矽尘对大鼠肺表面活性蛋白影响的研究
석진대대서폐표면활성단백영향적연구
The change of pulmonary surfactant protein of rat following silica exposure
  • 万方数据
    中华劳动卫生职业病杂志 중화노동위생직업병잡지
    CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
    2012年 9期 667-671 ,共5页

    翟瑞雪%姚林%姚雪%闫立成%郝玉兰%关维俊%张俊%张艳淑

    적서설%요림%요설%염립성%학옥란%관유준%장준%장염숙

    矽肺%肺表面活性蛋白%羟脯氨酸 矽肺%肺錶麵活性蛋白%羥脯氨痠
    석폐%폐표면활성단백%간포안산
    Silicosis%Lung surfactant protein%Hydroxyproline
    目的 探讨不同矽尘暴露时间对于大鼠肺表面活性蛋白(SP)A、B、C、D的影响,为矽肺的早期诊断和临床治疗提供理论依据.方法 将60只大鼠随机分为染尘组和相应对照组,每组30只.染尘组大鼠经气管灌注50 mg/ml的粉尘1 ml,左右两肺各0.5 ml；对照组灌注等量的生理盐水.在染尘后第3、7、14、21、28天分别获取血清和支气管肺泡灌洗液(BALF),应用酶联免疫法(ELISA)检测大鼠血 清和BALF中SP-A、SP-B、SP-C、SP-D的含量.同时检测肺组织中总抗氧化能力(T-AOC)和羟脯氨酸(HYP)的含量,对肺组织进行HE染色观察病理变化.结果 与对照组比较,3、14、21、28 d染尘组大鼠BALF中SP-A含量及各时点染尘组大鼠BALF中SP-D含量均明显降低,差异均有统计学意义(P＜0.05)；与对照组比较,7、14、21、28 d染尘组大鼠BALF中SP-B含量及14、21、28 d染尘组大鼠BALF中SP-C含量明显升高,差异有统计学意义(P＜0.05).与对照组比较,14、21、28 d染尘组大鼠血清中SP-A含量及7、14、21d染尘组大鼠血清中SP-B含量及7、14、21、28 d染尘组大鼠血清中SP-C含量明显增加,差异均有统计学意义(P＜0.05),且随染矽尘时间的延长,血清中SP-C含量呈增加趋势,有时间-效应关系(r=0.618,P=0.042).与对照组比较,7、14、21、28 d染尘组大鼠血清中SP-D含量降低,差异有统计学意义(P＜0.05)；随着染尘时间的增加,血清中SP-D含量呈下降的趋势,有时间-效应关系(r=-0.731,P=0.016).与对照组比较,3、7、14、21、28 d染尘组大鼠肺组织中HYP含量升高,7、14、21、28 d染尘组大鼠肺组织中T-AOC含量下降,差异均有统计学意义(P＜0.05).染尘大鼠BALF中SP-C的含量与肺组织中HYP呈正相关(r=0.803,P=0.045)；BALL中SP-D的含量与肺组织中HYP呈负相关(r=-0.867,P=0.033).BALF中SP-A、SP-B含量与肺组织中HYP无相关(r=0.416,P=0.28；r=0.592,P=0.071).BALF与血清中SP-D含量的均呈下降趋势,呈正相关(r=0.870,P=0.034).BALF与血清中SP-C含量均增加呈正相关(r=0.539,P=0.046).染尘组大鼠肺组织病理观察可见,肺泡间隔逐渐增厚,尘细胞浸润,有少量胶原纤维分布,至28 d时,出现尘细胞结节.结论 矽尘导致大鼠BALF中SP含量改变,血清中SP-C、SP-D的水平可作为矽肺早期效应标志物.
    목적 탐토불동석진폭로시간대우대서폐표면활성단백(SP)A、B、C、D적영향,위석폐적조기진단화림상치료제공이론의거.방법 장60지대서수궤분위염진조화상응대조조,매조30지.염진조대서경기관관주50 mg/ml적분진1 ml,좌우량폐각0.5 ml；대조조관주등량적생리염수.재염진후제3、7、14、21、28천분별획취혈청화지기관폐포관세액(BALF),응용매련면역법(ELISA)검측대서혈 청화BALF중SP-A、SP-B、SP-C、SP-D적함량.동시검측폐조직중총항양화능력(T-AOC)화간포안산(HYP)적함량,대폐조직진행HE염색관찰병리변화.결과 여대조조비교,3、14、21、28 d염진조대서BALF중SP-A함량급각시점염진조대서BALF중SP-D함량균명현강저,차이균유통계학의의(P＜0.05)；여대조조비교,7、14、21、28 d염진조대서BALF중SP-B함량급14、21、28 d염진조대서BALF중SP-C함량명현승고,차이유통계학의의(P＜0.05).여대조조비교,14、21、28 d염진조대서혈청중SP-A함량급7、14、21d염진조대서혈청중SP-B함량급7、14、21、28 d염진조대서혈청중SP-C함량명현증가,차이균유통계학의의(P＜0.05),차수염석진시간적연장,혈청중SP-C함량정증가추세,유시간-효응관계(r=0.618,P=0.042).여대조조비교,7、14、21、28 d염진조대서혈청중SP-D함량강저,차이유통계학의의(P＜0.05)；수착염진시간적증가,혈청중SP-D함량정하강적추세,유시간-효응관계(r=-0.731,P=0.016).여대조조비교,3、7、14、21、28 d염진조대서폐조직중HYP함량승고,7、14、21、28 d염진조대서폐조직중T-AOC함량하강,차이균유통계학의의(P＜0.05).염진대서BALF중SP-C적함량여폐조직중HYP정정상관(r=0.803,P=0.045)；BALL중SP-D적함량여폐조직중HYP정부상관(r=-0.867,P=0.033).BALF중SP-A、SP-B함량여폐조직중HYP무상관(r=0.416,P=0.28；r=0.592,P=0.071).BALF여혈청중SP-D함량적균정하강추세,정정상관(r=0.870,P=0.034).BALF여혈청중SP-C함량균증가정정상관(r=0.539,P=0.046).염진조대서폐조직병리관찰가견,폐포간격축점증후,진세포침윤,유소량효원섬유분포,지28 d시,출현진세포결절.결론 석진도치대서BALF중SP함량개변,혈청중SP-C、SP-D적수평가작위석폐조기효응표지물.
    Objective To investigate the change of lung surfactant protein (SP) A,B,C,D of rats following silica dust exposure in order to provide the evidences for the early diagnosis indices or therapy of silicosis.Methods 60 male SD rats were randomly divided into silica group,and corresponding controls group.Rats in silica group were administrated 1 ml silica solution by intratracheal instillation at dose of 50mg/ml.Rats in control group were administrated the same amount saline.At 3rd,7th,14th,21st,28th after silica exposure,serum and bronchoalveolar lavage fluid (BALF) samples were obtained.The concentration of SP-A,SP-B,SP-C,SP-D in serum and BALF were measured by using enzyme immunoassay (ELISA).Meanwhile the levels of total anti-oxidative activity (T-AOC) and hydroxyproline (HYP) in lung tissue were also detected.The pathology of lung tissue was conducted.Results Compared with control group,SP-A concentration in BALF of silica exposed rat for 3,14,21,28 d was significant lower and SP-D concentration in BALF of silica exposed rat for all time points was also lower.The differences were significant (P＜0.05).Meanwhile SP-B level in 7、14、21、28d silica exposed rats BALF and SP-C level in 14、21、28d silica exposed rats markedly decreased (P＜0.05).In addition compared with control group,SP-A,SP-B and SP-C concentration in serum of silica exposed rat were higher when SP-A for 14,21,28 d silica exposure,SP-B for 7,14,21 d silica exposure and Sp-C for 7,14,21,28 d exposure.And all difference were significant (P＜0.05).As silica exposure time increased,SP-C concentration in serum showed an increase trend,which showed a time-response relationship (r=0.618,P=0.042).However,SP-D concentration in serum of rat for 7,14,21,28 d silica exposure were significant lower than that of control group (P＜0.05).And there was a decrease trend with time point exposure regarding of SP-D (r=-0.731,P=0.016).The HYP content in lung tissue of experiment rats increased at 3rd,7th,14th,21st and 28th day time point and The T-AOC activity in lung tissue decrease at,7th,14th,21st and 28th day time point.The differences were significant (P＜0.05).There was a positive correlation (P=0.803,P=0.045) between SP-C in BALF and HYP of silica exposed rats and a negative correlation between SP-D in BALF and HYP (r=-0.867,P=0.033).No significant correlation were seen between SP-A,SP-B BALF and HYP(y=0.416,P=0.28; r=0.592,P=0.071).SP-C concentration in BALF and serum all showed an increased trend and a positive correlation was seen (r=0.539,P=0.046).The same decrease trend was seen between SP-D in BALF and serum and correlation value was 0.870 (P=0.034).Conclusion The silica exposure did cause the change of SP content both in BALF and serum.The SP-C and SP-D content in serum might be served as an early effective biomarker of silicosis.
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