人體LAX肺腺癌模型、人體QGY肝癌模型,分彆測定PBATF對不同腫瘤生長的抑製效應.各種模型分彆設PBATF高(5 mg/kg)、中(1 mg/ks)、低(0.5 mg/kg)劑量組、暘性對照組(CTX)和陰性對照組.試驗組及暘性對照組昆明種小鼠或Wiser大鼠每組均10隻,裸小鼠每組均6隻,陰性對照組每次各為2組.腹腔註射給藥,每天1次,連續7～10 d.採用常規方法測定PBATF腹腔註射的LD50.結果 PBATF對大鼠Walker-256癌肉瘤的抑瘤率,高劑量組62.63%、中劑量組52.31%、低劑量37.49%.PBATF對人體LAX肺腺癌的抑瘤率,高劑量組56.02%、中劑量組50.60%、低劑量組40.36%.PBATF對人體QGY肝癌的抑瘤率,高劑量組52.07%、中劑量組45.56%、低劑量組33.14%.PBATF對小鼠H22肝癌的抑瘤率,高劑量組59.25%、中劑量組57.74%、低劑量組45.28%.PBATF腹腔註射給藥,小鼠的LD50為1.973 g/kg.結論 穫得瞭高純度的PBATF,能夠顯著抑製體內外腫瘤生長.
목적 연구극자묘삼항종류인자(Protankyra Bidentata anti-tumor factor,PBATF)재동물수평적항종류작용.방법 채용소서H22간암모형、대서Walker-256암육류모형、인체LAX폐선암모형、인체QGY간암모형,분별측정PBATF대불동종류생장적억제효응.각충모형분별설PBATF고(5 mg/kg)、중(1 mg/ks)、저(0.5 mg/kg)제량조、양성대조조(CTX)화음성대조조.시험조급양성대조조곤명충소서혹Wiser대서매조균10지,라소서매조균6지,음성대조조매차각위2조.복강주사급약,매천1차,련속7～10 d.채용상규방법측정PBATF복강주사적LD50.결과 PBATF대대서Walker-256암육류적억류솔,고제량조62.63%、중제량조52.31%、저제량37.49%.PBATF대인체LAX폐선암적억류솔,고제량조56.02%、중제량조50.60%、저제량조40.36%.PBATF대인체QGY간암적억류솔,고제량조52.07%、중제량조45.56%、저제량조33.14%.PBATF대소서H22간암적억류솔,고제량조59.25%、중제량조57.74%、저제량조45.28%.PBATF복강주사급약,소서적LD50위1.973 g/kg.결론 획득료고순도적PBATF,능구현저억제체내외종류생장.
Objective To study the inhibitory effects of Protankyra Bidentata anti-tumor factor(PBATF)on the growth of tumors implanted in mice or rats.Methods Methods of H22 liver cancer implanted in Kunming mice,Walker-256 sarcoma implanted in Wistar rats,human LAX lung cancer and human QGY liver cancer implanted in nest mice were used to detect the inhibitory effects of PBATF on the tumor growth,respectively.Animals in each model were divided into 5 groups:hish PBATF group(5 mg/kg),middle PBATF group(1 mg/kg),low PBATF group(0.5 mg/kg),positive control group(CTX)and negative control group.The preparations were administrated once a day,for 7-10 days.The LD50 peritoneal injection of PBATF was detected according to ordinary method.Results The inhibitory tumor rates of PBATF on Walker-256 sarcoma implanted in Wistar rats,human LAX lung caner implanted in nest mice,human QGY liver cancer implanted in nest mice and QGY liver cancer implanted in nest mice were 62.63%,56.02%,52.07% and 59.25% in high dose group,52.31%,50.60%,45.56% and 57.74% in middle dose group,37.49%,40.36%,33.14% and 45.28% in low dose group,respectively.The LD50 peritoneal injection of PBATF was 1.973 g/kg.Conclusions PBATF hag significant inhibitory effect on tumor growth in vivo.
