CAJ | 학술논문

目的:为深度开发米糠资源,研究纳豆芽孢杆菌发酵米糠提取物(rice bran formentation,RBF)的合生素的制备方法.方法:RBF经一定处理后进行真窄冷冻干燥,筛选出在冷冻干燥过程中效果较好的几种保护剂及其添加量,并模拟胃肠环境检测合生素制剂的耐受能力.结果:通过正交试验得出最佳的保护剂复合配方为脱脂奶粉添加量15%、蔗糖添加量5%、β-环状糊精添加量5%,该复合配方能显著提高纳豆菌的存活率,达到82.1%.模拟胃肠环境显示合生素制剂对胃酸、肠液、消化酶等的耐受能力较高,能在冷藏环境下储存较长时间.结论:RBF适于制备合生素制剂.
목적:위심도개발미강자원,연구납두아포간균발효미강제취물(rice bran formentation,RBF)적합생소적제비방법.방법:RBF경일정처리후진행진착냉동간조,사선출재냉동간조과정중효과교호적궤충보호제급기첨가량,병모의위장배경검측합생소제제적내수능력.결과:통과정교시험득출최가적보호제복합배방위탈지내분첨가량15%、자당첨가량5%、β-배상호정첨가량5%,해복합배방능현저제고납두균적존활솔,체도82.1%.모의위장배경현시합생소제제대위산、장액、소화매등적내수능력교고,능재랭장배경하저존교장시간.결론:RBF괄우제비합생소제제.
Rice bran was fermented with Bacillus natto and the 64-hour fermentation broth was separated by centrifugation to harvest supernatant.In the presence of protectant,the supernatant was vacuum lyophilized to produce a synbiotics.To maximize the survival rate of Bacillus natto,several protectants with good performance were chosen and formulated and subsequently,their optimal formulation was investigated using orthogonal arrays design.Moreover,the tolerance of synbiotics developed to simulated gastroenteric environment was explored.Results showed that the optimal compound lyophilization protectant formula consisted of skimmed milk powder 15%,sucrose 5% and β-cyclodextrin 5%.This formula resulted in an obvious increase of survival rate of Bacillus natto,reaching up to 82.1%.The synbiotics developed exhibited good tolerance to gastric fluid,intestinal fluid and digestive enzymes and could be stored for a long period of time under cold environment Its hydroxyl free radical scavenging rate presented a dramatic decrease during storage at 25 or 37℃,whereas only a slight decrease was observed at 4℃.