北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2010年
1期
47-50
,共4页
大蒜素%弓形虫%感染%黏膜免疫%小鼠
大蒜素%弓形蟲%感染%黏膜免疫%小鼠
대산소%궁형충%감염%점막면역%소서
allicin%Toxoplasma gondii%infection%mucous immunity%mice
目的 通过测定弓形虫感染小鼠大蒜素治疗后黏膜部位SIgA含量,研究大蒜素在弓形虫感染免疫中所起作用.方法 将雌性小鼠分成治疗组、感染组和对照组,用5×10~4个/只RH株弓形虫速殖子经口感染小鼠后大蒜素治疗,每天1次,于治疗后第0、2、4、6、8、10、12周断颈处死,冲洗上呼吸道、下呼吸道、肠道、阴道、子宫黏膜,夹心ELISA法测定SIgA抗体含量.结果 在观察的时间段内,治疗组SIgA含量高于对照组和感染组,治疗后4、6、8周差异具有显著性(P＜0.05).结论 大蒜素治疗经口感染弓形虫速殖子小鼠,能提高黏膜(特别是消化道黏膜)SIgA抗体含量,保护机体抵御病原体感染.
目的 通過測定弓形蟲感染小鼠大蒜素治療後黏膜部位SIgA含量,研究大蒜素在弓形蟲感染免疫中所起作用.方法 將雌性小鼠分成治療組、感染組和對照組,用5×10~4箇/隻RH株弓形蟲速殖子經口感染小鼠後大蒜素治療,每天1次,于治療後第0、2、4、6、8、10、12週斷頸處死,遲洗上呼吸道、下呼吸道、腸道、陰道、子宮黏膜,夾心ELISA法測定SIgA抗體含量.結果 在觀察的時間段內,治療組SIgA含量高于對照組和感染組,治療後4、6、8週差異具有顯著性(P＜0.05).結論 大蒜素治療經口感染弓形蟲速殖子小鼠,能提高黏膜(特彆是消化道黏膜)SIgA抗體含量,保護機體牴禦病原體感染.
목적 통과측정궁형충감염소서대산소치료후점막부위SIgA함량,연구대산소재궁형충감염면역중소기작용.방법 장자성소서분성치료조、감염조화대조조,용5×10~4개/지RH주궁형충속식자경구감염소서후대산소치료,매천1차,우치료후제0、2、4、6、8、10、12주단경처사,충세상호흡도、하호흡도、장도、음도、자궁점막,협심ELISA법측정SIgA항체함량.결과 재관찰적시간단내,치료조SIgA함량고우대조조화감염조,치료후4、6、8주차이구유현저성(P＜0.05).결론 대산소치료경구감염궁형충속식자소서,능제고점막(특별시소화도점막)SIgA항체함량,보호궤체저어병원체감염.
Objective To study the effect of allicin in the immunity of Toxoplasma gondii infection through the quantitative determination of mucous secretory IgA (SIgA) in mice infected by Toxoplasma gondii after the treatment with allicin.Methods All female mice were divided into the treatment group, infection group and control group. The mice were infected by tachyzoites of Toxoplasma gondii RH strain (5×10~4/each) through mouth and then treated with allicin once a day. On the 0~(th), 2~(nd), 4~(th), 6~(th), 8~(th), 10~(th) and 12~(th) day respectively after the treatment the mice were killed and irrigated in the upper and lower respiratory tract, intestines, vagina and endometrium. The content of SIgA was detected by using enzyme-linked immunosorbent assay (ELISA).Results In the observation duration SIgA content was higher in the treatment group than that in the control group or infection group. The difference was significant (P<0.05) in 4, 6 and 8 weeks after the treatment.Conclusion Allicin, used to treat the mice infected by tachyzoites of Toxoplasma gondii RH strain through mouth, can increase the content of SIgA in mucosa especially in digestive mucosa to protect the body from the infection of pathogen.
