中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2008年
10期
717-721
,共5页
李筑英%颜崇淮%徐健%黄华%邹向宇
李築英%顏崇淮%徐健%黃華%鄒嚮宇
리축영%안숭회%서건%황화%추향우
妊娠压力%铅%环境暴露%迷宫学习%记忆
妊娠壓力%鉛%環境暴露%迷宮學習%記憶
임신압력%연%배경폭로%미궁학습%기억
Prenatal stress%Lead%Environmental exposure%Maze learning%Memory
目的 探讨妊娠压力与铅联合暴露对大鼠子代早期空间学习记忆能力的影响.方法 运用数字表法随机将32只Sprague-Dawley孕鼠分为空白对照组(NS/C),铅暴露组(NS/L),压力暴露组(S/C),联合暴露组(S/L),每组8只.NS/L、S/L自由饮用0.2%醋酸铅溶液,S/C、S/L给予束缚压力.子代于30 d龄进行Morris水迷宫测试,并检测海马组织铅含量及水迷宫实验前、后血清肾上腺酮.结果 S/L雄、雌性仔鼠在原平台所在象限停留时间分别为(16.08 ±3.41)s、(15.72 ±3.33)s,显著短于NS/L(25.42±4.76)s、(24.55±4.43)s和S/C(20.96±3.45)s、(20.65±2.98)s,妊娠压力与铅对仔鼠在原平台所在象限停留时间的影响存在交互作用(F=5.478,P<0.05);妊娠压力与铅对仔鼠应激后血清肾上腺酮水平的影响存在交互作用.NS/L、S/L仔鼠海马铅含量分别为(0.4378 ±0.1041)μg/g、(0.4679 ±0.1243)μg/g,差异无统计学意义(F=0.298,P0.05).结论 (1)妊娠压力与铅对大鼠子代学习记忆的损害可能具有叠加作用.(2)妊娠压力与铅对仔鼠下丘脑.垂体-肾上腺轴的影响可能具有叠加作用.该作用可能是二者对子代学习记忆叠加损害的原因之一.(3)联合暴露未显著增加铅在子代海马中的蓄积.
目的 探討妊娠壓力與鉛聯閤暴露對大鼠子代早期空間學習記憶能力的影響.方法 運用數字錶法隨機將32隻Sprague-Dawley孕鼠分為空白對照組(NS/C),鉛暴露組(NS/L),壓力暴露組(S/C),聯閤暴露組(S/L),每組8隻.NS/L、S/L自由飲用0.2%醋痠鉛溶液,S/C、S/L給予束縳壓力.子代于30 d齡進行Morris水迷宮測試,併檢測海馬組織鉛含量及水迷宮實驗前、後血清腎上腺酮.結果 S/L雄、雌性仔鼠在原平檯所在象限停留時間分彆為(16.08 ±3.41)s、(15.72 ±3.33)s,顯著短于NS/L(25.42±4.76)s、(24.55±4.43)s和S/C(20.96±3.45)s、(20.65±2.98)s,妊娠壓力與鉛對仔鼠在原平檯所在象限停留時間的影響存在交互作用(F=5.478,P<0.05);妊娠壓力與鉛對仔鼠應激後血清腎上腺酮水平的影響存在交互作用.NS/L、S/L仔鼠海馬鉛含量分彆為(0.4378 ±0.1041)μg/g、(0.4679 ±0.1243)μg/g,差異無統計學意義(F=0.298,P0.05).結論 (1)妊娠壓力與鉛對大鼠子代學習記憶的損害可能具有疊加作用.(2)妊娠壓力與鉛對仔鼠下丘腦.垂體-腎上腺軸的影響可能具有疊加作用.該作用可能是二者對子代學習記憶疊加損害的原因之一.(3)聯閤暴露未顯著增加鉛在子代海馬中的蓄積.
목적 탐토임신압력여연연합폭로대대서자대조기공간학습기억능력적영향.방법 운용수자표법수궤장32지Sprague-Dawley잉서분위공백대조조(NS/C),연폭로조(NS/L),압력폭로조(S/C),연합폭로조(S/L),매조8지.NS/L、S/L자유음용0.2%작산연용액,S/C、S/L급여속박압력.자대우30 d령진행Morris수미궁측시,병검측해마조직연함량급수미궁실험전、후혈청신상선동.결과 S/L웅、자성자서재원평태소재상한정류시간분별위(16.08 ±3.41)s、(15.72 ±3.33)s,현저단우NS/L(25.42±4.76)s、(24.55±4.43)s화S/C(20.96±3.45)s、(20.65±2.98)s,임신압력여연대자서재원평태소재상한정류시간적영향존재교호작용(F=5.478,P<0.05);임신압력여연대자서응격후혈청신상선동수평적영향존재교호작용.NS/L、S/L자서해마연함량분별위(0.4378 ±0.1041)μg/g、(0.4679 ±0.1243)μg/g,차이무통계학의의(F=0.298,P0.05).결론 (1)임신압력여연대대서자대학습기억적손해가능구유첩가작용.(2)임신압력여연대자서하구뇌.수체-신상선축적영향가능구유첩가작용.해작용가능시이자대자대학습기억첩가손해적원인지일.(3)연합폭로미현저증가연재자대해마중적축적.
Objective To explore the effects of prenatal exposure to stress and lead on spatial learning and memory development in rats. Methods All 32 Sprague-Dawley (SD) pregnant rats were divided randomly into 4 groups, 8 per group in line with the Random Number Table. The four groups were: no maternal stress, no Pb exposure (NS/C) ; non-maternal stress, Pb exposure (NS/L), maternal stress, no Pb exposure (S/C), and maternal stress plus Pb exposure (S/L). The spatial learning and memory ability, the serum corticosterane level both pre and post-testing of 30-day old offsprings, and lead concentration in hippocampns were tested by means of Morris Water Maze, radioimmunoassay and Inductively Coupled Plaswa Mass Spectrometry(ICP-MS). Results The residence time of male and female in NS/L was ( 16. 08±3.41 ) s, ( 15.72 ± 3.33 ) s, which were significantly shorter than NS/L (25.42 ± 4. 76) s, (24. 55± 4. 43 ) s and S/C ( 20. 96±3.45 ) s, ( 20. 65 ±2. 98 ) s, and significant difference was observed in the joint exposure effect( F = 5. 478, P < 0. 05 ). The effect of the joint exposure was significant on pest-testing serum eorticosterone. The hippocampus lead concentrations of NS/L and S/L were (0. 4378±0. 1041 ) μg/g and ( 0. 4679±0. 1243 ) μg/g without significant differences ( F = 0. 298, P 0. 05). Conclusion Prenatal joint exposure to restraint stress and lead might increase the effects of single exposure on the spatial learning and memory ability and serum corticosterone level of offsprings, and the joint influence on cortieosterone level might be one of the reasons of further impairment of learning and memory.
