CAJ | 학술논문

目的对比加叠氮钠和传统的去氧胆酸钠法去除细胞后心脏瓣膜形态学及生物力学差别,为构建理想的组织工程心脏瓣膜提供实验依据.方法应用两种方法处理6～7月龄新鲜猪主动脉心脏瓣膜.光学显微镜、透射电镜观察脱细胞基质改变,厚度仪测量组织厚度,行拉力测试观察两种方法处理后力学变化上的差异.结果两种方法处理后组织厚度差异无统计学意义(P>0.01).显微镜检和透射电镜见叠氮钠-去氧胆酸钠法对基质破坏较少,处理后生物力学优于传统去氧胆酸钠法,差异有统计学意义(P<0.01).结论叠氮钠-去氧胆酸钠法能更好保护去除瓣叶组织后的细胞外基质.
목적 대비가첩담납화전통적거양담산납법거제세포후심장판막형태학급생물역학차별,위구건이상적조직공정심장판막제공실험의거.방법 응용량충방법처리6～7월령신선저주동맥심장판막.광학현미경、투사전경관찰탈세포기질개변,후도의측량조직후도,행랍력측시관찰량충방법처리후역학변화상적차이.결과 량충방법처리후조직후도차이무통계학의의(P>0.01).현미경검화투사전경견첩담납-거양담산납법대기질파배교소,처리후생물역학우우전통거양담산납법,차이유통계학의의(P<0.01).결론 첩담납-거양담산납법능경호보호거제판협조직후적세포외기질.
Objective Heart valves made by tissue engineering procedure may be superior to those by converaitional procedure. Optimal techniques for decellularization farm pretreated heart valves should preserve maximum matrix, which is associated directly withthe mechanical strength and durability of the valves. The study was designed to compare the morphology and biomechanics of the matrix after decellularization procedures with conventional aodium-deoxycholate method and the addition d sodium azide to the fonner method, in order to provide experimental basis for producing ideal tissue-engineered heart valves. Methods Fresh aortic valves from porcine of 6 to 7 month were divided randomly into two groups (60 valve leaflets in each group). Valves in one group were treated with Triton-X100 and sodium deoxycholate, and those in the other group were treated with identical chemicals and sodium azide. Changes of the matrix structure and effects of treatment on the collagen and elastic fibers were observed by microscopy(with hematoxylin and eosin stain, Masson's trichrome stain and Weigert's stain for elastic fiber) and transmission electron microscope. Tissue thickness (60 specimens) and biomechanics properties (50 specimens) were measured. Biomechanics characteristics measured after decellularization weer maximum deflection, elongation rate, max tensile stress and max load. Statistical analysis was performed to compare the thickness and mechanical properties after two decellularization procedures. Results No significant difference in tissue thiekness was found be-tween two methods(P > 0.01), but biomechanics properties-maximum deflection, elongation rate, max tensile stress and max load-were increased significantly in the group with sodium azide (P <0.01). Same results could be obtained through Tensile deflection-Tensile stress and Tensile deflection-Load curve. Although complete decellularization was achieved, matrix structure was comparative integrity in the group treated with sodium azide. Intact, dense collagen fibers and plush-like fibers were seen in the experimental Stoup, while sparse collagen fibers and less velvet-like fibers were present in the control group. Complete and continuous elastic fibers were preserved in the specimens treated with sodium azide while discontinuous, broken and thin fibers were seen in the control group. The pattern d ultrastructure in the sodimn azide group revealed matrix in high density and more fiber bundles in the field. In the com-trol group, the quality of the matrix decreased significantly, and loose fibers with apparent gap were seen. Conclusion Sodium azide can preserve the matrix structure efficiently during the decellurazation procedure and improve the bio-mechanical properties of tissue engineered cardiac valves.