中药新药与临床药理
中藥新藥與臨床藥理
중약신약여림상약리
TRADITIONAL CHINESE DRUG RESEARCH&CLINICAL PHARMACOLOGY
2010年
2期
167-170
,共4页
柏冬%牛晓红%范斌%宋剑南
柏鼕%牛曉紅%範斌%宋劍南
백동%우효홍%범빈%송검남
肠外翻%桂枝汤%甘草苷%芍药苷%肉桂酸%甘草酸%肠吸收
腸外翻%桂枝湯%甘草苷%芍藥苷%肉桂痠%甘草痠%腸吸收
장외번%계지탕%감초감%작약감%육계산%감초산%장흡수
Everted gut%Guizhi decoction%Liquiritin%Paeoniflorin%Cinnamic acid%Glycyrrhizic acid%Intestinal absorption
目的 研究桂枝汤在肠道不同部位的吸收特征.方法 采用大鼠肠外翻模型,HPLC检测不同浓度桂枝汤中甘草苷、芍药苷、肉桂酸和甘草酸在肠道不同部位的吸收浓度,计算各成分的吸收参数,并分析它们在肠道不同部位的吸收特征.结果 不同浓度桂枝汤中甘草苷、芍药苷、肉桂酸和甘草酸在各肠道均为线性吸收,R~2均大于0.9,符合零级吸收速率;甘草苷在十二指肠、空肠、回肠,芍药苷、肉桂酸和甘草酸在十二指肠、空肠、回肠和结肠中的吸收速率常数(K_a)均随桂枝汤浓度的增加而增加(P<0.05),符合被动吸收;甘草苷在结肠中的吸收速率常数(K_a)随桂枝汤给药浓度的增加没有差异,符合主动转运;桂枝汤中甘草苷的最佳吸收部位为结肠,芍药苷和甘草酸的最佳吸收部位为十二指肠,肉桂酸在十二指肠和回肠中吸收均较好.结论 肠道不同部位对桂枝汤中不同成分的吸收具有一定差异,除甘草苷在结肠部位为主动吸收外,所有被测成分在不同肠道均为被动吸收形式.
目的 研究桂枝湯在腸道不同部位的吸收特徵.方法 採用大鼠腸外翻模型,HPLC檢測不同濃度桂枝湯中甘草苷、芍藥苷、肉桂痠和甘草痠在腸道不同部位的吸收濃度,計算各成分的吸收參數,併分析它們在腸道不同部位的吸收特徵.結果 不同濃度桂枝湯中甘草苷、芍藥苷、肉桂痠和甘草痠在各腸道均為線性吸收,R~2均大于0.9,符閤零級吸收速率;甘草苷在十二指腸、空腸、迴腸,芍藥苷、肉桂痠和甘草痠在十二指腸、空腸、迴腸和結腸中的吸收速率常數(K_a)均隨桂枝湯濃度的增加而增加(P<0.05),符閤被動吸收;甘草苷在結腸中的吸收速率常數(K_a)隨桂枝湯給藥濃度的增加沒有差異,符閤主動轉運;桂枝湯中甘草苷的最佳吸收部位為結腸,芍藥苷和甘草痠的最佳吸收部位為十二指腸,肉桂痠在十二指腸和迴腸中吸收均較好.結論 腸道不同部位對桂枝湯中不同成分的吸收具有一定差異,除甘草苷在結腸部位為主動吸收外,所有被測成分在不同腸道均為被動吸收形式.
목적 연구계지탕재장도불동부위적흡수특정.방법 채용대서장외번모형,HPLC검측불동농도계지탕중감초감、작약감、육계산화감초산재장도불동부위적흡수농도,계산각성분적흡수삼수,병분석타문재장도불동부위적흡수특정.결과 불동농도계지탕중감초감、작약감、육계산화감초산재각장도균위선성흡수,R~2균대우0.9,부합령급흡수속솔;감초감재십이지장、공장、회장,작약감、육계산화감초산재십이지장、공장、회장화결장중적흡수속솔상수(K_a)균수계지탕농도적증가이증가(P<0.05),부합피동흡수;감초감재결장중적흡수속솔상수(K_a)수계지탕급약농도적증가몰유차이,부합주동전운;계지탕중감초감적최가흡수부위위결장,작약감화감초산적최가흡수부위위십이지장,육계산재십이지장화회장중흡수균교호.결론 장도불동부위대계지탕중불동성분적흡수구유일정차이,제감초감재결장부위위주동흡수외,소유피측성분재불동장도균위피동흡수형식.
Objective To study the absorption of Guizhi decoction at different intestinal section. Methods In-vitro everted gut model was applied. The absorption concentration of liquiritin, paeoniflorin, cinnamic acid and glycyrrhizic acid, which are representative compositions of the Guizhi decoction, were detected by HPLC, and the absorption parameter was calculated for describing the absorption character. Results The absorption curve of liquiritin, paeoniflorin, cinnamic acid and glycyrrhizic acid from different dosages of Guizhi decoction were linear. The K_a of liquiritin in the duodenum, jejunum and ileum, as well as paeoniflorin, cinnamic acid and glycyrrhizic acid in the duodenum, jejunum ileum and colon, increased along with the raised dosage of the Guizhi decoction (P < 0.05) , indicating a passive absorption manner. The K_a of liquiritin in the colon was invariable along with the raised dosage, indicating an active transport. The absorption of liquiritin in the colon was better than other intestines, while paeoniflorin and glycyrrhizic acid can be absorbed better in duodenum, cinnamic acid can be absorbed better in both the duodenum and ileum. Conclusion Except that liquiritin is in active absorption in the colon, the absorption of liquiritin, paeoniflorin, cinnamic acid and glycyrrhizic acid from different dosages of Guizhi decoction is in passive absorption.
