中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2010年
3期
411-413
,共3页
小鼠%自发活动%周边活动时间占比%戊巴比妥钠%地西泮%镇静
小鼠%自髮活動%週邊活動時間佔比%戊巴比妥鈉%地西泮%鎮靜
소서%자발활동%주변활동시간점비%무파비타납%지서반%진정
mice%locomotor activity%accumulate peripheral active time percent%pentobarbital sodium%diazepam%sedative effect
目的 建立一种基于累积周边活动时间/周边时间的比值(accumulate peripheral active time percent,APATP)的行为学评价模型.方法 利用视频跟踪系统检测戊巴比妥钠和地西泮诱导小鼠自发活动并提取相应数据,并计算得到APATP.采用DAS软件对数据进行处理,得到小鼠活动趋稳半衰期(T_(1/2α))、对数曲线下面积(lgAUC0-60)和对数尾点回归值(lgPmin)进行统计学分析.结果 各组小鼠的APATP随时间的延长而逐步下降,均符合方程P=Ae~(-αt)+Be~(-βt).与正常组比较,PB 5 mg·kg~(-1)组T_(1/2α)、lgAUC0-60和lgPmin略有下降,而PB 10 mg·kg~(-1)组(均为 P <0.01)和PB 15 mg·kg~(-1) 组(P <0.01, P <0.01和 P >0.05)均能不同程度的抑制3参数;与PB 10 mg·kg~(-1) 组比较,PB 15 mg·kg~(-1)组3参数有所回升(P>0.05,P<0.05和P <0.05).本模型参数lgAUC0-60 和lgPmin与总路程具有较好的一致性(r~2=1.0000和r~2=0.9995,均为P<0.01).应用地西泮进一步证实了模型的可行性,具有类似的特点;与正常组比较,地西泮2、4 mg·kg~(-1)组3参数均下降(均为P<0.01),认为两组的镇静效应与正常等同;与PB 10 mg·kg~(-1)比较,地西泮2、4 mg·kg~(-1) 组的3参数差异均无显著性,认为两组地西泮镇静作用与之等同.结论 ICR小鼠累积周边活动时间占比模型可用于评价小鼠自发活动及药物干预效果.
目的 建立一種基于纍積週邊活動時間/週邊時間的比值(accumulate peripheral active time percent,APATP)的行為學評價模型.方法 利用視頻跟蹤繫統檢測戊巴比妥鈉和地西泮誘導小鼠自髮活動併提取相應數據,併計算得到APATP.採用DAS軟件對數據進行處理,得到小鼠活動趨穩半衰期(T_(1/2α))、對數麯線下麵積(lgAUC0-60)和對數尾點迴歸值(lgPmin)進行統計學分析.結果 各組小鼠的APATP隨時間的延長而逐步下降,均符閤方程P=Ae~(-αt)+Be~(-βt).與正常組比較,PB 5 mg·kg~(-1)組T_(1/2α)、lgAUC0-60和lgPmin略有下降,而PB 10 mg·kg~(-1)組(均為 P <0.01)和PB 15 mg·kg~(-1) 組(P <0.01, P <0.01和 P >0.05)均能不同程度的抑製3參數;與PB 10 mg·kg~(-1) 組比較,PB 15 mg·kg~(-1)組3參數有所迴升(P>0.05,P<0.05和P <0.05).本模型參數lgAUC0-60 和lgPmin與總路程具有較好的一緻性(r~2=1.0000和r~2=0.9995,均為P<0.01).應用地西泮進一步證實瞭模型的可行性,具有類似的特點;與正常組比較,地西泮2、4 mg·kg~(-1)組3參數均下降(均為P<0.01),認為兩組的鎮靜效應與正常等同;與PB 10 mg·kg~(-1)比較,地西泮2、4 mg·kg~(-1) 組的3參數差異均無顯著性,認為兩組地西泮鎮靜作用與之等同.結論 ICR小鼠纍積週邊活動時間佔比模型可用于評價小鼠自髮活動及藥物榦預效果.
목적 건립일충기우루적주변활동시간/주변시간적비치(accumulate peripheral active time percent,APATP)적행위학평개모형.방법 이용시빈근종계통검측무파비타납화지서반유도소서자발활동병제취상응수거,병계산득도APATP.채용DAS연건대수거진행처리,득도소서활동추은반쇠기(T_(1/2α))、대수곡선하면적(lgAUC0-60)화대수미점회귀치(lgPmin)진행통계학분석.결과 각조소서적APATP수시간적연장이축보하강,균부합방정P=Ae~(-αt)+Be~(-βt).여정상조비교,PB 5 mg·kg~(-1)조T_(1/2α)、lgAUC0-60화lgPmin략유하강,이PB 10 mg·kg~(-1)조(균위 P <0.01)화PB 15 mg·kg~(-1) 조(P <0.01, P <0.01화 P >0.05)균능불동정도적억제3삼수;여PB 10 mg·kg~(-1) 조비교,PB 15 mg·kg~(-1)조3삼수유소회승(P>0.05,P<0.05화P <0.05).본모형삼수lgAUC0-60 화lgPmin여총로정구유교호적일치성(r~2=1.0000화r~2=0.9995,균위P<0.01).응용지서반진일보증실료모형적가행성,구유유사적특점;여정상조비교,지서반2、4 mg·kg~(-1)조3삼수균하강(균위P<0.01),인위량조적진정효응여정상등동;여PB 10 mg·kg~(-1)비교,지서반2、4 mg·kg~(-1) 조적3삼수차이균무현저성,인위량조지서반진정작용여지등동.결론 ICR소서루적주변활동시간점비모형가용우평개소서자발활동급약물간예효과.
Aim To establish a novel locomotor model based on accumulate peripheral active time percent(APATP)in ICR mice induced by pentobarbital sodium(PB)and diazepam.Methods Total distance, accumulate peripheral active time and accumulate peripheral time were aquired from video tracking system with computer, and APATP in all groups was fit and got correspondent parameters with Drugs Analysis System 2.0. Logarithm of area under the curve(lgAUC_(0-60)), minimum APATP(lgP_(min)), and half time of steady-state locomotor activity(T_(1/2α)) were ready for evaluation.Results APATP decreased with time increasing in all groups gradually, and PB 10 mg·kg~(-1) and PB 15 mg·kg~(-1) had similar tendency.PB 10 mg·kg~(-1) and PB 15 mg·kg~(-1) all decreased APATP significantly throughout the time course.APATP in all mice was fit to kinetics equation.Compared with norm group, PB 5 mg·kg~(-1) decreased three parameters slightly(all P >0.05), and PB 10 mg·kg~(-1) and PB 15 mg·kg~(-1) had similar tendency.PB 10 mg·kg~(-1) (all P <0.05) and PB 15 mg·kg~(-1) (P <0.01, P <0.01 and P >0.05)decreased parameters.Compared with PB 10 mg·kg~(-1) , PB 15 mg·kg~(-1) increased reversely(P >0.05, P <0.05 and P <0.05).lgAUC_(0-60) and lgPmin were both linar with total distance(r 2=1.0000 and r 2=0.9995, both P <0.01), T_(1/2α) also showed similar tendcency as well as total distance.Refering PB 10 mg·kg~(-1) as positive drugs and norm as negative control, diazepam 2 mg·kg~(-1) and 4 mg·kg~(-1) depressed all parameters significantly compared with norm group(all P <0.01) and were similar as PB 10 mg·kg~(-1) , which indicated that sedative effect of diazepam was the same as PB 10 mg·kg~(-1) .Conclusion Locomotor activity model based on APATP may be used to evaluate drug effects on lomocotor activity induced by sedative hypnotics.
