CAJ | 학술논문

目的探讨自体骨髓单核细胞移植对血管新生及胆道缺血病变的影响.方法将30只日本大耳白兔随机分为3组:假手术组(A组)、实验模型组(B组)和骨髓单核细胞移植组(C组),每组10只.3组均游离胆总管、肝总动脉及其间的疏松结缔组织,B组和C组阻断胆总管远端及肝总动脉2 h,C组经肝总动脉注射PKH26标记的自体骨髓单核细胞.术后监测生化指标的变化情况.术后4周开腹行胆道造影.同时,取第一肝门处汇管区肝组织制作石蜡切片,应用免疫组织化学方法观察自体骨髓单核细胞在肝内缺血环境中的分布及分化情况,并检测微血管密度.结果术后C组生化指标恢复明显优于B组,骨髓单核细胞可分化成血管内皮细胞.术后4周,C组胆道破坏较B组轻,且胆道周围新生毛细血管多于B组.结论自体骨髓单核细胞移植可以促进局部缺血组织毛细血管的增生,改善局部缺血胆道组织的微循环,从而减轻缺血型胆道病变的程度,甚至预防缺血型胆道病变的发生.
목적 탐토자체골수단핵세포이식대혈관신생급담도결혈병변적영향.방법 장30지일본대이백토수궤분위3조:가수술조(A조)、실험모형조(B조)화골수단핵세포이식조(C조),매조10지.3조균유리담총관、간총동맥급기간적소송결체조직,B조화C조조단담총관원단급간총동맥2 h,C조경간총동맥주사PKH26표기적자체골수단핵세포.술후감측생화지표적변화정황.술후4주개복행담도조영.동시,취제일간문처회관구간조직제작석사절편,응용면역조직화학방법관찰자체골수단핵세포재간내결혈배경중적분포급분화정황,병검측미혈관밀도.결과 술후C조생화지표회복명현우우B조,골수단핵세포가분화성혈관내피세포.술후4주,C조담도파배교B조경,차담도주위신생모세혈관다우B조.결론 자체골수단핵세포이식가이촉진국부결혈조직모세혈관적증생,개선국부결혈담도조직적미순배,종이감경결혈형담도병변적정도,심지예방결혈형담도병변적발생.
Objective To investigate the effect of implantation of bone marrow mononuclear cells (BM-MNCs)on neovascularization and ischemic-type intrahepatic biliary lesion in rabbits.Methods The animals were divided into the sham-operation group, experimental model group and BM-MNCs implantation group with 10 rabbits in each. The animal model of ischemic-type intrahepatic biliary lesion was established by clamping the hepatic artery and common bile duct. The BM-MNCswere isolated from the tibial plateau by means of density gradient centrifugation and were implanted through the common hepatic artery. Changes of some biochemical markers such as ALT, AST, ALP,γ-GT, TBIL and DBIL etc. were detected. In 4 weeks after operation, the cholangiography, histopathological manifestation, differentiation of BM-MNCs, and microvessel density were observed.Results At each observation time, the degree of change of biochemical markers in group C was lower than that in group B. The engrafted cells could differentiate into vascular endothelial cells. The intrahepatic biliary lesion of group B was severer than that of group C but had fewer new capillary blood vessels around it. Conclusion The implantation of BM-MNCs can promote neovascularization and increase blood supply to the ischemic bile duct to diminish or prevent ischemic-type intrahepatic biliary lesion.