国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2011年
8期
689-691
,共3页
牛磺酸%神经元%干细胞因子%缺血再灌注损伤
牛磺痠%神經元%榦細胞因子%缺血再灌註損傷
우광산%신경원%간세포인자%결혈재관주손상
Taurine%Neurons%Neural stem cell factor%Ischmia reperfusion injury
目的 观察牛磺酸对大鼠缺血性脑损伤后神经干细胞因子mRNA表达的影响.方法 成年雄性SD大鼠63只,采用线栓法建立右侧大脑中动脉缺血再灌注模型,缺血时间为2 h后再灌注.应用随机数字表进行完全随机化的分组,分为治疗组(自造模当日开始,静脉注射牛磺酸80mg/kg体重,持续10 min,1次/d,连用7 d)和对照组(静脉注射0.9%氯化钠注射液1 ml,其余相同)各30只,每组再按同样的随机方法分为再灌注2h、24h、3 d、7d、14d组(每组6只),另取3只为假手术组.应用原位杂交检测脑缺血再灌注后神经干细胞因子(stem cell factor,SCF)mRNA的表达.结果 假手术组SCF mRNA在皮质、纹状体和室旁区有微弱表达.对照组缺血侧SCF mRNA的表达,皮质除2 h以外、纹状体除2 h以外、室旁区除2 h、14 d以外各时间点均高于假手术组(t=2.16～25.19,P＜0.05).治疗组SCF mRNA表达较对照组在皮质、纹状体及室旁区均显著升高,(t=5.19～26.17,P＜0.05).结论 牛磺酸可促进大鼠局灶性脑缺血再灌注后SCF mRNA的表达.
目的 觀察牛磺痠對大鼠缺血性腦損傷後神經榦細胞因子mRNA錶達的影響.方法 成年雄性SD大鼠63隻,採用線栓法建立右側大腦中動脈缺血再灌註模型,缺血時間為2 h後再灌註.應用隨機數字錶進行完全隨機化的分組,分為治療組(自造模噹日開始,靜脈註射牛磺痠80mg/kg體重,持續10 min,1次/d,連用7 d)和對照組(靜脈註射0.9%氯化鈉註射液1 ml,其餘相同)各30隻,每組再按同樣的隨機方法分為再灌註2h、24h、3 d、7d、14d組(每組6隻),另取3隻為假手術組.應用原位雜交檢測腦缺血再灌註後神經榦細胞因子(stem cell factor,SCF)mRNA的錶達.結果 假手術組SCF mRNA在皮質、紋狀體和室徬區有微弱錶達.對照組缺血側SCF mRNA的錶達,皮質除2 h以外、紋狀體除2 h以外、室徬區除2 h、14 d以外各時間點均高于假手術組(t=2.16～25.19,P＜0.05).治療組SCF mRNA錶達較對照組在皮質、紋狀體及室徬區均顯著升高,(t=5.19～26.17,P＜0.05).結論 牛磺痠可促進大鼠跼竈性腦缺血再灌註後SCF mRNA的錶達.
목적 관찰우광산대대서결혈성뇌손상후신경간세포인자mRNA표체적영향.방법 성년웅성SD대서63지,채용선전법건립우측대뇌중동맥결혈재관주모형,결혈시간위2 h후재관주.응용수궤수자표진행완전수궤화적분조,분위치료조(자조모당일개시,정맥주사우광산80mg/kg체중,지속10 min,1차/d,련용7 d)화대조조(정맥주사0.9%록화납주사액1 ml,기여상동)각30지,매조재안동양적수궤방법분위재관주2h、24h、3 d、7d、14d조(매조6지),령취3지위가수술조.응용원위잡교검측뇌결혈재관주후신경간세포인자(stem cell factor,SCF)mRNA적표체.결과 가수술조SCF mRNA재피질、문상체화실방구유미약표체.대조조결혈측SCF mRNA적표체,피질제2 h이외、문상체제2 h이외、실방구제2 h、14 d이외각시간점균고우가수술조(t=2.16～25.19,P＜0.05).치료조SCF mRNA표체교대조조재피질、문상체급실방구균현저승고,(t=5.19～26.17,P＜0.05).결론 우광산가촉진대서국조성뇌결혈재관주후SCF mRNA적표체.
Objective To investigate the effects of taurine on the expression of stem cell factor mRNA of the focal cerebral ischemia in rat.Methods 63 adult male rats were randomly divided into taurine-treated group (Taurine, 80 mg/kg, began at the day of MCAO and continuously intravenous injection for 10 min, daily for 7 days) and controlled group (0.9% normal saline, 1 ml, continuously intravenous injection for 10 min daily for 7 days). The rats received right middle cerebral artery occlusion at 2h and different hours of reperfusion. Expression of SCF was detected by in situ hybridization in the rats subjected to2h,24h,3d,7d, 14 d of reperfusion and sham-operated group (n=3). Results Low level SCF mRNA expressions were found in cortex, striatum and extraventricular zone in sham-operated group. The expression of SCF mRNA in ischemic hemisphere of controlled group increased markedly compared with sham-operated group (t=2.16~25.19, P＜ 0.05), besides that of 2 h in cortex, 2 h in striatum, 2h and 14d in extraventricular zone. SCF mRNA expression in the treatment group increased markedly in cortex,striatum and extraventricular zone at 3d and 7d(t=5.19~ 26.17,P＜0.05). Conclusion Taurine increased the SCF mRNA expression in MCAO rats, therefore it might promote the creation of neural stem cell.
