中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
2期
177-179,封3
,共4页
钱毅%李建平%顾元龙%何友钊%胡浩
錢毅%李建平%顧元龍%何友釗%鬍浩
전의%리건평%고원룡%하우쇠%호호
脾切除术%诱导型一氧化氮合酶%肝脏肿瘤%门静脉高压症
脾切除術%誘導型一氧化氮閤酶%肝髒腫瘤%門靜脈高壓癥
비절제술%유도형일양화담합매%간장종류%문정맥고압증
Splenectomy%Inducible nitric oxide synthase%Hepatic tumor%Portal hypertension
目的 观察脾切除对肝前性门静脉高压大鼠肝脏种植型肿瘤组织的诱导型一氧化氮合酶(iNOS)mRNA表达和肿瘤微血管密度(MVD)的影响.方法 采用门静脉部分结扎的方法 建立20只肝前性门静脉高压大鼠的模型,3周后将其随机分为2组:施行脾切除手术组(A组)、不施行脾切除手术组(B组),脾切除1周后在A、B组大鼠的肝脏上种植Walker256瘤株,2周后肿瘤组织取材.逆转录-聚合酶链反应(RT-PCR)方法 测定各组肿瘤组织标本的诱导型iNOS mRNA的表达量;免疫组织化学方法 染色进行肿瘤微血管计数;测定A、B组大鼠肝脏种植肿瘤的最长、最短径,计算肿瘤体积.结果 A、B两组iNOS mRNA表达量分别为2.32±0.24、2.98±0.28(P<0.05),A、B两组的肿瘤MVD分别为(18.76±1.21)/HP、(19.98±1.28)/HP(P<0.05),iNOS mRNA的表达量及肿瘤MVD的变化呈正相关(r=0.4751,P<0.05).结论 施行脾切除术可以降低肝前性门静脉高压大鼠肝脏种植型肿瘤iNOS mRNA的表达,导致一氧化氮(NO)的合成、释放减少,削弱了肿瘤血管生成的促进因素,使肿瘤组织MVD降低.该手术有助于降低肝脏肿瘤的侵袭能力.
目的 觀察脾切除對肝前性門靜脈高壓大鼠肝髒種植型腫瘤組織的誘導型一氧化氮閤酶(iNOS)mRNA錶達和腫瘤微血管密度(MVD)的影響.方法 採用門靜脈部分結扎的方法 建立20隻肝前性門靜脈高壓大鼠的模型,3週後將其隨機分為2組:施行脾切除手術組(A組)、不施行脾切除手術組(B組),脾切除1週後在A、B組大鼠的肝髒上種植Walker256瘤株,2週後腫瘤組織取材.逆轉錄-聚閤酶鏈反應(RT-PCR)方法 測定各組腫瘤組織標本的誘導型iNOS mRNA的錶達量;免疫組織化學方法 染色進行腫瘤微血管計數;測定A、B組大鼠肝髒種植腫瘤的最長、最短徑,計算腫瘤體積.結果 A、B兩組iNOS mRNA錶達量分彆為2.32±0.24、2.98±0.28(P<0.05),A、B兩組的腫瘤MVD分彆為(18.76±1.21)/HP、(19.98±1.28)/HP(P<0.05),iNOS mRNA的錶達量及腫瘤MVD的變化呈正相關(r=0.4751,P<0.05).結論 施行脾切除術可以降低肝前性門靜脈高壓大鼠肝髒種植型腫瘤iNOS mRNA的錶達,導緻一氧化氮(NO)的閤成、釋放減少,削弱瞭腫瘤血管生成的促進因素,使腫瘤組織MVD降低.該手術有助于降低肝髒腫瘤的侵襲能力.
목적 관찰비절제대간전성문정맥고압대서간장충식형종류조직적유도형일양화담합매(iNOS)mRNA표체화종류미혈관밀도(MVD)적영향.방법 채용문정맥부분결찰적방법 건립20지간전성문정맥고압대서적모형,3주후장기수궤분위2조:시행비절제수술조(A조)、불시행비절제수술조(B조),비절제1주후재A、B조대서적간장상충식Walker256류주,2주후종류조직취재.역전록-취합매련반응(RT-PCR)방법 측정각조종류조직표본적유도형iNOS mRNA적표체량;면역조직화학방법 염색진행종류미혈관계수;측정A、B조대서간장충식종류적최장、최단경,계산종류체적.결과 A、B량조iNOS mRNA표체량분별위2.32±0.24、2.98±0.28(P<0.05),A、B량조적종류MVD분별위(18.76±1.21)/HP、(19.98±1.28)/HP(P<0.05),iNOS mRNA적표체량급종류MVD적변화정정상관(r=0.4751,P<0.05).결론 시행비절제술가이강저간전성문정맥고압대서간장충식형종류iNOS mRNA적표체,도치일양화담(NO)적합성、석방감소,삭약료종류혈관생성적촉진인소,사종류조직MVD강저.해수술유조우강저간장종류적침습능력.
Objective To evaluate the role of splenectomy in inducible nitric oxide synthase (iNOS) expression and microvessal density (MVD) in implanted hepatic tumor model of prehepatic portal hypertension rats. Methods The prehepatic portal hypertension rats were divided randomly into two groups: The rats in group A were subjected to splenectomy one week before implantation of tumor tissues; the rats in group B were not subjected to spleuectomy but sham operation one week before implantation of tumor tissues. Walker-256 tumor tissues were implanted in livers of group A and group B. Two weeks after tumor tissue implantation, the samples of tumor tissue were harvested. The expression of iNOS mRNA in two groups was detected by reverse transcription pelymerase chain reaction. MVD was counted by irnmunohistochem-istric staining with CD34 antibody. The volumes of tumor were measure by vernier caliper. Results In group B the iNOS expression was elevated significantly in eomparision with group A (P<0.05). MVD in groups B and A was (19.98±1.28)/HP, and (18.76±1.21)/HP respectively (P<0.05). A positive correlation between iNOS expression and MVD was observed (P<0.05). Although the average volume of tumors in group B (867.05±90.54) mm~3 was seemed lardger than that in group A (859.35±91.18) mm~3, there was no significant different in statistics between the two groups (P>0.05). Conclnsion After splenectomy, the expression of iNOS in the implanted hepatic tumor in prehepatic portal hypertension rats was decreased in comparison with that not subject to splenectomy. The lower expression of iNOS could lead to the lower products of NO, which reduced the stimulators in tumor angiogenesis, resulting in the decrease of MVD in tumor tissue. Spleuectomy on prehepatic portal hypertension rats can decrease the invasive abilities of malignant hepatic tumor.
