CAJ | 학술논문

通过水培试验,研究比较了不同浓度(10～60 mg/L)的Cr3+、Cr6+胁迫对茶树叶片一些生理生化指标的影响.结果表明:随Cr3+、Cr6+胁迫浓度的增加,茶树受害程度加深,中毒症状明显;叶绿素含量以及叶绿素a/b比值极显著降低,并对净光合速率、胞间CO2浓度、蒸腾速率及气孔导度产生显著负面影响.Cr3+胁迫下,SOD、POD、CAT活性随Cr3+浓度增加呈先升高后降低趋势;而Cr6+胁迫下,SOD、POD活性也出现先升后降趋势,但CAT活性则持续下降.丙二醛含量、细胞膜透性和脯氨酸含量也随Cr3+、Cr6+浓度增加显著增加,表明Cr3+、Cr6+胁迫对茶树细胞质膜系统及主要细胞器的结构与功能都具有较强的破坏作用,Cr6+的毒害作用强于Cr3+.
통과수배시험,연구비교료불동농도(10～60 mg/L)적Cr3+、Cr6+협박대다수협편일사생리생화지표적영향.결과표명:수Cr3+、Cr6+협박농도적증가,다수수해정도가심,중독증상명현;협록소함량이급협록소a/b비치겁현저강저,병대정광합속솔、포간CO2농도、증등속솔급기공도도산생현저부면영향.Cr3+협박하,SOD、POD、CAT활성수Cr3+농도증가정선승고후강저추세;이Cr6+협박하,SOD、POD활성야출현선승후강추세,단CAT활성칙지속하강.병이철함량、세포막투성화포안산함량야수Cr3+、Cr6+농도증가현저증가,표명Cr3+、Cr6+협박대다수세포질막계통급주요세포기적결구여공능도구유교강적파배작용,Cr6+적독해작용강우Cr3+.
The toxic effects of different concentrations of Cr3+ and Cr6+ on the physiological and biochemical characteristics of tea plant were studied in Hoagland solution culture.The results indicated that: with the increasing of Cr3+,Cr6+ concentrations,the symptoms of tea plant became obviously; the contents of chlorophyll and the ratio of chlorophyll a and chlorophyll b reduced obviously,and there were obviously negative effects to net photosynthesis rate,intercellular CO2 concentration,transpiration rate and stoma conductivity.Under the Cr3+ stress,the activities of SOD,POD and CAT increased at first and then decreased,under the Cr6+ stress,the activities of SOD and POD increased at first and then decreased too,but the activity of CAT decreased all the time.At the same time,the contents of MDA and proline (Pro),cytomembrane permeability increased obviously with the increasing of Cr3+,Cr6+ concentrations.Therefore,it highlighted that the Cr3+ and Cr6+ stresses could mangle the cytomembrane system,the structures and functions of the major organelles of tea plant,and the toxic effects of Cr6+ on tea plant was stronger than that of Cr3+.