CAJ | 학술논문

目的探讨应用化疗+G-CSF方法动员外周血干细胞时,恶性血液病患者的骨髓及外周血CD34+细胞和T细胞亚群的变化特点.方法 16例拟行自体外周血干细胞移植患者,在应用化疗+G-CSF方法动员外周血干细胞期间,应用流式细胞仪测定骨髓及外周血CD34+和T细胞亚群动态变化情况.结果 1.恶性血液病患者在应用G-CSF前,骨髓中CD34+细胞的初始值为(0.52±0.31)%.应用G-CSF 48 h后,CD34+细胞测定值为(1.22±0.42)%,较前有明显增加(P＜0.001).CD34+细胞的高峰值出现在应用G-CSF 96 h后,为(2.23±0.34)%,较未应用G-CSF时差异显著(P＜0.001).2.恶性血液病患者在应用G-CSF前,外周血CD34+细胞测定值为(0.39±0.27)%.在应用G-CSF 72 h后外周血中CD34+细胞的测定值为(1.29±0.64)%,较前有明显增加(P＜0.001).应用G-CSF96 h后,CD34+细胞的测定值达高峰,为(1.41±0.73)%,较未应用G-CSF前差异显著(P＜0.001).3.无论是否应用G-CSF,恶性血液病患者的T淋巴细胞亚群比例均处于倒置状态,应用G-CSF后随CD34+细胞的逐渐增加,T淋巴细胞亚群变化不明显(P＞0.05).结论恶性血液病患者,在应用化疗+G-CSF动员外周血干细胞时,其骨髓及外周血CD34+细胞和T细胞亚群的动态变化各有其特点.
목적탐토응용화료+G-CSF방법동원외주혈간세포시,악성혈액병환자적골수급외주혈CD34+세포화T세포아군적변화특점.방법 16례의행자체외주혈간세포이식환자,재응용화료+G-CSF방법동원외주혈간세포기간,응용류식세포의측정골수급외주혈CD34+화T세포아군동태변화정황.결과 1.악성혈액병환자재응용G-CSF전,골수중CD34+세포적초시치위(0.52±0.31)%.응용G-CSF 48 h후,CD34+세포측정치위(1.22±0.42)%,교전유명현증가(P＜0.001).CD34+세포적고봉치출현재응용G-CSF 96 h후,위(2.23±0.34)%,교미응용G-CSF시차이현저(P＜0.001).2.악성혈액병환자재응용G-CSF전,외주혈CD34+세포측정치위(0.39±0.27)%.재응용G-CSF 72 h후외주혈중CD34+세포적측정치위(1.29±0.64)%,교전유명현증가(P＜0.001).응용G-CSF96 h후,CD34+세포적측정치체고봉,위(1.41±0.73)%,교미응용G-CSF전차이현저(P＜0.001).3.무론시부응용G-CSF,악성혈액병환자적T림파세포아군비례균처우도치상태,응용G-CSF후수CD34+세포적축점증가,T림파세포아군변화불명현(P＞0.05).결론악성혈액병환자,재응용화료+G-CSF동원외주혈간세포시,기골수급외주혈CD34+세포화T세포아군적동태변화각유기특점.
Objective: To approach what changes happen about CD34 + cells and T lymphocyte subsets after using chemotherapy plus G - CSF with patients. Methods: Flow cytometry was used to detect the changes of the percentages of CD34 + cells and T lymphocyte cell subsets in 16 cases of patients when they were treated with chemotherapy and injected with G - CSF for mobilizing peripheral blood stem cells. Results: 1. Without using G - CSF, the percentages of CD34 + cells in the bone marrow of the patients ranged in (0.52 ± 0.31) %. After Using G - CSF for 48hrs, the percentages ranged in (1.22±0.42)%,obviously increased compared with those of no using G - CSF (P＜0.001).After 96hrs the highest percentages were reached and ranged in (2.23 ± 0.34) % , significant increased compared with those of no using G - CSF (P＜0.001). 2. Without using G - CSF, the percentages of CD34 + cells in peripheral blood of the patients ranged in (0.39 ± 0.27 ) %. After using G - CSF for 72 hours, they ranged in ( 1.29±0.64)%, obviously increased compared with those of no using G- CSF (P＜0.001). After 96 hrs the highest percentages were reached and ranged in (1.41±0.73) %, significant increased compared with those of no using G -CSF (P＜0.001). 3. CD4/CDs of the patients were abnormal (＜1) whether in bone marrow cells or peripheral blood cells. There were no obvious changes of T lymphocyte subsets in the patients except that CD34 + cells increased gradually(P＞0.05) when using G -CSF for some days . Conclusions:The dynamic change of the percentages of CD34 +cells and T lymphocyte subsets in bone marrow or peripheral blood of patients with CR complete remission has itself characteristics after using chemotherapy plus G - CSF for days.