CAJ | 학술논문

旨在研究复合固态发酵(两株酵母和一株霉菌)对棉籽粕脱毒效果及营养价值的影响,同时分析测定发酵底物中的活性产物.结果表明:复合发酵可极显著降低棉籽粕底物游离棉酚含量(P<0.01),降解率达71.90%;棉籽粕底物粗蛋白、总氨基酸和必需氨基酸含量分别提高28.96%、8.83%、7.58%;发酵54～60 h时底物中蛋白酶活力最高(酸性蛋白酶活力为931.58 U/g,中性蛋白酶活力为1 097.79 U/g,碱性蛋白酶活力为798.62 U/g),纤维素酶活力最高(滤纸酶活力为1 601.66 U/g,Cx酶活力为3 590.02 U/g,C1酶活力为1 685.35 U/g),淀粉酶活力最高为578.05 U/g;同时发酵过程产生了一定量的有机酸.
지재연구복합고태발효(량주효모화일주매균)대면자박탈독효과급영양개치적영향,동시분석측정발효저물중적활성산물.결과표명:복합발효가겁현저강저면자박저물유리면분함량(P<0.01),강해솔체71.90%;면자박저물조단백、총안기산화필수안기산함량분별제고28.96%、8.83%、7.58%;발효54～60 h시저물중단백매활력최고(산성단백매활력위931.58 U/g,중성단백매활력위1 097.79 U/g,감성단백매활력위798.62 U/g),섬유소매활력최고(려지매활력위1 601.66 U/g,Cx매활력위3 590.02 U/g,C1매활력위1 685.35 U/g),정분매활력최고위578.05 U/g;동시발효과정산생료일정량적유궤산.
The effect of mixed culture solid fermentation on detoxification and nutritional value of cottonseed meal was investigated. Cottonseed meal was fermented with three strains ( Rhizopus oryzae, Candida oleophila and Kloeckera lindneri), then the reducing sugar content, protease activity, cellulase activity, amylase activity and organic acid content of the fermented cottenseed meal were analyzed, and its free gossypol (FG) content, crude protein (CP) content and amino acid contents were also determined. Results: The mixed culture fermentation significantly (P<0.01) decreases FG content in the fermented cottonseed meal, making a detoxification rate up to 71.90%. The CP content, total amino acids and essential amino acids are increased by 28.50%, 8.83%, 7.58%, respectively. The protease activities of the cottenseed meal after 54～60h fermentation are enhanced to peak values, namely, acid proteinase activity 931.58 U/g, neutral proteinase activity 1097.79 U/g, and alkaline protease activity 798.62 U/g; the cellulase activities are also enhanced to peak values, such as filter paper activity (FPA) 1601.66 U/g, Cx enzyme activity 3590.02 U/g, and C1 enzyme activity 1685.35 U/g. The amylase activity of the fermented eottenseed meal is enhanced to a peak value of 578.05 U/g. Meanwhile, a certain amount of organic acids are produced in the fermentation.