中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2011年
3期
232-236
,共5页
刘大男%何作云%方颖%吴立荣%刘兴德%李屏%严征
劉大男%何作雲%方穎%吳立榮%劉興德%李屏%嚴徵
류대남%하작운%방영%오립영%류흥덕%리병%엄정
血红素加氧酶-1%颈动脉狭窄%一氧化碳
血紅素加氧酶-1%頸動脈狹窄%一氧化碳
혈홍소가양매-1%경동맥협착%일양화탄
Heme oxygenase-1%Carotid stenosis%Carbon monoxide
目的 研究血红素氧合酶-1(HO-1)与一氧化碳(CO)系统对兔颈动脉球囊损伤后再狭窄血管的保护作用及其可能的机制.方法 新西兰大白兔随机分为5组:对照组、假手术组、胆固醇组、血红素组和卟啉锌组.对照组予普通饲料,其余4组喂饲含1.5%胆固醇饲料,血红素组和卟啉锌组同时分别予氯化血红素或锌原卟啉-9腹腔内注射,2周后行颈总动脉球囊损伤,术后继续原方法喂养给药8周.结果 与对照组比较,胆固醇组颈动脉一氧化氮合酶活性、一氧化氮生成量显著降低,而HO-1表达、CO生成量显著增加(均P＜0.01);与胆固醇组比较,血红素组HO-1表达、CO生成量增加,同时内皮素-1表达降低,内膜厚度和内膜增殖指数最小[分别为(442.17±59.14)μm和3.99±0.52与(281.47±21.10)μm和2.49±0.17,均P＜0.01];卟啉锌组HO-1表达、CO生成量降低,而内皮素-1表达增高,内膜厚度和内膜增殖指数最大[(698.71±58.37)um和6.17±0.52,均P＜0.01].结论 HO-1与CO系统可能通过代偿和调节一氧化氮合酶和(或)一氧化氮系统以及降低内皮素-1表达,对血管损伤后内皮功能、内膜增生和血管重塑起到保护作用,从而抑制再狭窄.
目的 研究血紅素氧閤酶-1(HO-1)與一氧化碳(CO)繫統對兔頸動脈毬囊損傷後再狹窄血管的保護作用及其可能的機製.方法 新西蘭大白兔隨機分為5組:對照組、假手術組、膽固醇組、血紅素組和卟啉鋅組.對照組予普通飼料,其餘4組餵飼含1.5%膽固醇飼料,血紅素組和卟啉鋅組同時分彆予氯化血紅素或鋅原卟啉-9腹腔內註射,2週後行頸總動脈毬囊損傷,術後繼續原方法餵養給藥8週.結果 與對照組比較,膽固醇組頸動脈一氧化氮閤酶活性、一氧化氮生成量顯著降低,而HO-1錶達、CO生成量顯著增加(均P＜0.01);與膽固醇組比較,血紅素組HO-1錶達、CO生成量增加,同時內皮素-1錶達降低,內膜厚度和內膜增殖指數最小[分彆為(442.17±59.14)μm和3.99±0.52與(281.47±21.10)μm和2.49±0.17,均P＜0.01];卟啉鋅組HO-1錶達、CO生成量降低,而內皮素-1錶達增高,內膜厚度和內膜增殖指數最大[(698.71±58.37)um和6.17±0.52,均P＜0.01].結論 HO-1與CO繫統可能通過代償和調節一氧化氮閤酶和(或)一氧化氮繫統以及降低內皮素-1錶達,對血管損傷後內皮功能、內膜增生和血管重塑起到保護作用,從而抑製再狹窄.
목적 연구혈홍소양합매-1(HO-1)여일양화탄(CO)계통대토경동맥구낭손상후재협착혈관적보호작용급기가능적궤제.방법 신서란대백토수궤분위5조:대조조、가수술조、담고순조、혈홍소조화계람자조.대조조여보통사료,기여4조위사함1.5%담고순사료,혈홍소조화계람자조동시분별여록화혈홍소혹자원계람-9복강내주사,2주후행경총동맥구낭손상,술후계속원방법위양급약8주.결과 여대조조비교,담고순조경동맥일양화담합매활성、일양화담생성량현저강저,이HO-1표체、CO생성량현저증가(균P＜0.01);여담고순조비교,혈홍소조HO-1표체、CO생성량증가,동시내피소-1표체강저,내막후도화내막증식지수최소[분별위(442.17±59.14)μm화3.99±0.52여(281.47±21.10)μm화2.49±0.17,균P＜0.01];계람자조HO-1표체、CO생성량강저,이내피소-1표체증고,내막후도화내막증식지수최대[(698.71±58.37)um화6.17±0.52,균P＜0.01].결론 HO-1여CO계통가능통과대상화조절일양화담합매화(혹)일양화담계통이급강저내피소-1표체,대혈관손상후내피공능、내막증생화혈관중소기도보호작용,종이억제재협착.
Objective To investigate the protective effect of heme oxygenase-1 (HO-1)/carbon monoxide (CO) system on restenosis after balloon angioplasty and relative mechanism. Methods Fifty rabbits were randomly divided into 5 groups. Control group received normal chow (Control group), the other rabbits received 1.5% cholesterol diet (Chol group and SH group) or 1.5%cholesterol diet plus hemin (Hem group) or zinc protoporphyrin Ⅸ (Znpp group) for 10 weeks. At the third week of experiment, the three experimental groups underwent balloon injury at one side common carotid artery. Results Compared with control group, arterial nitric oxide production and nitricoxide synthase activity were significantly decreased, while HO-1 expression and CO production were significantly increased (all P＜0. 01 ) in Chol group. The intima thickness and ratio of intima/media (I/M) were lower in Hem group than in Chol group [(281.47± 21.10) μm vs. (442.17 ±59.14) μm, 2.49 ± 0. 17 vs. 3.99 ± 0. 52, respectively]. While arterial HO-1 expression and CO production were increased markedly, endothelin-1 expression was distinctly reduced in Hem group group than in Chol group. Compared with Chol group, arterial HO-1 expression and CO production were decreased obviously, while endothelin-1 expression and intima thickness and ratio of intima/media [(698.71±58. 37) μm, 6.17±0. 52]were significantly increased in Znpp group (all P＜0. 01).Conclusions The HO-1/CO system plays a protective role on improving endothelium function and restraining neointimal proliferation by compensating and regulating nitricoxide synthase/nitric oxide system and lowering endothelin -1 expression so as to inhibit restenosis after balloon injury.
