CAJ | 학술논문

目的通过体外实验,观察利巴韦林注射液对肠道病毒71型(EV71)病毒在横纹肌肉瘤传代细胞(RD-A细胞)复制的抑制作用和体外灭活作用.方法使用在2008年4月安徽阜阳疫情中分离到的EV71病毒,通过三种体外细胞实验:药物在细胞中抑制EV71病毒复制的药效测定实验;药物对细胞的保护作用实验;药物体外对EV71病毒的灭活作用的药效测定实验.观察利巴韦林注射液对EV71病毒感染横纹肌肉瘤传代细胞(RD-A细胞)中所起的作用.结果在利巴韦林注射液在细胞中抑制EV71病毒复制的药效测定实验中,1:640利巴韦林注射液稀释度组较病毒对照组延迟病变出现2 d,细胞生长未受到影响.在药物对细胞的保护作用实验中,1:8利巴韦林稀释度组较病毒对照组延迟病变出现4 d.在利巴韦林注射液药物体外对EV71病毒的灭活作用的药效测定研究中,1:40稀释度组较病毒对照组延迟病变出现2 d,而细胞生长未受到影响.结论利巴韦林注射液在体外有抑制EV71病毒复制和部分灭活病毒的作用;并有一定的保护细胞抑制EV71病毒感染的作用.本研究对于临床上EV71感染的早期预防和治疗具有一定的指导意义.
목적 통과체외실험,관찰리파위림주사액대장도병독71형(EV71)병독재횡문기육류전대세포(RD-A세포)복제적억제작용화체외멸활작용.방법 사용재2008년4월안휘부양역정중분리도적EV71병독,통과삼충체외세포실험:약물재세포중억제EV71병독복제적약효측정실험;약물대세포적보호작용실험;약물체외대EV71병독적멸활작용적약효측정실험.관찰리파위림주사액대EV71병독감염횡문기육류전대세포(RD-A세포)중소기적작용.결과 재리파위림주사액재세포중억제EV71병독복제적약효측정실험중,1:640리파위림주사액희석도조교병독대조조연지병변출현2 d,세포생장미수도영향.재약물대세포적보호작용실험중,1:8리파위림희석도조교병독대조조연지병변출현4 d.재리파위림주사액약물체외대EV71병독적멸활작용적약효측정연구중,1:40희석도조교병독대조조연지병변출현2 d,이세포생장미수도영향.결론 리파위림주사액재체외유억제EV71병독복제화부분멸활병독적작용;병유일정적보호세포억제EV71병독감염적작용.본연구대우림상상EV71감염적조기예방화치료구유일정적지도의의.
Objective Observe the effect of Ribavirin on reducing the EV71 replication, inactivating EV71 and protecting the RD-A cells against the EV71 infection in vitro. Methods Using the EV71 isolated from Anhui Fuyang HFMD outbreak, the effect of Ribavirin on RD-A cells during the EV71 infection was observed. Results In the experiment of Ribavirin inhibiting the EV71 replication, comparing with the no-Ribavirin-dealed virus control group, the group of 1:640 dilution of Ribavirin delayed the CPE for 2 days, while the normal cell group was growing very well. In the experiment of protecting cell from EV71 infection, comparing with the no-Ribavirin-dealed virus control group, the group of 1:8 dilution of Ribavirin delayed the CPE for 4 days. In the experiment of Ribavirin effect on the inactivation of EV71, the group of 1:40 dilution of Ribavirin delayed the CPE for 2 days comparing with the virus control group. Conclusion Ribavirin could inhibit the replication of the EV71 and inactivate the EV71 in vitro. Additionally, Ribavirin could protect RD-A cells from EV 71 infection. The observation will contribute to EV71 infection control and quick medicine therapy.