CAJ | 학술논문

[目的]研究MSTN基因在藏系绵羊不同年龄、不同组织的表达差异.[方法]根据Gen Bank已发表序列(NM_001009428.1)设计1对引物,以藏系绵羊组织总RNA为模板,采用实时荧光定量PCR技术(QRTPCR)分析了MSTN基因在不同年龄阶段的真胃、瘤胃、腿肌和心肌中的基因表达量.[结果]经过内参基因校正后,综合4个组织分析,MSTN基因在6月龄藏系绵羊的相对表达量最高,是12月龄藏系绵羊的2.52倍,是羔羊的2.24倍,是9月龄的1.30倍(P<0.05).且MSTN基因在腿肌中的表达量最高,其他组织几乎未表达,在腿肌中的表达量是其瘤胃的3984.78倍,是心肌的602.69倍(P<0.01).[结论]为MSTN蛋白抗体的正确利用奠定了基础.
[목적]연구MSTN기인재장계면양불동년령、불동조직적표체차이.[방법]근거Gen Bank이발표서렬(NM_001009428.1)설계1대인물,이장계면양조직총RNA위모판,채용실시형광정량PCR기술(QRTPCR)분석료MSTN기인재불동년령계단적진위、류위、퇴기화심기중적기인표체량.[결과]경과내삼기인교정후,종합4개조직분석,MSTN기인재6월령장계면양적상대표체량최고,시12월령장계면양적2.52배,시고양적2.24배,시9월령적1.30배(P<0.05).차MSTN기인재퇴기중적표체량최고,기타조직궤호미표체,재퇴기중적표체량시기류위적3984.78배,시심기적602.69배(P<0.01).[결론]위MSTN단백항체적정학이용전정료기출.
[Objective]The aim was to investigate the difference in MSTN gene expression in different tissues of Tibetan sheep at different ages.[Methed]According to the sequence(NM_001009428.1)published in GenBank, a pair of specific primers was designed to amplify part of cDNA sequence of MSTN by using QRT-PCR technique.The relative expression level of MSTN gene in rennet stomach, rumen, leg muscle and cardiac muscle of Tibetan sheep at different ages were analyzed.[Result]After normalization with β-actin gene, the relative expression level of MSTN gene in the 6-month-old Tibetan sheep was the highest and it was 2.52 times than that in 12-month-old Tibetan sheep(P<0.05), the relative expression level of MSTN gene in leg muscle was the highest among all tissues and it was 3984.78 times than that in rumen(P<0.01).[Conclusion]The results established theoretical foundation for the correct use of MSTN antibody.