中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2011年
12期
928-930
,共3页
王心%尚丽新%张茜%徐晓东%霍晓溪
王心%尚麗新%張茜%徐曉東%霍曉溪
왕심%상려신%장천%서효동%곽효계
二乙基己基邻苯二甲酸%锌%妊娠,动物
二乙基己基鄰苯二甲痠%鋅%妊娠,動物
이을기기기린분이갑산%자%임신,동물
Diethylhexyl phthlate%Zinc%Pregnancy,animal
目的 探讨邻苯二甲酸(2-乙基己基)酯(DEHP)对大鼠妊娠结局的影响及锌对其影响的保护作用.方法 将50只雌鼠随机分为DEHP组(50mg·kg-1 ·d-1)、DEHP+锌组(含1.2 mg锌的葡萄糖酸锌+ 50 mg·kg-·d-1 DEHP)、空白组(灌注1ml生理盐水)、玉米油组(灌注1ml玉米油)、锌组(含1.2 mg锌的葡萄糖酸锌1ml)5组,每组10只,孕前7d开始每日给予相应药物灌胃,直至孕19 d处死孕鼠,取出胎鼠.测量孕鼠体质量、各脏器质量、活胎鼠数、活胎鼠体质量及胎盘质量.结果 孕鼠体质量及肾、脾、脑、心脏等脏器质量指数各组间两两比较,差异均无统计学意义(P＞0.05).孕鼠肝、子宫及卵巢质量指数,空白组分别为(4.4±0.7)％、(1.26±0.09)％、(0.083±0.009)％,玉米油组分别为(4.5±0.6)％、(1.29±0.10)％、(0.084±0.008)％,锌组分别为(4.4±0.4)％、(1.26±0.08)％、(0.084±0.009)％,DEHP组分别为(5.4±1.0)％、(1.11±0.08)％、(0.074±0.012)％,DEHP+锌组分别为(4.4±1.0)％、(1.28±0.10)％、(0.082±0.007)％,DEHP组与其他4组两两比较,差异均有统计学意义(P＜0.05),其他4组间两两比较,差异均无统计学意义(P＞0.05).活胎鼠数、活胎鼠体质量及胎盘质量,空白组分别为(12.8±2.7)只、(6.03±0.16)g、(1.00±0.03)g,玉米油组分别为(13.6±3.1)只、(6.07±0.20)g、(1.00±0.04)g,锌组分别为(13.3±3.1)只、(6.16 ±0.18)g、(1.00±0.05)g,DEHP组分别为(9.2±4.1)只、(4.03±0.09)g、(0.95±0.03)g,DEHP+锌组分别为(12.1±2.9)只、(6.09±0.17)g、(0.99±0.03)g,DEHP组与其他4组两两比较,差异均有统计学意义(P＜0.05),其他4组间两两比较,差异均无统计学意义(P＞0.05).结论 妊娠期DEHP的暴露可对孕鼠及胎鼠造成损伤,预先给予锌干预可减轻DEHP暴露对妊娠的影响,保护妊娠过程.
目的 探討鄰苯二甲痠(2-乙基己基)酯(DEHP)對大鼠妊娠結跼的影響及鋅對其影響的保護作用.方法 將50隻雌鼠隨機分為DEHP組(50mg·kg-1 ·d-1)、DEHP+鋅組(含1.2 mg鋅的葡萄糖痠鋅+ 50 mg·kg-·d-1 DEHP)、空白組(灌註1ml生理鹽水)、玉米油組(灌註1ml玉米油)、鋅組(含1.2 mg鋅的葡萄糖痠鋅1ml)5組,每組10隻,孕前7d開始每日給予相應藥物灌胃,直至孕19 d處死孕鼠,取齣胎鼠.測量孕鼠體質量、各髒器質量、活胎鼠數、活胎鼠體質量及胎盤質量.結果 孕鼠體質量及腎、脾、腦、心髒等髒器質量指數各組間兩兩比較,差異均無統計學意義(P＞0.05).孕鼠肝、子宮及卵巢質量指數,空白組分彆為(4.4±0.7)％、(1.26±0.09)％、(0.083±0.009)％,玉米油組分彆為(4.5±0.6)％、(1.29±0.10)％、(0.084±0.008)％,鋅組分彆為(4.4±0.4)％、(1.26±0.08)％、(0.084±0.009)％,DEHP組分彆為(5.4±1.0)％、(1.11±0.08)％、(0.074±0.012)％,DEHP+鋅組分彆為(4.4±1.0)％、(1.28±0.10)％、(0.082±0.007)％,DEHP組與其他4組兩兩比較,差異均有統計學意義(P＜0.05),其他4組間兩兩比較,差異均無統計學意義(P＞0.05).活胎鼠數、活胎鼠體質量及胎盤質量,空白組分彆為(12.8±2.7)隻、(6.03±0.16)g、(1.00±0.03)g,玉米油組分彆為(13.6±3.1)隻、(6.07±0.20)g、(1.00±0.04)g,鋅組分彆為(13.3±3.1)隻、(6.16 ±0.18)g、(1.00±0.05)g,DEHP組分彆為(9.2±4.1)隻、(4.03±0.09)g、(0.95±0.03)g,DEHP+鋅組分彆為(12.1±2.9)隻、(6.09±0.17)g、(0.99±0.03)g,DEHP組與其他4組兩兩比較,差異均有統計學意義(P＜0.05),其他4組間兩兩比較,差異均無統計學意義(P＞0.05).結論 妊娠期DEHP的暴露可對孕鼠及胎鼠造成損傷,預先給予鋅榦預可減輕DEHP暴露對妊娠的影響,保護妊娠過程.
목적 탐토린분이갑산(2-을기기기)지(DEHP)대대서임신결국적영향급자대기영향적보호작용.방법 장50지자서수궤분위DEHP조(50mg·kg-1 ·d-1)、DEHP+자조(함1.2 mg자적포도당산자+ 50 mg·kg-·d-1 DEHP)、공백조(관주1ml생리염수)、옥미유조(관주1ml옥미유)、자조(함1.2 mg자적포도당산자1ml)5조,매조10지,잉전7d개시매일급여상응약물관위,직지잉19 d처사잉서,취출태서.측량잉서체질량、각장기질량、활태서수、활태서체질량급태반질량.결과 잉서체질량급신、비、뇌、심장등장기질량지수각조간량량비교,차이균무통계학의의(P＞0.05).잉서간、자궁급란소질량지수,공백조분별위(4.4±0.7)％、(1.26±0.09)％、(0.083±0.009)％,옥미유조분별위(4.5±0.6)％、(1.29±0.10)％、(0.084±0.008)％,자조분별위(4.4±0.4)％、(1.26±0.08)％、(0.084±0.009)％,DEHP조분별위(5.4±1.0)％、(1.11±0.08)％、(0.074±0.012)％,DEHP+자조분별위(4.4±1.0)％、(1.28±0.10)％、(0.082±0.007)％,DEHP조여기타4조량량비교,차이균유통계학의의(P＜0.05),기타4조간량량비교,차이균무통계학의의(P＞0.05).활태서수、활태서체질량급태반질량,공백조분별위(12.8±2.7)지、(6.03±0.16)g、(1.00±0.03)g,옥미유조분별위(13.6±3.1)지、(6.07±0.20)g、(1.00±0.04)g,자조분별위(13.3±3.1)지、(6.16 ±0.18)g、(1.00±0.05)g,DEHP조분별위(9.2±4.1)지、(4.03±0.09)g、(0.95±0.03)g,DEHP+자조분별위(12.1±2.9)지、(6.09±0.17)g、(0.99±0.03)g,DEHP조여기타4조량량비교,차이균유통계학의의(P＜0.05),기타4조간량량비교,차이균무통계학의의(P＞0.05).결론 임신기DEHP적폭로가대잉서급태서조성손상,예선급여자간예가감경DEHP폭로대임신적영향,보호임신과정.
Objective To investigate the effect of di-( 2-ethylhexyl ) phthalate (DEHP) on pregnant rat and the protection of zinc.Methods Fifty rats were randomized equally into 5 groups consisting of blank (given 1 ml 0.9％ sodium chloride),corn oil (given 1 ml corn oil),zinc (given 1 ml zinc gluconate including 1.2 mg zinc),DEHP (given 50 mg · kg-1 · d-1 DEHP)and DEHP + zinc (given 50 mg· kg-1 · d-1 DEHP + zinc gluconate ).At the beginning of the experiment (about 7 d before pregnancy),the female rats were administered with corresponding drugs everyday.The pregnant rats were killed and the fetal rats were removed on 19th day.The following results in each group were recorded:the body weight and the organic weight of the female rats,the number and the weight of fetus rats and the placental weight.Results The weight and the coefficient of female rats' kidney/body,spleen/body,brain/ body,and heart/body in DEHP group compared with other groups were not statistical significance (all P ＞0.05).The coefficient of female rats' liver/body,uterus/body,and ovary/body of blank group were (4.4 ± 0.7 ) ％,( 1.26 ± 0.09 ) ％,( 0.083 ± 0.009 ) ％ respectively,corn oil group were (4.5 ± 0.6 ) ％,( 1.29 ±0.10)％,(0.084 ±0.008)％,zinc group were (4.4 ±0.4)％,( 1.26 ±0.08)％,(0.084 ± 0.009 ) ％,DEHP group were ( 5.4 ± 1.0 ) ％,( 1.11 ± 0.08 ) ％,( 0.074 ± 0.012 ) ％,and DEHP + zinc group were (4.4 ± 1.0)％,( 1.28 ± 0.10)％,(0.082 ± 0.007)％ ; in DEHP group the coefficient of female rats' liver/body,uterus/body,and ovary/body compared with other groups was statistical significance ( P ＜ 0.05 ).The fetal quantity,fetal weight and placental weight of female rats of blank group were 12.8 ± 2.7,(6.03 ±0.16) g,( 1.00 ±0.03) g respectively,corn oil group were 13.6 ±3.1,(6.07 ±0.20) g,(1.00±0.04) g,zinc group were 13.3 ±3.1,(6.16 ±0.18) g,( 1.00 ±0.05) g,DEHP group were 9.2±4.1,(4.03 ±0.09) g,(0.95 ±0.03) g,and zinc +DEHP group were 12.1 ±2.9,(6.09 ± 0.17 ) g,(0.99 ±0.03 ) g.In DEHP group the fetal quantity,fetal weight and placental weight of female rats compared with other groups were statistical significance ( P ＜ 0.05 ).Conclusion DEHP can damage female rats and fetal rats in gestation period.Zinc supplied before pregnancy can relieve the influence by DEHP.
