CAJ | 학술논문

[目的] 研究对比体外受精-单精子卵胞浆内注射(IVF-ICSI)周期所获生殖泡期(GV)不成熟卵母细胞以及体外成熟(IVM)周期所获GV期卵母细胞体外培养成熟后的胚胎发育情况.[方法] 163个IVF-ICSI周期中所获987个成熟卵为Ⅰ组,所获GV期卵进行体外培养后成熟的132个卵为Ⅱ组,另有37个IVM周期中所获GV期卵体外培养后成熟的235个卵为Ⅲ组.对3组均进行ICSI,并对其受精率、卵裂率及胚胎发育进行比较.[结果] Ⅰ组的受精率、卵裂率以及优质胚胎率均显著高于Ⅱ组及Ⅲ组(分别为84.9%,98.1%和61.6%;72.0%,90.5%和22.1%;75.3%,94.4%和25.1%).Ⅰ组的胚胎卵裂球数目及胚胎形态评分均显著优于Ⅱ组及Ⅲ组(P ＜ 0.05);Ⅱ组与Ⅲ组相比差异无统计学意义. [结论] 体内成熟卵形成的胚胎形态好于生殖泡期卵体外培养成熟者,IVF-ICSI周期的生殖泡期不成熟卵形成的胚胎形态学上与IVM周期的类似.
[목적] 연구대비체외수정-단정자란포장내주사(IVF-ICSI)주기소획생식포기(GV)불성숙란모세포이급체외성숙(IVM)주기소획GV기란모세포체외배양성숙후적배태발육정황.[방법] 163개IVF-ICSI주기중소획987개성숙란위Ⅰ조,소획GV기란진행체외배양후성숙적132개란위Ⅱ조,령유37개IVM주기중소획GV기란체외배양후성숙적235개란위Ⅲ조.대3조균진행ICSI,병대기수정솔、란렬솔급배태발육진행비교.[결과] Ⅰ조적수정솔、란렬솔이급우질배태솔균현저고우Ⅱ조급Ⅲ조(분별위84.9%,98.1%화61.6%;72.0%,90.5%화22.1%;75.3%,94.4%화25.1%).Ⅰ조적배태란렬구수목급배태형태평분균현저우우Ⅱ조급Ⅲ조(P ＜ 0.05);Ⅱ조여Ⅲ조상비차이무통계학의의. [결론] 체내성숙란형성적배태형태호우생식포기란체외배양성숙자,IVF-ICSI주기적생식포기불성숙란형성적배태형태학상여IVM주기적유사.
[Objective] This study compared outcomes of in vitro maturation (IVM) and in vitro fertilization (IVF) intracytoplasmic sperm injection (ICSI) cycles after IVM of immature germinal vesicle (GV) oocytes.[Methods] ICSI was performed on metaphase II (MII) oocytes retrieved in 163 IVF-ICSI cycles (group I;n ＝ 987) or matured from GV stage oocytes in IVF-ICSI ( group II;n ＝ 132) and 37 IVM cycles ( group III;n ＝ 235).Fertilization and cleavage rates and embryo quality were compared among the three groups.[Results] The fertilization rate,cleavage rate and top quality embryos rate were higher in group I than group II and group III (84.9%,98.1%,and 61.6%;72.0%,90.5% and 22.1%l;75.3%,94.4%,and 25.1%,respectively).Blastomere numbers and morphology scores were highest in group I (P ＜ 0.05),but no significant differences existed between group II and group III.[Conclusion] The morphology of embryos developed from in vivo MII oocytes was superior to those from in vitro matured MII oocytes.No significant difference was observed in embryo morphology from immature GV oocytes in IVF and IVM cycles.