CAJ | 학술논문

目的观察单个核细胞趋化蛋白-1MCP-1及其受体CCR2在多发性骨髓瘤(MM)患者骨髓瘤细胞和骨髓基质细胞中的表达.方法经国内统一标准确诊为MM的患者15例,男7例,女8例,年龄38～67岁,平均年龄53.7岁.按照Durie-Salmon分期系统分期,Ⅰ期2例、Ⅱ期5例、Ⅲ期8例.其他血液病患者10例为对照组.取样本进行骨髓瘤细胞与骨髓基质细胞的分离培养,在含13％胎牛血清的RPMI 1640培养液中培养,每2d更换1次培养液,实验用细胞均处于对数生长期.采用流式细胞术检测骨髓瘤患者骨髓瘤细胞,骨髓基质细胞中MCP-1、CCR2水平.结果 14例MM患者骨髓均表达MCP-1、CCR2,而对照组8例骨髓中趋化因子MCP-1、CCR2基本无表达.对照组和MM患者基质细胞上MCP-1、CCR2均阳性的表达率分别为4.27％和68.71％,两组比较差异具有统计学意义(P＜0.05).瘤细胞上MCP-1、CCR2均阳性的表达率分别为3.25％和32.76％,两组比较差异具有统计学意义(P＜0.05).处于疾病活动期的基质细胞和瘤细胞上MCP-1、CCR2均阳性的表达率分别为68.71％和32.76％,而处于疾病非活动期的的基质细胞和瘤细胞上MCP-1、CCR2均阳性的表达率分别为70.12％和53.39％,两组比较差异无统计学意义(P＞0.05).结论大部分MM患者骨髓均表达MCP-1、CCR2.MCP-1及CCR2是MM细胞表面表达的主要趋化因子,对MM的疾病进展起着一个比较重要的作用.
목적 관찰단개핵세포추화단백-1MCP-1급기수체CCR2재다발성골수류(MM)환자골수류세포화골수기질세포중적표체.방법 경국내통일표준학진위MM적환자15례,남7례,녀8례,년령38～67세,평균년령53.7세.안조Durie-Salmon분기계통분기,Ⅰ기2례、Ⅱ기5례、Ⅲ기8례.기타혈액병환자10례위대조조.취양본진행골수류세포여골수기질세포적분리배양,재함13％태우혈청적RPMI 1640배양액중배양,매2d경환1차배양액,실험용세포균처우대수생장기.채용류식세포술검측골수류환자골수류세포,골수기질세포중MCP-1、CCR2수평.결과 14례MM환자골수균표체MCP-1、CCR2,이대조조8례골수중추화인자MCP-1、CCR2기본무표체.대조조화MM환자기질세포상MCP-1、CCR2균양성적표체솔분별위4.27％화68.71％,량조비교차이구유통계학의의(P＜0.05).류세포상MCP-1、CCR2균양성적표체솔분별위3.25％화32.76％,량조비교차이구유통계학의의(P＜0.05).처우질병활동기적기질세포화류세포상MCP-1、CCR2균양성적표체솔분별위68.71％화32.76％,이처우질병비활동기적적기질세포화류세포상MCP-1、CCR2균양성적표체솔분별위70.12％화53.39％,량조비교차이무통계학의의(P＞0.05).결론 대부분MM환자골수균표체MCP-1、CCR2.MCP-1급CCR2시MM세포표면표체적주요추화인자,대MM적질병진전기착일개비교중요적작용.
Objective To observe the expression of chemokine (MCP-1) and chemokine receptor (CCR2) in bone marrow cells,bone marrow stromal cells of multiple myeloma (MM) patients.Methods 15 cases were diagnosed by domestic uniform standard for MM patients,7 cases of male,8 cases of female,age range from 38 to 67 years,mean age 53.7 years old.According to the Durie-Salmon staging system,patients were divided into Ⅰ (2 cases),Ⅱ (5 cases) and Ⅲ period(8 cases).Control group were from 10 cases of non-malignant blood disease patients.MCP-1,CCR2 expression were measured by flow cytometry.Results Almost 14 cases of bone marrow cells expressed MCP-1and CCR2 in MM patients,while in the control group,bone marrow cells almost did not express MCP-1and CCR2.Stromal cells had similar MCP-1and CCR2 expression profile (68.17 ％ vs 4.27 ％. P＜0.05).Tumor cells of MCP-1/CCR2 expression rates were 3.25 ％ and 32.76 ％. Compared MCP-1/ CCR2 expression of stromal cells and tumor cells with different stages of disease, the activated stage and the stable stage had similar level (68.71％ and 32.76 ％ vs 70.12 ％ and 53.39 ％. P＞0.05). Conclusion Most patients with MM bone marrow were expressed MCP-1and CCR2.MCP-1and CCR2 are the major MM cell surface expression of chemokine/receptor, which play important roles in the progress of.