小粒野生稻酵母双杂交cDNA文库的构建
소립야생도효모쌍잡교cDNA문고적구건
Construction of Yeast Two-hybrid cDNA Library of Oryza minuta
  • 万方数据
    杂交水稻 잡교수도
    HYBRID RICE
    2010年 1期 67-69 ,共3页

    邢俊杰%陶小平%李玲龙%阳志刚%唐丽%李丁%谢灵灵%李莉%曹孟良

    형준걸%도소평%리령룡%양지강%당려%리정%사령령%리리%조맹량

    小粒野生稻%酵母双杂交%cDNA文库构建 小粒野生稻%酵母雙雜交%cDNA文庫構建
    소립야생도%효모쌍잡교%cDNA문고구건
    Oryza minuta%yeast two-hybrid system%construction of cDNA library
    采用酵母双杂交系统,利用SMART技术,在酵母菌株AH109中构建了小粒野生稻全长cDNA文库,文库容量约为1×10~6,插入片段平均大小约为640bp.该文库可以用于进一步的酵母双杂交筛选.
    채용효모쌍잡교계통,이용SMART기술,재효모균주AH109중구건료소립야생도전장cDNA문고,문고용량약위1×10~6,삽입편단평균대소약위640bp.해문고가이용우진일보적효모쌍잡교사선.
    With the yeast two-hybrid system,the whole-length cDNA library of Oryza minuta was constructed in the yeast strain AH109 by the SMART technique.There are about 1 × 10~6 clones in the library with an average of about 640bp in size of the insert fragments.The results showed that this library was good enough to be applied for the next step of selection in the yeast two-hybrid system.
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