合肥工业大学学报（自然科学版）
閤肥工業大學學報（自然科學版）
합비공업대학학보（자연과학판）
JOURNAL OF HEFEI UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE)
2009年
11期
1730-1734
,共5页
叶明%叶崇军%胡士明%刘惠东%贾光蕾%王凤美
葉明%葉崇軍%鬍士明%劉惠東%賈光蕾%王鳳美
협명%협숭군%호사명%류혜동%가광뢰%왕봉미
纤维素酶%16SrDNA%复合诱变%产酶条件%益生菌
纖維素酶%16SrDNA%複閤誘變%產酶條件%益生菌
섬유소매%16SrDNA%복합유변%산매조건%익생균
cellulase%16S rDNA%composite mutation%condition of cellulase production%probiotics
从土壤中筛选出一株产纤维素酶的细菌B1,通过形态观察、生理生化特征及16S rDNA同源性序列分析,鉴定为蜡样芽孢杆菌(Bacillus cereus).经微波-硫酸二乙酯复合诱变筛选出正突变株B1-1,其纤维素酶酶活达到0.373 U/mL,经5次传代后,该菌株遗传性状稳定,且胃肠道耐受性良好;B1-1菌株最佳产酶条件为20 g/L麦芽糖,2.5 g/L硫酸铵,pH值为5.0,37 ℃下培养36 h.
從土壤中篩選齣一株產纖維素酶的細菌B1,通過形態觀察、生理生化特徵及16S rDNA同源性序列分析,鑒定為蠟樣芽孢桿菌(Bacillus cereus).經微波-硫痠二乙酯複閤誘變篩選齣正突變株B1-1,其纖維素酶酶活達到0.373 U/mL,經5次傳代後,該菌株遺傳性狀穩定,且胃腸道耐受性良好;B1-1菌株最佳產酶條件為20 g/L麥芽糖,2.5 g/L硫痠銨,pH值為5.0,37 ℃下培養36 h.
종토양중사선출일주산섬유소매적세균B1,통과형태관찰、생리생화특정급16S rDNA동원성서렬분석,감정위사양아포간균(Bacillus cereus).경미파-류산이을지복합유변사선출정돌변주B1-1,기섬유소매매활체도0.373 U/mL,경5차전대후,해균주유전성상은정,차위장도내수성량호;B1-1균주최가산매조건위20 g/L맥아당,2.5 g/L류산안,pH치위5.0,37 ℃하배양36 h.
Strain Bl with cellulase activity was screened from soil, which was identified as Bacillus cereus according to the characteristics of morphology, physiology and biochemistry tests and the comparison of 16S rD-NA sequence. Strain Bl-1 with high cellulase activity was filtrated by microwave and DES mutagenesis, and its cellulase activity reached 0. 373 U/mL. Strain Bl-1 was genetically stable after five subcultures and well survived in the gastrointestinal tract The optimum conditions for cellulase production were 20 g/L maltose, 2. 5 g/L ammonium sulphate, pH 5. 0 and culturing for 36 h at 37℃.
