药学学报
藥學學報
약학학보
ACTA PHARMACEUTICA SINICA
2004年
10期
782-786
,共5页
王佩%任兴昌%俞进%林宜%吴锡铭
王珮%任興昌%俞進%林宜%吳錫銘
왕패%임흥창%유진%림의%오석명
N,N′-二乙酰-L-胱氨酸%暴发性肝衰竭%半乳糖胺%脂多糖%免疫调节剂%T细胞亚群
N,N′-二乙酰-L-胱氨痠%暴髮性肝衰竭%半乳糖胺%脂多糖%免疫調節劑%T細胞亞群
N,N′-이을선-L-광안산%폭발성간쇠갈%반유당알%지다당%면역조절제%T세포아군
N,N'-diacetyl-L-cystine%fulminant heptic failure%galactosamine%lipopolysaccharide%immunomodulatory agent%T cell subsets
目的研究N,N′-二乙酰-L-胱氨酸(DiNAC)对免疫性肝衰竭的治疗作用.方法观察DiNAC对Balb/C小鼠由半乳糖胺联用脂多糖引起免疫性肝衰竭的作用.半乳糖胺/脂多糖攻击6 h后,小鼠血清ALT,AST和外周血T细胞亚群分别用全自动生化仪、流式细胞仪测定,并用光镜观察肝组织病理切片,统计半乳糖胺/脂多糖攻击24 h后的小鼠存活率.结果给肝衰竭小鼠ip DiNAC(50,200,800mg·kg-1),能明显阻止小鼠血清ALT和AST活力增高,使肝组织损害减轻及提高小鼠存活率,并呈剂量依赖关系;DiNAC能增强免疫性肝衰竭小鼠外周血CD4+,CD8+,Th1和Th2 T淋巴细胞的增殖分化.结论DiNAC对免疫性肝衰竭动物有明显的治疗作用,这一作用与其免疫调节有关.
目的研究N,N′-二乙酰-L-胱氨痠(DiNAC)對免疫性肝衰竭的治療作用.方法觀察DiNAC對Balb/C小鼠由半乳糖胺聯用脂多糖引起免疫性肝衰竭的作用.半乳糖胺/脂多糖攻擊6 h後,小鼠血清ALT,AST和外週血T細胞亞群分彆用全自動生化儀、流式細胞儀測定,併用光鏡觀察肝組織病理切片,統計半乳糖胺/脂多糖攻擊24 h後的小鼠存活率.結果給肝衰竭小鼠ip DiNAC(50,200,800mg·kg-1),能明顯阻止小鼠血清ALT和AST活力增高,使肝組織損害減輕及提高小鼠存活率,併呈劑量依賴關繫;DiNAC能增彊免疫性肝衰竭小鼠外週血CD4+,CD8+,Th1和Th2 T淋巴細胞的增殖分化.結論DiNAC對免疫性肝衰竭動物有明顯的治療作用,這一作用與其免疫調節有關.
목적연구N,N′-이을선-L-광안산(DiNAC)대면역성간쇠갈적치료작용.방법관찰DiNAC대Balb/C소서유반유당알련용지다당인기면역성간쇠갈적작용.반유당알/지다당공격6 h후,소서혈청ALT,AST화외주혈T세포아군분별용전자동생화의、류식세포의측정,병용광경관찰간조직병리절편,통계반유당알/지다당공격24 h후적소서존활솔.결과급간쇠갈소서ip DiNAC(50,200,800mg·kg-1),능명현조지소서혈청ALT화AST활력증고,사간조직손해감경급제고소서존활솔,병정제량의뢰관계;DiNAC능증강면역성간쇠갈소서외주혈CD4+,CD8+,Th1화Th2 T림파세포적증식분화.결론DiNAC대면역성간쇠갈동물유명현적치료작용,저일작용여기면역조절유관.
Aim To study the therapeutic effects of N, N'-diacetyl-L-cystine (DiNAC) on immunological liver failure. Methods Serum ALT, AST and T cell subsets in peripheral blood of the experimental animals during the trial period were analyzed by an automatic serum analyzer and a flow cytometer, respectively. The sectioned liver specimens were examined under a light microscope. And 24 h after the injection of Gal/LPS, the survival rate of rats was calculated. Results DiNAC (50, 200, 800 and differentiation of T cell subsets ( CD4 + , CD8 + and Th1, Th2), and improved all the histopathological features. In mice of fulminant hepatic failure (FHF), the survival time significantly prolonged and the survival rate increased 24 h after ip DiNAC. These effects were obviously dose-dependent. Conclusion DiNAC on mice with FHF has an inhibitory action which is related to immune mechanism.
