中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2011年
9期
951-954
,共4页
急性肺损伤%姜黄素%组织转化生长因子-β1
急性肺損傷%薑黃素%組織轉化生長因子-β1
급성폐손상%강황소%조직전화생장인자-β1
Acute lung injury%Curcumin%Transforming growth factor-β1
目的 观察姜黄素对博莱霉素致急性肺损伤小鼠肺组织转化生长因子-β1(TGF-β1)表达的影响。方法 昆明雄性小鼠60只,随机(随机数字法)分为假手术组,模型组,和姜黄素高、中、低剂量组,每组12只。除假手术组外其余各组小鼠气管内一次性滴注盐酸博莱霉素,假手术组一次性滴注等体积的生理盐水。造模后24h开始给药,持续到处死动物的前1d。姜黄素高、中、低剂量组给药剂量分别为200,100,50 mg·kg-1·d-1,假手术组、模型组分别灌服等体积的生理盐水。各组动物分别于给药后第3天和第7天随机处死6只,取肺组织进行HE染色,观察肺组织形态学改变;检测肺湿干比值;同时进行免疫组化显色,检测各组小鼠肺组织中转化生长因子-β1的表达水平。数据以均数±标准差((x)±s)表示。采用SPSS 11.5统计软件进行分析。多样本均数的比较采用单因素方差分析。结果 假手术组小鼠未见明显形态学异常,肺泡内未见水肿液及炎性细胞;模型组肺组织毛细血管明显扩张充血,肺泡间隔增宽,肺泡腔内可见水肿液及大量炎性细胞浸润;姜黄素高、中、低剂量组也可见不同程度的病理改变,但与模型组比肺损伤程度明显减轻。模型组肺湿干比值较假手术组明显升高(P＜0.01),但姜黄索各剂量组湿干比值较模型组明显降低(P＜0.01)。假手术组肺组织TGF-β1呈低水平表达,模型组肺组织TGF-β1表达显著高于假手术组(P＜0.01)。姜黄素高、中、低剂量组肺组织TGF-β1蛋白表达较模型组明显降低(P＜0.01),但仍高于假手术组(P＜0.01)。结论 姜黄素可抑制肺损伤小鼠肺组织中TGF-β1的表达,减轻肺损伤的程度,对急性肺损伤具有一定的保护作用,其保护机制可能是通过抑制肺组织中TGF-β1的表达来实现的。
目的 觀察薑黃素對博萊黴素緻急性肺損傷小鼠肺組織轉化生長因子-β1(TGF-β1)錶達的影響。方法 昆明雄性小鼠60隻,隨機(隨機數字法)分為假手術組,模型組,和薑黃素高、中、低劑量組,每組12隻。除假手術組外其餘各組小鼠氣管內一次性滴註鹽痠博萊黴素,假手術組一次性滴註等體積的生理鹽水。造模後24h開始給藥,持續到處死動物的前1d。薑黃素高、中、低劑量組給藥劑量分彆為200,100,50 mg·kg-1·d-1,假手術組、模型組分彆灌服等體積的生理鹽水。各組動物分彆于給藥後第3天和第7天隨機處死6隻,取肺組織進行HE染色,觀察肺組織形態學改變;檢測肺濕榦比值;同時進行免疫組化顯色,檢測各組小鼠肺組織中轉化生長因子-β1的錶達水平。數據以均數±標準差((x)±s)錶示。採用SPSS 11.5統計軟件進行分析。多樣本均數的比較採用單因素方差分析。結果 假手術組小鼠未見明顯形態學異常,肺泡內未見水腫液及炎性細胞;模型組肺組織毛細血管明顯擴張充血,肺泡間隔增寬,肺泡腔內可見水腫液及大量炎性細胞浸潤;薑黃素高、中、低劑量組也可見不同程度的病理改變,但與模型組比肺損傷程度明顯減輕。模型組肺濕榦比值較假手術組明顯升高(P＜0.01),但薑黃索各劑量組濕榦比值較模型組明顯降低(P＜0.01)。假手術組肺組織TGF-β1呈低水平錶達,模型組肺組織TGF-β1錶達顯著高于假手術組(P＜0.01)。薑黃素高、中、低劑量組肺組織TGF-β1蛋白錶達較模型組明顯降低(P＜0.01),但仍高于假手術組(P＜0.01)。結論 薑黃素可抑製肺損傷小鼠肺組織中TGF-β1的錶達,減輕肺損傷的程度,對急性肺損傷具有一定的保護作用,其保護機製可能是通過抑製肺組織中TGF-β1的錶達來實現的。
목적 관찰강황소대박래매소치급성폐손상소서폐조직전화생장인자-β1(TGF-β1)표체적영향。방법 곤명웅성소서60지,수궤(수궤수자법)분위가수술조,모형조,화강황소고、중、저제량조,매조12지。제가수술조외기여각조소서기관내일차성적주염산박래매소,가수술조일차성적주등체적적생리염수。조모후24h개시급약,지속도처사동물적전1d。강황소고、중、저제량조급약제량분별위200,100,50 mg·kg-1·d-1,가수술조、모형조분별관복등체적적생리염수。각조동물분별우급약후제3천화제7천수궤처사6지,취폐조직진행HE염색,관찰폐조직형태학개변;검측폐습간비치;동시진행면역조화현색,검측각조소서폐조직중전화생장인자-β1적표체수평。수거이균수±표준차((x)±s)표시。채용SPSS 11.5통계연건진행분석。다양본균수적비교채용단인소방차분석。결과 가수술조소서미견명현형태학이상,폐포내미견수종액급염성세포;모형조폐조직모세혈관명현확장충혈,폐포간격증관,폐포강내가견수종액급대량염성세포침윤;강황소고、중、저제량조야가견불동정도적병리개변,단여모형조비폐손상정도명현감경。모형조폐습간비치교가수술조명현승고(P＜0.01),단강황색각제량조습간비치교모형조명현강저(P＜0.01)。가수술조폐조직TGF-β1정저수평표체,모형조폐조직TGF-β1표체현저고우가수술조(P＜0.01)。강황소고、중、저제량조폐조직TGF-β1단백표체교모형조명현강저(P＜0.01),단잉고우가수술조(P＜0.01)。결론 강황소가억제폐손상소서폐조직중TGF-β1적표체,감경폐손상적정도,대급성폐손상구유일정적보호작용,기보호궤제가능시통과억제폐조직중TGF-β1적표체래실현적。
Objective To observe the level of transforming growth factor-β1 ( TGF-31 ) in lung tissue of mice with acute lung injury induced by bleomycin. Methods Sixty Kunming male mice were randomly (random number) divided into sham operation group, model group, curcumin high dose group,medium dose group and low dose group (n =12 in each group). The models of acute lung injury were made by intratracheal instillation of bleomycin for once in the mice of model group and curcumin treated groups,and equal volume of saline was used in mice of sham operation group instead of bleomycin. Curcumin was given in doses of 200 mg· kg-1 · d-1 , 100 mg· kg-1 · d-1and 50 mg · kg-1 · d-1 to the mice of high dose group, medium dose group and low dose group, respectively 24 hours after modeling until the day before sacrifice, and the mice of sham operation group and model group were fed with equal volume of saline instead. Six mice of each group were sacrificed on the 3rd and 7th day separately for detecting lung wet to dry ratio (W/D), pathological changes of lung tissue stained with HE, and level of the transforming growth factor-β1 in the lung tissues stained with immunohistochemistry in mice of each group. Data were analyzed to express as mean ± standard deviation. -PSS11.5 statistical package was used for analysis. The comparison of the mean diversity carried out by using single factor analysis of variance. Results Morphological changes in lung tissue observed under light microscope showed that obvious morphological abnormalities,alveolar edema fluid and inflammatory cells were not found in mice of sham operation group, and lung capillaries dilated, widened alveolar septum, edema fluid and inflammatory cells infiltration in the alveolar in lung tissue were seen in mice of model group, and different degrees of pathological changes were found in mice of three curcumin treated groups, but those changes were less severe than those in mice of model group. Lung wet to dry ratio (W/D) was significantly higher in mice of model group than that in mice of sham group ( P ＜ 0. 01 ), but that in mice of three curcumin treated groups was significantly lower than that in mice of model group ( P ＜ 0. 01 ). Immunohistochemistry showed low level of TGF-β1 in lung tissue of mice in sham operation group, and the level of TGF-β1 in lung tissue of mice in model group was significantly higher than that in sham operation group ( P ＜ 0. 01 ). The level of TGF-β1 in lung tissue of mice in three curcumin treated groups was significantly lower than that in mice of model group ( P ＜ 0. 01 ),but that was still higher than that in sham group ( P ＜ 0. 01 ). Conclusions Curcumin can inhibit the TGF-β1 in lung tissue and play a protective effect on acute lung injury induced by bleomycin, reducing the degree of lung injury. The protective mechanism may be associated with the inhibition of TGF-β1 in lung tissue.
