CAJ | 학술논문

利用发根农杆菌A_4菌株在西洋参根外植体上直接诱导产生发根,并分析不同浓度的植物激素6-BA、NAA,前体物醋酸镁(Mg(Ac)_2)、L-亮氨酸和诱导子茉莉酸甲酯(MJA)、水杨酸(SA)对西洋参(Panax quinquefo-lium L.)发根生长和皂苷含量的影响.同时,研究MJA与SA组合作用对皂苷含量的影响.采用高效液相色谱法测定发根中单体皂苷Rb_1的含量.结果显示,适宜浓度的外源物质均可促进皂苷含量的增加,MJA尤其明显.
이용발근농간균A_4균주재서양삼근외식체상직접유도산생발근,병분석불동농도적식물격소6-BA、NAA,전체물작산미(Mg(Ac)_2)、L-량안산화유도자말리산갑지(MJA)、수양산(SA)대서양삼(Panax quinquefo-lium L.)발근생장화조감함량적영향.동시,연구MJA여SA조합작용대조감함량적영향.채용고효액상색보법측정발근중단체조감Rb_1적함량.결과현시,괄의농도적외원물질균가촉진조감함량적증가,MJA우기명현.
Hairy roots of Panax quinquefolium L.were inducecl from the root explants of two-year-old Panax quinquefoli-um L.by Agrobacterium rhizogenes A_4 with directly inoculating.The growth speed and ginsenoside content of hairy root of Panax quinquefolium L.affected by phytohormones (6-BA, NAA), precursors (Mg (Ac)_2, L-leueine) and elicitors (MJA,SA) and the combination of MJA and SA were studied.The contents of ginsenoside Rb_1 have been determined with the method of high performance liquid chromatography.The feasible xenobiofics could promote the saponin accumu-lation,especially the MJA.