CAJ | 학술논문

从菠菜（Spinacia oleracea L.）叶绿体中提取的PSⅠ 颗粒中加入不同浓度的组氨酸，利用光谱和SDS_PAGE技术研究了强光（2 300 μmol*m-2*s-1）处理过程中外加组氨酸对色素和多肽光破坏进程的影响。强光处理可以使PSⅠ颗粒的光吸收减小，在照光30 min后外加组氨酸有效地抑制了光吸收减小的趋势。外加组氨酸在照光约10 min后对PSⅠ颗粒CD信号的下降也起到了明显的抑制作用。外加组氨酸对PSⅠ颗粒中色素保护作用的这种延迟现象表明，在强光照初期和后期PSⅠ颗粒光抑制的机理可能不同。外加组氨酸还可以有效地抑制光照过程中PSⅠ颗粒77 K荧光产量的下降。SDS_PAGE分析结果发现，外加组氨酸在光照过程中不但对PSⅠ颗粒的反应中心蛋白可以起到保护作用，对其他多肽组分同样有显著的保护作用。
종파채（Spinacia oleracea L.）협록체중제취적PSⅠ 과립중가입불동농도적조안산，이용광보화SDS_PAGE기술연구료강광（2 300 μmol*m-2*s-1）처리과정중외가조안산대색소화다태광파배진정적영향。강광처리가이사PSⅠ과립적광흡수감소，재조광30 min후외가조안산유효지억제료광흡수감소적추세。외가조안산재조광약10 min후대PSⅠ과립CD신호적하강야기도료명현적억제작용。외가조안산대PSⅠ과립중색소보호작용적저충연지현상표명，재강광조초기화후기PSⅠ과립광억제적궤리가능불동。외가조안산환가이유효지억제광조과정중PSⅠ과립77 K형광산량적하강。SDS_PAGE분석결과발현，외가조안산재광조과정중불단대PSⅠ과립적반응중심단백가이기도보호작용，대기타다태조분동양유현저적보호작용。
The protecting effect of histidine on the photodamage of pigments and proteins of the isolated PSⅠ particles from the chloroplast of Spinacia oleracea L. during the strong illumination (2 300 μmol*m-2*s-1) was studied by spectroscopy and SDS_PAGE. The absorbance of PSⅠ particles decreased during the strong illumination treatment, but the decrease would be slowed down in the presence of externally added histidine after 30 min illumination. The decrease of CD （circular dichroism）　signal intensities of PSⅠ particles also was slowed down by the added histidine after about 10 min illumination. The retarded protecting effect of the added histidine on the photobleaching of pigments of PSⅠ complexes implied that the mechanisms of photoinhibition of isolated PSⅠ complexes are different from early stage to later stage during the strong illumination treatment. In addition, the added histidine suppressed the decrease of 77 K fluorescence yield of PSⅠ particles during the illumination. SDS_PAGE showed that the added histidine not only protected the reaction center proteins of PSⅠ particles, but also protected other subunits of PSⅠ particles from degradation.