中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2011年
10期
929-933
,共5页
裴晶晶%郭帅%张翠丽%于丽华%朱振平%谢克勤%赵秀兰
裴晶晶%郭帥%張翠麗%于麗華%硃振平%謝剋勤%趙秀蘭
배정정%곽수%장취려%우려화%주진평%사극근%조수란
甲硫氨酸%大鼠%纤溶酶原激活剂%纤溶酶原激活物抑制物1%茶多酚
甲硫氨痠%大鼠%纖溶酶原激活劑%纖溶酶原激活物抑製物1%茶多酚
갑류안산%대서%섬용매원격활제%섬용매원격활물억제물1%다다분
Methionine%Rats%Plasminogen activators%Plasminogen activator inhibitor 1%Tea polyphenols
目的 研究茶多酚( tea polyphenols，TP)对蛋氨酸诱导大鼠纤维蛋白溶解功能损伤的保护作用。方法 取成年雄性Wistar大鼠50只，按体重分层随机分为对照组、模型组，以及TP低、中和高剂量组。对照组喂饲正常基础饲料，模型组及TP组均喂饲3％高蛋氨酸饲料，同时TP低、中、高剂量组每天经灌胃分别给予50、100和200 mg/kg的TP，灌胃体积均为0.5ml/100 9,对照组和模型组给予等体积蒸馏水。8周后断头处死大鼠，采用免疫组织化学链霉亲和素-生物素-过氧化物酶复合物（strept-avidin-biotin complex，SABC）法测定大鼠主动脉弓组织型纤溶酶原激活物（tissue-type plasminogen activator,t-PA）和纤溶酶原激活物抑制剂-1（type-1 plasminogen activator inhibitor，PAI-1）蛋白表达结果；ELISA法测定血浆中t-PA和PAI-1含量；RT-PCR法测定胸主动脉t-PA和PAI-1 mRNA水平。结果 实验结束后，对照组、模型组及TP低、中、高剂量组主动脉弓中t-PA蛋白表达量分别为133.03±10.14、95.46±11.08、111.97±11.91、130.23±10.80、139.39±9.41 (F= 14.15，P＜0.01)，PAI-1蛋白表达量分别为90.91±8.67、166.76±12.18、139.63±12.71、134.66±13.19、109.49±10.82(F=31.44，P＜0.01)；血浆中tPA含量分别为(10.69±1.26)、(6.13±0.92)、(8.56±1.19)、(9.69±0.92)、(11.97±1.08) ng/ml(F=41.98,P＜0.01)，PAI-1含量分别为(6.31±0.81)、(16.98±1.27)、(11.39±0.82)、(8.46±0.67)、(8.08±0.91) ng/ml(F= 207.74,P＜0.01);t-PA mRNA水平分别为1.12±0.02、0.75±0.14、1.01±0.09、0.95±0.08、1.05±0.13(F=5.77,P＜0.05)；PAI-1 mRNA水平分别为1.25±0.11、1.74±0.06、1.23±0.05、1.09±0.14、1.23±0.04(F=23.56，P＜0.01)。结论 TP能够调节t-PA和PAI-1的转录及蛋白水平，维持t-PA和PAI-1的平衡状态，修复高蛋氨酸饮食诱导的大鼠纤维蛋白溶解功能损伤。
目的 研究茶多酚( tea polyphenols，TP)對蛋氨痠誘導大鼠纖維蛋白溶解功能損傷的保護作用。方法 取成年雄性Wistar大鼠50隻，按體重分層隨機分為對照組、模型組，以及TP低、中和高劑量組。對照組餵飼正常基礎飼料，模型組及TP組均餵飼3％高蛋氨痠飼料，同時TP低、中、高劑量組每天經灌胃分彆給予50、100和200 mg/kg的TP，灌胃體積均為0.5ml/100 9,對照組和模型組給予等體積蒸餾水。8週後斷頭處死大鼠，採用免疫組織化學鏈黴親和素-生物素-過氧化物酶複閤物（strept-avidin-biotin complex，SABC）法測定大鼠主動脈弓組織型纖溶酶原激活物（tissue-type plasminogen activator,t-PA）和纖溶酶原激活物抑製劑-1（type-1 plasminogen activator inhibitor，PAI-1）蛋白錶達結果；ELISA法測定血漿中t-PA和PAI-1含量；RT-PCR法測定胸主動脈t-PA和PAI-1 mRNA水平。結果 實驗結束後，對照組、模型組及TP低、中、高劑量組主動脈弓中t-PA蛋白錶達量分彆為133.03±10.14、95.46±11.08、111.97±11.91、130.23±10.80、139.39±9.41 (F= 14.15，P＜0.01)，PAI-1蛋白錶達量分彆為90.91±8.67、166.76±12.18、139.63±12.71、134.66±13.19、109.49±10.82(F=31.44，P＜0.01)；血漿中tPA含量分彆為(10.69±1.26)、(6.13±0.92)、(8.56±1.19)、(9.69±0.92)、(11.97±1.08) ng/ml(F=41.98,P＜0.01)，PAI-1含量分彆為(6.31±0.81)、(16.98±1.27)、(11.39±0.82)、(8.46±0.67)、(8.08±0.91) ng/ml(F= 207.74,P＜0.01);t-PA mRNA水平分彆為1.12±0.02、0.75±0.14、1.01±0.09、0.95±0.08、1.05±0.13(F=5.77,P＜0.05)；PAI-1 mRNA水平分彆為1.25±0.11、1.74±0.06、1.23±0.05、1.09±0.14、1.23±0.04(F=23.56，P＜0.01)。結論 TP能夠調節t-PA和PAI-1的轉錄及蛋白水平，維持t-PA和PAI-1的平衡狀態，脩複高蛋氨痠飲食誘導的大鼠纖維蛋白溶解功能損傷。
목적 연구다다분( tea polyphenols，TP)대단안산유도대서섬유단백용해공능손상적보호작용。방법 취성년웅성Wistar대서50지，안체중분층수궤분위대조조、모형조，이급TP저、중화고제량조。대조조위사정상기출사료，모형조급TP조균위사3％고단안산사료，동시TP저、중、고제량조매천경관위분별급여50、100화200 mg/kg적TP，관위체적균위0.5ml/100 9,대조조화모형조급여등체적증류수。8주후단두처사대서，채용면역조직화학련매친화소-생물소-과양화물매복합물（strept-avidin-biotin complex，SABC）법측정대서주동맥궁조직형섬용매원격활물（tissue-type plasminogen activator,t-PA）화섬용매원격활물억제제-1（type-1 plasminogen activator inhibitor，PAI-1）단백표체결과；ELISA법측정혈장중t-PA화PAI-1함량；RT-PCR법측정흉주동맥t-PA화PAI-1 mRNA수평。결과 실험결속후，대조조、모형조급TP저、중、고제량조주동맥궁중t-PA단백표체량분별위133.03±10.14、95.46±11.08、111.97±11.91、130.23±10.80、139.39±9.41 (F= 14.15，P＜0.01)，PAI-1단백표체량분별위90.91±8.67、166.76±12.18、139.63±12.71、134.66±13.19、109.49±10.82(F=31.44，P＜0.01)；혈장중tPA함량분별위(10.69±1.26)、(6.13±0.92)、(8.56±1.19)、(9.69±0.92)、(11.97±1.08) ng/ml(F=41.98,P＜0.01)，PAI-1함량분별위(6.31±0.81)、(16.98±1.27)、(11.39±0.82)、(8.46±0.67)、(8.08±0.91) ng/ml(F= 207.74,P＜0.01);t-PA mRNA수평분별위1.12±0.02、0.75±0.14、1.01±0.09、0.95±0.08、1.05±0.13(F=5.77,P＜0.05)；PAI-1 mRNA수평분별위1.25±0.11、1.74±0.06、1.23±0.05、1.09±0.14、1.23±0.04(F=23.56，P＜0.01)。결론 TP능구조절t-PA화PAI-1적전록급단백수평，유지t-PA화PAI-1적평형상태，수복고단안산음식유도적대서섬유단백용해공능손상。
Objective To study the protective impact of tea polyphenols (TP) on the injury of fibrinolytic functions induced by high-methionine dietary in rats. Methods50 male Wistar rats were divided by stratified based on body weight into 5 groups with 10 in each group: namely control group,model group,low-dose TP group,medium-dose TP group and high-dose TP group. The rats in model group and TP groups were fed with 3％ methionine dietary, control group rats with routine diet. In addition, rats in lowdose,medium-dose and high-dose TP groups were treated with TP at 50,100 and 200 mg/kg dosage respectively by gavages every day,control group and model group rats were given with same amount distilled water. The animals were sacrificed after 8 weeks. The levels of tissue-type plasminogen activator (t-PA) and type-1 plasminogen activator inhibitor( PAI-1 ) in plasma were determined by ELISA assays,mRNA levels of t-PA and PAI-1 in aortic arch were detected by RT-PCR, t-PA and PAI-1 expression in aortic arch were detected by immunohistochemistry strept-avidin-biotin complex ( SABC ). Results After experiment, the t-PA expression of aortic arch in control group, model group, low-dose TP group, medium-dose TP group and high-dose TP group were 133.03 ± 10. 14,95.46 ± 11.08,111.97 ± 11.91,130. 23 ± 10. 80,139. 39 ±9. 41(F = 14. 15, P ＜ 0. 01 ), respectively, and the PAI-1 expression were 90. 91 ± 8.67,166. 76 ± 12. 18,139. 63±12. 71,134. 66 ± 13. 19, 1 09.49 ± 10. 82 ( F = 31.44, P ＜ 0. 01 ). The t-PA concentration of plasma were ( 1 0. 69 ± 1. 26 ), ( 6. 13 ± 0. 92 ), ( 8. 56 ± 1.19), (9. 69 ± 0. 92 ), ( 11.97 ± 1.08 ) ng/ml, respectively ( F =41.98, P ＜ 0. 01 ), and the PAI-1 concentration of plasma were ( 6. 31 ± 0. 81 ), ( 16. 98 ± 1.27 ),( 11.39 ± 0. 82), ( 8.46 ± 0. 67 ), ( 8.08 ± 0. 91 ) ng/ml, respectively ( F = 207. 74, P ＜ 0. 01 ). The mRNA levels of t-PA in aortic arch were 1.12 ±0.02,0.75 ±0. 14,1.01 ±0.09,0.95 ±0.08,1.05 ±0. 13(F= 5. 77, P ＜ 0. 05 ), and the mRNA levels of PAI-1 in aortic arch were 1.25 ± 0. 11,1.74 ± 0. 06,1.23 ± 0. 05,1.09 ± 0. 14,1.23 ± 0. 04 ( F = 23.56, P ＜ 0. 01 ). Conclusion The results indicate that TP seems to have regulatary function on transcription and protein levels of t-PA and PAI-1, in addition to maintaining the balance between PAI-1 and t-PA and healing the injury of fibrinolytic functions in rats induced by highmethionine dietary.
