CAJ | 학술논문

目的研究海风藤提取物对纤丝状和寡聚体β淀粉样蛋白(β-amyloid,Aβ)诱导的活化新生大鼠小胶质细胞可能的影响.方法原代培养新生大鼠小胶质细胞,分别用纤丝状Aβ25-35、寡聚体Aβ25-35、纤丝状Aβ25-35+海风藤提取物、寡聚体Aβ25-35+海风藤提取物干预小胶质细胞,并设立空白对照组.干预48h后,以CD68作为小胶质细胞活化的特异性抗原标志,利用免疫细胞化学染色方法,对小胶质细胞的活化率进行检测,并比较不同处理组间的差异.结果四个实验组的小胶质细胞CD68表达阳性率均高于空白对照组(5.1％,P＜0.05).纤丝状Aβ+海风藤提取物组CD68表达阳性率(52.1％)低于纤丝状Aβ组(60.8％,P＜0.05).寡聚体Aβ组(71.2％)和寡聚体Aβ+海风藤提取物组(67.0％)的CD68表达阳性率无明显差异(P=0.101).结论纤丝状和寡聚体Aβ均能激活小胶质细胞；海风藤提取物能够抑制纤丝状Aβ对小胶质细胞的激活作用,对寡聚体Aβ的激活作用无明显影响.
목적 연구해풍등제취물대섬사상화과취체β정분양단백(β-amyloid,Aβ)유도적활화신생대서소효질세포가능적영향.방법 원대배양신생대서소효질세포,분별용섬사상Aβ25-35、과취체Aβ25-35、섬사상Aβ25-35+해풍등제취물、과취체Aβ25-35+해풍등제취물간예소효질세포,병설립공백대조조.간예48h후,이CD68작위소효질세포활화적특이성항원표지,이용면역세포화학염색방법,대소효질세포적활화솔진행검측,병비교불동처리조간적차이.결과 사개실험조적소효질세포CD68표체양성솔균고우공백대조조(5.1％,P＜0.05).섬사상Aβ+해풍등제취물조CD68표체양성솔(52.1％)저우섬사상Aβ조(60.8％,P＜0.05).과취체Aβ조(71.2％)화과취체Aβ+해풍등제취물조(67.0％)적CD68표체양성솔무명현차이(P=0.101).결론 섬사상화과취체Aβ균능격활소효질세포；해풍등제취물능구억제섬사상Aβ대소효질세포적격활작용,대과취체Aβ적격활작용무명현영향.
Objective To compare the activation of microglia in vitro induced by oligomeric and fibrillar Aβ,and research the effects of Piper Kadsura Ohwi extracts on the microglial activation.Methods Microglia were divided randomly into 4 test groups,intervened by fibrillar Aβ25-35,fibrillar Aβ25-35 + Piper Kadsura Ohwi extracts,oligomeric Aβ25-35 and oligomeric Aβ25-35 + Piper Kadsura Ohwi extracts respectively.Also a blank contrast group was set without any intervention.48 hours later,activation of microglia was tested by immunocytochemistry,using the CD68 molecule as a specific marker of microglial activation and the incidences of active microglia in different groups were compared.Results Each of the 4 test groups had a higher positive incidence of CD68 expression than that of the blank contrast (5.1％ ) (P ＜ 0.05 ) ; positive incidence of fibrillar Aβ + Piper Kadsura Ohwi extracts group (52.1％) was lower than that of the fibrillar Aβ group (60.8％) (P＜0.05) ; positive incidence of oligomeric Aβ + Piper Kadsura Ohwi extracts group (67.0％) was not significantly different with that of the oligomeric Aβ group (71.2％),P=0.101.Conclusion Both fibrillar and oligomeric Aβ has the ability to activate the silent microglia.Piper Kadsura Ohwi extracts could inhibit the activation of microglia induced by fibrillar Aβ25-35,but didn't show significant effects on the activation induced by oligomeric Aβ25-35.