CAJ | 학술논문

以9个三叶草品种的上胚轴、下胚轴、叶片和子叶为外植体，研究了影响三叶草愈伤组织诱导和再分化的主要因素。结果表明，不同品种不同外植体愈伤诱导率差异较大，下胚轴为较适宜的外植体，其平均愈伤组织诱导率和愈伤分化率为97％和10．3％，改良SH＋2，4-D8．0mg／L＋6-BA0．2mg／L＋KT0．2mg／L为最佳胚性愈伤组织诱导培养基，所形成的愈伤组织的愈伤分化率也最高。
이9개삼협초품충적상배축、하배축、협편화자협위외식체，연구료영향삼협초유상조직유도화재분화적주요인소。결과표명，불동품충불동외식체유상유도솔차이교대，하배축위교괄의적외식체，기평균유상조직유도솔화유상분화솔위97％화10．3％，개량SH＋2，4-D8．0mg／L＋6-BA0．2mg／L＋KT0．2mg／L위최가배성유상조직유도배양기，소형성적유상조직적유상분화솔야최고。
The Hypocotyls,The Upside,Downside, Leaf and Cotyledon of Nine Varieties of Clover（Trifolum）were Used to Study the Effect of Hormone Concentrations on Callus Induction and Redifferentiation. Different Varieties and Explants Each had Different Induction Requirements. The Downside of Hypocotyl Callus Induction Average Rate of Clover was 97%,While That of Differential Ratio was 10. 3%. The Best Medium for Embryo Genie Callus Induction and Callus Differentiation was Modified SH-2,4-D 8.0 mg/L＋6-BA0.2 mg/L＋KT0.2 mg/L.