CAJ | 학술논문

目的观察过氧化物酶增殖物激活受体γ(PPARγ)在大鼠肾脏衰老过程中的表达分布,探讨其可能作用机制.方法分别以3月龄、12月龄和24月龄SD大鼠为模型,采用Western印迹、免疫组化、原位杂交的方法检测PPARγ蛋白、核酸在不同年龄大鼠肾组织中的表达.结果 PPARγ蛋白在肾组织表达,3月龄大鼠为0.94±0.05,明显高于24月龄大鼠0.78±0.02(t=7.08,P＜0.01),亦高于12月龄大鼠0.87±0.04,但差异无统计学意义(t=2.49,P＞0.05).免疫组化结果显示,PPARγ蛋白在各年龄组大鼠肾小管、集合管上皮细胞中均有分布,主要分布于细胞核内,在老年大鼠肾小球系膜细胞及壁层上皮细胞内也有阳性染色.原位杂交结果显示,PPARγ mRNA的表达分布与免疫组化结果一致.半定量分析显示,在大鼠肾组织的衰老过程中,PPARγ基因表达呈下降趋势.结论 PPARγ作为核转录因子参与了大鼠肾脏衰老过程调控.
목적 관찰과양화물매증식물격활수체γ(PPARγ)재대서신장쇠로과정중적표체분포,탐토기가능작용궤제.방법 분별이3월령、12월령화24월령SD대서위모형,채용Western인적、면역조화、원위잡교적방법검측PPARγ단백、핵산재불동년령대서신조직중적표체.결과 PPARγ단백재신조직표체,3월령대서위0.94±0.05,명현고우24월령대서0.78±0.02(t=7.08,P＜0.01),역고우12월령대서0.87±0.04,단차이무통계학의의(t=2.49,P＞0.05).면역조화결과현시,PPARγ단백재각년령조대서신소관、집합관상피세포중균유분포,주요분포우세포핵내,재노년대서신소구계막세포급벽층상피세포내야유양성염색.원위잡교결과현시,PPARγ mRNA적표체분포여면역조화결과일치.반정량분석현시,재대서신조직적쇠로과정중,PPARγ기인표체정하강추세.결론 PPARγ작위핵전록인자삼여료대서신장쇠로과정조공.
Objective To observe the month age distribution of peroxisome proliferators activated receptor gamma (PPARγ) expression in rat kedney. Methods Wistar rats aged 3 months,12 months and 24 months were made as models who represented young, middle-aged and old group respectively. Western blotting, immunohistochemical (IHC) and in-situ hybridization (ISH) were used to detect the expression and location of protein and mRNA of PPARγ in rat kidney. Results Western blotting results showed that the expression of PPARγ protein was higher in 3 months group than in 24 months group (0.94±0.05 vs. 0.78±0.02, P＜0.01) and 12 months group (0.87±0.04, P＞0.05), and it reduced in 24 months group than in 12 months group (P＞0.05). By IHC,the PPARγ protein was localized predominantly in the nuclear of tubular epithelia and collecting duct cells in each group. In old age group, PPARγ protein was also detected little in the mesangial and Bowman's capsule epithelial cells. Meanwhile, the distribution of PPARγ mRNA with ISH was consistent with above findings. Additional, semi-quantitative analysis of ISH results verified that the level of PPARγ mRNA decreased with ageing. Conclusions As a nuclear transcription factor,PPARγ participates in the regulation of rat kidney aging.