CAJ | 학술논문

通过研究不同年龄组大鼠脑组织中微管相关蛋白tau的表达特性和磷酸化位点的差异，为阿尔茨海默病（AD）脑组织中tau蛋白的研究提供资料。将大鼠分为胎鼠、新生鼠、成年鼠和老年鼠4组，取各组大鼠的脑组织制成匀浆，用免疫印迹法检测各组tau蛋白的分子量范围及磷酸化状态。结果发现非磷酸化依赖性抗tau抗体R134d的免疫显色结果显示：胎鼠和新生鼠主要含低分子量（14.2～28.8 ku）tau蛋白；成年鼠除在脊索中显示高分子量tau（67.5～96.6 ku）外，大脑灰质和白质中的tau蛋白主要集中在中分子量（28.8～42.7 ku）范围；老年鼠的灰质、白质和脊索中均含高分子量tau蛋白，且在灰质和白质组分中中分子量tau的含量比成年组显著增高。Tau-1的显色性质与R134 d相似。PHF-1和M4只在胎鼠和新生鼠显带。认为大鼠神经系统tau蛋白的表达性质及其磷酸化状态随年龄增长而显著不同。
통과연구불동년령조대서뇌조직중미관상관단백tau적표체특성화린산화위점적차이，위아이자해묵병（AD）뇌조직중tau단백적연구제공자료。장대서분위태서、신생서、성년서화노년서4조，취각조대서적뇌조직제성균장，용면역인적법검측각조tau단백적분자량범위급린산화상태。결과발현비린산화의뢰성항tau항체R134d적면역현색결과현시：태서화신생서주요함저분자량（14.2～28.8 ku）tau단백；성년서제재척색중현시고분자량tau（67.5～96.6 ku）외，대뇌회질화백질중적tau단백주요집중재중분자량（28.8～42.7 ku）범위；노년서적회질、백질화척색중균함고분자량tau단백，차재회질화백질조분중중분자량tau적함량비성년조현저증고。Tau-1적현색성질여R134 d상사。PHF-1화M4지재태서화신생서현대。인위대서신경계통tau단백적표체성질급기린산화상태수년령증장이현저불동。
To study the difference in expression and phosphorylation of tau in normal rat neural system and therefore to provide information for Alzheimer disease (AD) studies on tau protein in brain，the brain gray matter，white matter or spinal cord from fetal，neonatal，adult and aged rats were isolated and homogenized，the supernatants were used for the analysis of expression and phosphorylation of tau by Western blotting. The results showed that in the fetal and neonatal rats only low molecular weight tau ranged from 14.2 to 28.8 ku were detected. On the other hand，middle (28.8—42.7 ku) and high（67.5—96.6 ku）molecular weight tau were seen in the adult and aged rats. The monoclonal antibody tau-1 reacted with all the isoforms of tau detected by R-134d that was supposed to bind to total tau. M4 and PHF-1 epitop phosphorylated tau were only determined in the fetal and neonatal，but not in the adult and aged rat neural system. The expression and phosphorylation of tau from central neural system were developmentally regulated，and phosphorylation of tau at M4 and PHF-1 epitop was not detected in normal condition.