CAJ | 학술논문

目的对候选抑癌基因DOC-1R((Deleted in oral cancer-1 related,CDK2AP2)相关基因DCT1(DOC-1B terminal 1)进行染色体定位分析并观察其细胞内定位,检测该基因在人类组织中的表达.方法通过辐射杂交细胞系对其进行染色体定位分析,同时构建EGFP-DCT1质粒并转染HeLa细胞,荧光显微镜观察DCT1蛋白在细胞内的定位.此外,应用荧光定量PCR的方法检测该基因在16个正常人类组织中的表达.结果 DCT1基因定位于5q31,其编码产物在HeLa细胞中定位于核膜,该基因在正常人类的16个组织中均有表达,其中在脾中表达水平较高.结论 DCT1可能为细胞周期调控相关基因,在人类组织中普遍表达.
목적 대후선억암기인DOC-1R((Deleted in oral cancer-1 related,CDK2AP2)상관기인DCT1(DOC-1B terminal 1)진행염색체정위분석병관찰기세포내정위,검측해기인재인류조직중적표체.방법 통과복사잡교세포계대기진행염색체정위분석,동시구건EGFP-DCT1질립병전염HeLa세포,형광현미경관찰DCT1단백재세포내적정위.차외,응용형광정량PCR적방법검측해기인재16개정상인류조직중적표체.결과 DCT1기인정위우5q31,기편마산물재HeLa세포중정위우핵막,해기인재정상인류적16개조직중균유표체,기중재비중표체수평교고.결론 DCT1가능위세포주기조공상관기인,재인류조직중보편표체.
Objective To investigate the chromosomal and subcellular localization of DOC-1R terminal 1(DCT1),and detect its expression in human tissues.Methods Chromosome localization of DCT1 was detected by radiation hybrid.pEGFP-DCT1 was constructed,and HeLa cells were transfected with the plasmid.The subcellular localization of DCT1 protein was observed by fluorescence microscope.Real-time PCR was performed for the determination of DCT1 expression level in 16 kinds of human tissues.Results DCT1 was demonstrated to localize in 5q31,and its encoding protein was detected on the nuclear membrane.Additionally,DCT1 was proved to express universally in all the 16 kinds of human tissues and it was expressed at the highest level in spleen.Conclusion DCT1 might be a regulator in cell cycle,and ubiquitously express in human tissues.