CAJ | 학술논문

目的观察组织蛋白酶B(CatB)在颅内动瘤内的表达情况,评估其对瘤壁血管平滑肌细胞凋亡的作用,以此揭示颅内动脉瘤可能形成的机制.方法以2006年11月至2009年2月的20例显微手术切除的颅内动脉瘤标本为研究对象,取脑外伤后内减压术切除的正常脑皮层动脉血管6例作为对照,通过透射电镜了解其超微结构变化;应用免疫组化的方法检测标本中CatB、α平滑肌激动蛋白(α-SMA)和半胱大冬酶-3(Caspase-3)的表达,并对染色强度进行分级;通过Real-time PCR的方法检测标本内的CatB mRNA表达.结果 (1)免疫组化结果:动脉瘤组CatB、Caspase-3表达指数高于对照组.而动脉瘤组α-SMA表达低于对照组(P<0.05);CatB与caspase-3、α-SMA及动脉瘤的大小具有相关性(P<0.01).(2)Real-time PCR:CatB mRNA在颅内动脉瘤的表达为对照组的3.8倍(P<0.01).(3)透射电镜的观察:动脉瘤瘤壁血管平滑肌细胞明显减少,基质纤维断裂消失;部分血管平滑肌细胞核内见染色体边聚,核固缩,并有胞浆或胞核部分脱落或裂解成碎片,还有部分瘤壁可见典型的凋亡小体.结论 CatB可能参与了颅内动脉瘤的形成与生长.
목적 관찰조직단백매B(CatB)재로내동류내적표체정황,평고기대류벽혈관평활기세포조망적작용,이차게시로내동맥류가능형성적궤제.방법 이2006년11월지2009년2월적20례현미수술절제적로내동맥류표본위연구대상,취뇌외상후내감압술절제적정상뇌피층동맥혈관6례작위대조,통과투사전경료해기초미결구변화;응용면역조화적방법검측표본중CatB、α평활기격동단백(α-SMA)화반광대동매-3(Caspase-3)적표체,병대염색강도진행분급;통과Real-time PCR적방법검측표본내적CatB mRNA표체.결과 (1)면역조화결과:동맥류조CatB、Caspase-3표체지수고우대조조.이동맥류조α-SMA표체저우대조조(P<0.05);CatB여caspase-3、α-SMA급동맥류적대소구유상관성(P<0.01).(2)Real-time PCR:CatB mRNA재로내동맥류적표체위대조조적3.8배(P<0.01).(3)투사전경적관찰:동맥류류벽혈관평활기세포명현감소,기질섬유단렬소실;부분혈관평활기세포핵내견염색체변취,핵고축,병유포장혹포핵부분탈락혹렬해성쇄편,환유부분류벽가견전형적조망소체.결론 CatB가능삼여료로내동맥류적형성여생장.
Objectives To detect the expression of Cathepin B(CatB) in the intracranial aneurysm wall and its effect to the apoptosis of vascular smooth muscle cells, aimed at clarifying the pathological formation mechanism of intracranial aneurysm. Methods From November 2006 to Februry 2009, 20 intracranial aneurysm samples were collected as the experimental group, and 6 cases of normal pallium artery samples were collected as the control group. Immunohistochemical technique was used to evaluate the expressions of CatB, α-smooth muscle actin ( α-SMA ) and Caspase-3. The expression of CatB mRNA was evaluated by real-time PCR. The ultrastructure of intracranial aneurysms were observed by using the transmission electronic microscope. Results Compared with the normal pallium artery specimens, the expression of CatB and Caspase-3 both significantly increased in the intracranial aneurysm walls where α-SMA decreased(P<0. 05). The mean expression of CatB mRNA in intracranial aneurysm samples was about 3. 8-folds than that in control group ( P < 0. 01). There were excessive apoptotic vascular smooth muscle cells (VSMCs) in the tunica median,and typical apoptotic body were observed in some aneurysm walls. Conclusion Cathepin B may be involved in the formation and the progression of intracranial aneurysm.