CAJ | 학술논문

目的深入研究维甲酸诱导基因G(retinoic acid-induced gene G,RIG-G)启动子上所含的干扰素刺激反应元件(interferon-stimulated response elements,ISRE)对RIG-G基因表达的调控作用.方法根据RIG-G基因启动子所包含的ISRE序列,利用定点突变技术分别构建野生型和位点突变型的报告基因质粒,然后采用报告基因转染实验检测RIG-G基因启动子中ISRE序列的功能活性.结果研究发现单独突变RIG-G基因启动子上的ISRE Ⅱ元件不影响报告基因的表达,而单独突变ISRE Ⅰ则会对报告基因的表达产生明显的抑制作用;同时突变ISRE Ⅰ和ISRE Ⅱ元件则会使报告基因完全失去对转录因子的反应性.结论 RIG-G基因启动子所包含的ISRE Ⅰ和ISRE Ⅱ元件是诱导该基因表达的转录因子复合物的作用位点,是该基因表达的分子基础,且ISRE Ⅰ元件的作用要优先于ISRE Ⅱ.
목적 심입연구유갑산유도기인G(retinoic acid-induced gene G,RIG-G)계동자상소함적간우소자격반응원건(interferon-stimulated response elements,ISRE)대RIG-G기인표체적조공작용.방법 근거RIG-G기인계동자소포함적ISRE서렬,이용정점돌변기술분별구건야생형화위점돌변형적보고기인질립,연후채용보고기인전염실험검측RIG-G기인계동자중ISRE서렬적공능활성.결과 연구발현단독돌변RIG-G기인계동자상적ISRE Ⅱ원건불영향보고기인적표체,이단독돌변ISRE Ⅰ칙회대보고기인적표체산생명현적억제작용;동시돌변ISRE Ⅰ화ISRE Ⅱ원건칙회사보고기인완전실거대전록인자적반응성.결론 RIG-G기인계동자소포함적ISRE Ⅰ화ISRE Ⅱ원건시유도해기인표체적전록인자복합물적작용위점,시해기인표체적분자기출,차ISRE Ⅰ원건적작용요우선우ISRE Ⅱ.
Objective To study the regulatory role of interferon-stimulated response elements (ISREs) located on the retinoic acid-induced gene G (RIG-G) promoter in RIG-G expression. Methods By using point mutation technique, the authors constructed the wide type and site-mutant reporter gene plasmids according to the ISRE sequence on RIG-G promoter, and detected the functional activities by luciferase reporter assay. Results Mutation in ISRE Ⅱ alone had no obvious effect on the expression of the reporter gene, whereas mutation in ISRE Ⅰ dramatically inhibited the transactivity of RIG-G promoter. Mutation in both ISRE Ⅰ and ISRE Ⅱ resulted in complete loss of its response to the transcription factors for the reporter gene. Conclusion Both ISRE Ⅰ and ISRE Ⅱ on the RIG-G promoter are the binding sites for the complex of transcription factors. They are required for RIG-G expression, and ISRE Ⅰ has a preferential role over ISRE Ⅱ .