CAJ | 학술논문

研究一种能够同时测定水产品中21种磺胺类药物残留量的检测方法--超高效液相色谱质谱法.样品经乙酸乙酯提取,经固相萃取小柱净化,氮气吹干后,用1mL流动相定容残余物.采用ACQUITY~(TM) BEH C_(18)色谱柱,用甲醇和5mmol/L乙酸铵溶液(含体积分数0.1%甲酸)作流动相,采用质谱技术分析.结果表明,检出限为0.01～0.20μg/kg,定量限为0.03～0.67μg/kg,平均回收率为68%～82%,相对标准偏差为4.1%～11.8%.该方法适合水产品中多种磺胺类药物的检测.
연구일충능구동시측정수산품중21충광알류약물잔류량적검측방법--초고효액상색보질보법.양품경을산을지제취,경고상췌취소주정화,담기취간후,용1mL류동상정용잔여물.채용ACQUITY~(TM) BEH C_(18)색보주,용갑순화5mmol/L을산안용액(함체적분수0.1%갑산)작류동상,채용질보기술분석.결과표명,검출한위0.01～0.20μg/kg,정량한위0.03～0.67μg/kg,평균회수솔위68%～82%,상대표준편차위4.1%～11.8%.해방법괄합수산품중다충광알류약물적검측.
An ultra performance liquid chromatography-electrospray ionization tandem mass spectrometric (UPLC-MS/MS) method has been developed for the simultaneous determination of 21 sulfonamide residues in aquatic products.Samples were extracted with ethyl acetate,cleaned up using an HLB SPE cartridge and blown to dryness under nitrogen,and the residues were then dissolved in 1 mL of mobile phase.The 21 analytes were separated on ACQUITY~(TM) BEH C_(18) column with a mobile phase composed of methanol and 5 mmol/L aqueous ammonium acetate solution (containing 0.1% formic acid).The limits for qualitative detection and quantitative determination were 0.01- 0.20μg/kg and 0.03-0.67μg/kg,respectively.The average recoveries at three spike levels ranged 68%-82%,with RSDs ranging 4.1%-11.8%.All these figures of merit demonstrate a good suitability of this method for the simultaneous determination of sulfonamide residues in aquatic products.