CAJ | 학술논문

目的构建具活性细胞成分的激光微孔化猪脱细胞真皮基质(LPADM),并通过Ⅰ期复合自体刃厚皮片移植观察其在修复SD大鼠全层皮肤缺损中的效果.方法设LPADM组与无孔PADM组,将同种异体成纤维细胞(fibroblast,FB)种植于LPADM上培养,另设仅用FB培养液培养的FB为对照组,采用双抗体夹心ABC-ELISA法测定各组FB细胞活性因子白细胞介素-10(IL-10)、白细胞介素-6(IL-6)、β1转化生长因子(TGF-β1)、层粘连蛋白(LN)、血管内皮细胞生长因子(VEGF)的表达.制作SD大鼠全层皮肤缺损伤口模型,将具活性的LPADM复合自体刃厚皮片Ⅰ期移植,记录其生长情况,定期活检,并进行组织学观察.结果 LPADM组、PADM组、对照组各组各时相点各指标吸光度值比较,差异均无统计学意义(F为0.050～1.763,P＞0.05).移植的LPADM中可见生长良好的FB.移植术后3周,创面愈合良好,未见明显排斥反应.术后1个月,创面瘢痕轻,复合皮肤可提捏.结论用活性LPADM复合自体刃厚皮片Ⅰ期移植修复SD大鼠全层皮肤缺损,可有效修复创面,并具有较高的生物安全性.
목적 구건구활성세포성분적격광미공화저탈세포진피기질(LPADM),병통과Ⅰ기복합자체인후피편이식관찰기재수복SD대서전층피부결손중적효과.방법 설LPADM조여무공PADM조,장동충이체성섬유세포(fibroblast,FB)충식우LPADM상배양,령설부용FB배양액배양적FB위대조조,채용쌍항체협심ABC-ELISA법측정각조FB세포활성인자백세포개소-10(IL-10)、백세포개소-6(IL-6)、β1전화생장인자(TGF-β1)、층점련단백(LN)、혈관내피세포생장인자(VEGF)적표체.제작SD대서전층피부결손상구모형,장구활성적LPADM복합자체인후피편Ⅰ기이식,기록기생장정황,정기활검,병진행조직학관찰.결과 LPADM조、PADM조、대조조각조각시상점각지표흡광도치비교,차이균무통계학의의(F위0.050～1.763,P＞0.05).이식적LPADM중가견생장량호적FB.이식술후3주,창면유합량호,미견명현배척반응.술후1개월,창면반흔경,복합피부가제날.결론 용활성LPADM복합자체인후피편Ⅰ기이식수복SD대서전층피부결손,가유효수복창면,병구유교고적생물안전성.
Objective To prepare a new type of micropore porcine acellular dermis matrix with the aid of laser (LPADM),and to validate the healing effect of the LPADM through the phrase Ⅰ composite transplanting on the back of the full- thickness skin defects in SD rats.Methods In vitro,the allogeneic fibroblasts were separately cultured with the LPADM (LPADM group) or the non-pore PADM (non-pore LPADM group),while fibroblasts cultured by pure medium were used as control.After culture of 1 day,3 days and 5 days,the contents of IL-10,IL-6,TGF-β1,LN,VEGF expressed by fibroblasts were determined by double-antibody sandwich ELISA method.In vivo,the phrase Ⅰ transplantations of LPADM graft with split-thickness autologous skin were carried on the backs of the full-thickness cutaneous defects of SD rats.The healing condition was observed and analyzed by histological tests.Results The differences of the absorbance value between the LPADMgroup,PADM group and control group in each day were not statistically significant (F=0.050-1.763,P＞0.05).The transplantation of LPADM graft with split-thickness autologous skin graft resulted in high rate of surviving without signs of rejection 3 weeks later.After 1-month of transplantation,the regenerated skin was well enough to be lifted without any serious scars.Conclusions The phrase Ⅰ transplantation of LPADM graft with split-thickness autologous skin graft can accelerate the healing process of full-thickness skin wounds with high biological safety.