CAJ | 학술논문

目的评价N-myc下游调节基因2(NDRG2)在七氟醚预处理减轻大鼠局灶性脑缺血再灌注损伤中的作用.方法健康雄性成年SD大鼠48只,体重280～320 g,采用随机数字表,将大鼠随机分为3组(n=16):假手术组(S组)、脑缺血再灌注组(I/R组)和七氟醚预处理组(Sev组).采用大脑中动脉阻断法制备大鼠局灶性脑缺血再灌注模型.大鼠吸入2％七氟醚lh,每天1次,连续5d行七氟醚预处理.Sev组于七氟醚预处理结束后24h制备局灶性脑缺血再灌注模型.再灌注24h时行神经功能评分,随后处死大鼠,取脑组织,测定脑梗死体积百分比,采用Western Blot法测定缺血半暗带区NDRG2和活化的Caspase-3的表达,采用免疫组织荧光测定缺血半暗带区NDRG2的表达及定位.结果与S组比较,I/R组和Sev组脑梗死体积百分比升高,神经功能评分降低,脑组织缺血区半暗带NDRG2和活化的Caspase-3表达上调(P＜0.05)；与I/R组比较,Sev组脑梗死体积百分比降低,神经功能评分升高,脑组织缺血区半暗带NDRG2和活化的Caspase-3表达下调(P＜0.05).Sev组核内NDRG2阳性染色较I/R组减浅.结论七氟醚预处理可能通过抑制脑组织NDRG2的表达、活性和细胞凋亡,从而减轻大鼠局灶性脑缺血再灌注损伤.
목적 평개N-myc하유조절기인2(NDRG2)재칠불미예처리감경대서국조성뇌결혈재관주손상중적작용.방법 건강웅성성년SD대서48지,체중280～320 g,채용수궤수자표,장대서수궤분위3조(n=16):가수술조(S조)、뇌결혈재관주조(I/R조)화칠불미예처리조(Sev조).채용대뇌중동맥조단법제비대서국조성뇌결혈재관주모형.대서흡입2％칠불미lh,매천1차,련속5d행칠불미예처리.Sev조우칠불미예처리결속후24h제비국조성뇌결혈재관주모형.재관주24h시행신경공능평분,수후처사대서,취뇌조직,측정뇌경사체적백분비,채용Western Blot법측정결혈반암대구NDRG2화활화적Caspase-3적표체,채용면역조직형광측정결혈반암대구NDRG2적표체급정위.결과 여S조비교,I/R조화Sev조뇌경사체적백분비승고,신경공능평분강저,뇌조직결혈구반암대NDRG2화활화적Caspase-3표체상조(P＜0.05)；여I/R조비교,Sev조뇌경사체적백분비강저,신경공능평분승고,뇌조직결혈구반암대NDRG2화활화적Caspase-3표체하조(P＜0.05).Sev조핵내NDRG2양성염색교I/R조감천.결론 칠불미예처리가능통과억제뇌조직NDRG2적표체、활성화세포조망,종이감경대서국조성뇌결혈재관주손상.
Objective To investigate the role of N-myc downstream regulated genes 2 (NDRG2) in attenuation of focal cerebral ischemic-reperfusin injury by sevoflurane preconditioning in rats.Methods Forty-eight healthy male SD rats weighing 280-320 g were randomly divided into 3 groups ( n =16 each):sham operation group (group S),ischemia-reperfusion injury group (group I/R) and sevoflurane preconditioning group (group Sev).Focal cerebral ischemia-reperfusion injury was induced by right middle cerebral artery occlusion (MCAO)for 120 min followed by 24 h reperfusion.In group Sev,2.0％ sevoflurane was inhaled 1 h once a day for 5 consecutive days at 24 h before MCAO.The neurologic function was evaluated at 24 h of reperfusion and than the rats were sacrificed,and the brain was removed for determination of infarct volume percentage,NDRG2 and activated Caspase-3 expression in ischemic penumbra by Western Blot and NDRG2 expression and location by immunohistochemistry.Results The infarct volume percentage,NDRG2 and activated Caspase-3 expression were higher,and neurologic function score was lower in groups I/R and Sev then in group S( P ＜ 0.05).The infarct volume percentage,NDRG2 and activated Caspase-3 expression were lower,and neurologic function score was higher in group Sev then in group I/R ( P ＜ 0.05).The intranuclear NDRG2 positive staining was decreased in group Sev than in group I/R.Conclusion Sevoflurane preconditioning can reduce focal cerebral ischemia-reperfusion injury by inhibiting the expression and activity of NDRG2 and apoptosis in rats.