CAJ | 학술논문

目的评价吸入不同浓度异氟醚对老龄大鼠海马细胞色素c(Cyt c)表达的影响.方法健康雄性老龄SD大鼠63只,月龄20月,体重500～600 g,随机分为3组(n=21),对照组(C组):吸入30%氧气2 h;0.75%异氟醚组(L组):吸入0.75%异氟醚和30%氧气的混合气体2 h;1.5%异氟醚组(I2组):吸入1.5%异氟醚和30%氧气的混合气体2 h.分别于麻醉30 min、1和2 h时,各组取5只大鼠,取动脉血样行血气分析.于麻醉结束后24 h时,各组取8只大鼠,测定认知功能;取8只大鼠,分别采用免疫组化法和Western blot法测定海马Cyt c 的表达水平.结果与C组比较,Ⅰ1组和Ⅰ2组逃避潜伏期延长,原平台象限停留时间缩短,穿越原平台次数减少,海马Cyt c表达上调(P＜0.05);与Ⅰ1组比较,Ⅰ2组逃避潜伏期、原平台象限停留时间缩短和穿越原平台次数差异无统计学意义(P＞0.05),海马Cyt c表达上调(P＜0.05).结论吸入异氟醚可通过上调海马Cyt c的表达导致老龄大鼠认知功能障碍.
목적 평개흡입불동농도이불미대노령대서해마세포색소c(Cyt c)표체적영향.방법 건강웅성노령SD대서63지,월령20월,체중500～600 g,수궤분위3조(n=21),대조조(C조):흡입30%양기2 h;0.75%이불미조(L조):흡입0.75%이불미화30%양기적혼합기체2 h;1.5%이불미조(I2조):흡입1.5%이불미화30%양기적혼합기체2 h.분별우마취30 min、1화2 h시,각조취5지대서,취동맥혈양행혈기분석.우마취결속후24 h시,각조취8지대서,측정인지공능;취8지대서,분별채용면역조화법화Western blot법측정해마Cyt c 적표체수평.결과 여C조비교,Ⅰ1조화Ⅰ2조도피잠복기연장,원평태상한정류시간축단,천월원평태차수감소,해마Cyt c표체상조(P＜0.05);여Ⅰ1조비교,Ⅰ2조도피잠복기、원평태상한정류시간축단화천월원평태차수차이무통계학의의(P＞0.05),해마Cyt c표체상조(P＜0.05).결론 흡입이불미가통과상조해마Cyt c적표체도치노령대서인지공능장애.
Objective To investigate the effects of inhalation of different concentrations of isoflurane on the expression of cytochrome c ( Cyt c) in hippocampus in aged rats.Methods Sixty-three aged male SD rats (20 months) weighing 500-600 g were randomly divided into 3 groups(n=21each):control group inhaling 30%O2 for 2h (group C) and 2 isoflurane groups anesthetized with 0.75 % and 1.5 % isoflurane in 30 % O2 for 2 h respectively (group Ⅰ1 and Ⅰ2 ).Arterial blood samples were obtained from 5 rats at 30 min, 1 and 2 h of anesthesia for blood gas analysis. Eight animals were killed at 24 h after anesthesia in each group.Their hippocampi were immediately removed for determination of Gyt c expression by immuno-histochemistry and Western blot analysis.Cognitive function was assessed by Morris water maze test the day before experiment and once a day for 6 consecutive days starting from the 1st postoperative day.Results The Cyt c expression in hippocampus was significantly increased in Ⅰ1 and Ⅰ2 groups in a concentration-dependent manner as compared with group C.The escape latency was significantly prolonged and the frequency of crossing the original platform and the duration of staying at the original platform quadrant were decreased in group Ⅰ1 and Ⅰ2 compared with group C.Conclusion Inhalation of isoflurane anesthesia can decrease cognitive function through up-regulating the Gyt c expression in hippocampus in aged rats.