中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2008年
4期
252-254
,共3页
徐新女%王金环%王淑杰%郭承承%刘宏胜%卢轶%张益伟%白雪
徐新女%王金環%王淑傑%郭承承%劉宏勝%盧軼%張益偉%白雪
서신녀%왕금배%왕숙걸%곽승승%류굉성%로질%장익위%백설
神经胶质瘤%富亮氨酸胶质瘤失活基因1(LGI1)%逆转录聚合酶链反应%Ki-67
神經膠質瘤%富亮氨痠膠質瘤失活基因1(LGI1)%逆轉錄聚閤酶鏈反應%Ki-67
신경효질류%부량안산효질류실활기인1(LGI1)%역전록취합매련반응%Ki-67
Glioma%Leucine-rich gene glioma inactivated 1%RT-PCR%Ki-67
目的 探讨胶质瘤组织富亮氨酸胶质瘤失活基因1(LGI1)mRNA的表达及与细胞增殖活性之间的关系.方法 采用逆转录聚合酶链反应(RT-PCR)法对30例脑胶质瘤组织、2例脑膜瘤、2例瘤旁及2例颅脑损伤内减压脑组织标本进行半定量检测,同时采用S-P免疫组化法检测Ki-67标记指数.结果 脑胶质瘤组的LGI1 mRNA表达水平低于正常脑组织和脑膜瘤(P＜0.05).脑胶质瘤中,WHO Ⅳ级的胶质瘤LGll mRNA表达低于WHOⅡ级和Ⅲ级胶质瘤(P＜0.05),与Ki-67标记指数呈负相关关系(r=-0.621,P＜0.01).结论 LGI1基因的表达与Ki-67标记指数呈负相关,提示LGI1与胶质瘤的发生关系密切.
目的 探討膠質瘤組織富亮氨痠膠質瘤失活基因1(LGI1)mRNA的錶達及與細胞增殖活性之間的關繫.方法 採用逆轉錄聚閤酶鏈反應(RT-PCR)法對30例腦膠質瘤組織、2例腦膜瘤、2例瘤徬及2例顱腦損傷內減壓腦組織標本進行半定量檢測,同時採用S-P免疫組化法檢測Ki-67標記指數.結果 腦膠質瘤組的LGI1 mRNA錶達水平低于正常腦組織和腦膜瘤(P＜0.05).腦膠質瘤中,WHO Ⅳ級的膠質瘤LGll mRNA錶達低于WHOⅡ級和Ⅲ級膠質瘤(P＜0.05),與Ki-67標記指數呈負相關關繫(r=-0.621,P＜0.01).結論 LGI1基因的錶達與Ki-67標記指數呈負相關,提示LGI1與膠質瘤的髮生關繫密切.
목적 탐토효질류조직부량안산효질류실활기인1(LGI1)mRNA적표체급여세포증식활성지간적관계.방법 채용역전록취합매련반응(RT-PCR)법대30례뇌효질류조직、2례뇌막류、2례류방급2례로뇌손상내감압뇌조직표본진행반정량검측,동시채용S-P면역조화법검측Ki-67표기지수.결과 뇌효질류조적LGI1 mRNA표체수평저우정상뇌조직화뇌막류(P＜0.05).뇌효질류중,WHO Ⅳ급적효질류LGll mRNA표체저우WHOⅡ급화Ⅲ급효질류(P＜0.05),여Ki-67표기지수정부상관관계(r=-0.621,P＜0.01).결론 LGI1기인적표체여Ki-67표기지수정부상관,제시LGI1여효질류적발생관계밀절.
Objective To analyze the expression of the mRNA of LGI1 in human glioma tissues and to explore the relationship between the expression of the mRNA of LGI1 and cell proliferation.Method The tissue specimens of 30 gliomas,2 para-tumors,2 meningiomas and 2 normal brains from cerebral injury patients with Intracranial decompression were collected from the Neurosurgery Department of Tianjin First Central Hospital and the Tianjin Huanhu Hospital.Special primers were designed according to related exons of LGI1.Six exons were amplified by reverse transcriptase polymerase chain reaction(RT-PCR)and the expression level of LGI1 mRNA was detected by semi-quantify. Ki-67 LI was detected by immunohistochemical analysis. Results The expression level of LGI1 mRNA in group of gliomas was significantly lower than that in group of normal tissues or meningomas(P＜0.05).The expression level of LGI1 mRNA in the gliomas of grade Ⅳ was lower than that in the glioma of grade Ⅱ/Ⅲ(P＜0.05).The expresson of LGI1 had a negative correlation with WHO grade and Ki-67 LI(r=-0.621,P＜0.01).Conclusion LGI1 gene is a tumor suppressor gene,the expression of LGI1 gene has a close correlation with the progress of glioma.
