CAJ | 학술논문

在实验室条件下,研究了高效降解菌2N3(克雷伯氏菌属,Klebsiella sp.)对被氯嘧磺隆污染土壤的修复作用及其影响因素.当土壤中氯嘧磺隆的添加浓度为20 mg/kg,每 1克土壤中2N3的接菌量为1×10~6个菌体时,第30 d时土壤中氯嘧磺隆的降解率为84.6%,对照仅为13.4%;相同2N3接菌浓度下,当土壤中氯嘧磺隆浓度为100 mg/kg时,其降解率为31.1%.以小麦、玉米、黄瓜为供试作物,在土壤中施加 20 mg/kg的氯嘧磺隆,当每 1克土壤中2N3的接菌量为1×10~6个菌体时,小麦、玉米、黄瓜的出苗率分别为85%,82%和79%,且处理组株高高于对照,表明降解菌2N3具有明显减轻氯嘧磺隆药害的作用.研究表明,人工接种降解菌2N3可提高土壤中氯嘧磺隆的降解率,有效降低其在土壤中的残留.
재실험실조건하,연구료고효강해균2N3(극뢰백씨균속,Klebsiella sp.)대피록밀광륭오염토양적수복작용급기영향인소.당토양중록밀광륭적첨가농도위20 mg/kg,매 1극토양중2N3적접균량위1×10~6개균체시,제30 d시토양중록밀광륭적강해솔위84.6%,대조부위13.4%;상동2N3접균농도하,당토양중록밀광륭농도위100 mg/kg시,기강해솔위31.1%.이소맥、옥미、황과위공시작물,재토양중시가 20 mg/kg적록밀광륭,당매 1극토양중2N3적접균량위1×10~6개균체시,소맥、옥미、황과적출묘솔분별위85%,82%화79%,차처리조주고고우대조,표명강해균2N3구유명현감경록밀광륭약해적작용.연구표명,인공접충강해균2N3가제고토양중록밀광륭적강해솔,유효강저기재토양중적잔류.
Bioremediation of Klebsiella sp. 2N3 strain in chlorimuron-ethyl contamined soil was examined under laboratory conditions. When 2N3 was inoculated into the soil at the density of 1×10~6 mycelium/g dry soil,chlorimuron-ethyl ( 20 mg/kg dry soil ) in soil was degraded by 84.6% after incubated for 30 days, with blank control of 13.4% degradation rate.When chlorimuron-ethyl was increased by 100 mg /kg in soil, chlorimuron-ethyl degradation rate was below 31.1%. With addition of chlorimuron-ethyl of 20 mg/kg in soil, and amount of strain 2N3 in 1×10~6 mycelium/g dry soil, emergence rates of wheat, corn and cucumber were 85%,82% and 79%,respectively. Plant heights were observed to increase in comparison with control plant.It's indicated that phyto-toxicity effect of chlorimuron-ethyl could be retarded by strain 2N3. The current study showed that the hand-actuated inoculation of 2N3's strain could effectively increase degradation rate of chlorimuron-ethyl in contaminated soil .