南京林业大学学报（自然科学版）
南京林業大學學報（自然科學版）
남경임업대학학보（자연과학판）
JOURNAL OF NANJING FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2005年
1期
20-24
,共5页
阮成江%钦佩%韩睿明%何祯祥
阮成江%欽珮%韓睿明%何禎祥
원성강%흠패%한예명%하정상
海滨锦葵%AFLP%杂种%分子鉴定
海濱錦葵%AFLP%雜種%分子鑒定
해빈금규%AFLP%잡충%분자감정
Kosteletzkya virginica%AFLP%Hybrid%Molecular identification
海滨锦葵是较好的食用和工业利用的盐生经济植物.用AFLP分子标记对海滨锦葵两对杂交亲本的36个F1代个体的真伪进行了鉴定.18个AFLP引物对在亲本Hy103×Hh182间扩增出1100条AFLP带,其中108条是多态带(多态率为9.82%);在亲本Slhy353×Blho301间扩增出1181个AFLP条带,其中148条是多态带(多态率为12.51%).实验结果表明:(1)引物对E+AAC/M+CTC在亲本Hy103× Hh182间扩增出5条父本特征带:401 bp,390 bp,156 bp,137 bp和108bp,引物对E+AAG/M+CTG则产生了7条:457 bp,356 bp,312 bp,251 bp,240 bp,190 bp和76 bp.父本特征带从Hy103×Hh182的8个F1代个体均扩增出,表明其为真杂种;(2)引物对E+AAC/M+CTT在亲本Slhy353×Blho301间扩增出5条父本特征带:440 bp,393 bp,289 bp,162 bp和67 bp,引物对E+ACA/M+CAT则产生了4条:448 bp,360bp,217 bp和145 bp;父本特征带从Slhy353×Blho301 28个F1代个体中的26个个体上扩增出,表明其为真杂种,另外2个为假杂种.
海濱錦葵是較好的食用和工業利用的鹽生經濟植物.用AFLP分子標記對海濱錦葵兩對雜交親本的36箇F1代箇體的真偽進行瞭鑒定.18箇AFLP引物對在親本Hy103×Hh182間擴增齣1100條AFLP帶,其中108條是多態帶(多態率為9.82%);在親本Slhy353×Blho301間擴增齣1181箇AFLP條帶,其中148條是多態帶(多態率為12.51%).實驗結果錶明:(1)引物對E+AAC/M+CTC在親本Hy103× Hh182間擴增齣5條父本特徵帶:401 bp,390 bp,156 bp,137 bp和108bp,引物對E+AAG/M+CTG則產生瞭7條:457 bp,356 bp,312 bp,251 bp,240 bp,190 bp和76 bp.父本特徵帶從Hy103×Hh182的8箇F1代箇體均擴增齣,錶明其為真雜種;(2)引物對E+AAC/M+CTT在親本Slhy353×Blho301間擴增齣5條父本特徵帶:440 bp,393 bp,289 bp,162 bp和67 bp,引物對E+ACA/M+CAT則產生瞭4條:448 bp,360bp,217 bp和145 bp;父本特徵帶從Slhy353×Blho301 28箇F1代箇體中的26箇箇體上擴增齣,錶明其為真雜種,另外2箇為假雜種.
해빈금규시교호적식용화공업이용적염생경제식물.용AFLP분자표기대해빈금규량대잡교친본적36개F1대개체적진위진행료감정.18개AFLP인물대재친본Hy103×Hh182간확증출1100조AFLP대,기중108조시다태대(다태솔위9.82%);재친본Slhy353×Blho301간확증출1181개AFLP조대,기중148조시다태대(다태솔위12.51%).실험결과표명:(1)인물대E+AAC/M+CTC재친본Hy103× Hh182간확증출5조부본특정대:401 bp,390 bp,156 bp,137 bp화108bp,인물대E+AAG/M+CTG칙산생료7조:457 bp,356 bp,312 bp,251 bp,240 bp,190 bp화76 bp.부본특정대종Hy103×Hh182적8개F1대개체균확증출,표명기위진잡충;(2)인물대E+AAC/M+CTT재친본Slhy353×Blho301간확증출5조부본특정대:440 bp,393 bp,289 bp,162 bp화67 bp,인물대E+ACA/M+CAT칙산생료4조:448 bp,360bp,217 bp화145 bp;부본특정대종Slhy353×Blho301 28개F1대개체중적26개개체상확증출,표명기위진잡충,령외2개위가잡충.
Kosteletzkya virginica is a potential halophytic grain crop for consumption and industrial utilization. AFLP markers were used to identify 36 F1 hybrids of two parents. Eighteen peimer combinatios amplified 1100 AFLP band between parent Hy103and Hh182,108(9.82%)of which were polymorphic. Total 1182AFLPP bands were amplified between parent Slhy353 and Blho301 ,148(12.51%) of which were polymorphic. primer combination E+AAC/M+CTC amplified five paternal characteristic bands between Hy103 and Hh182:401bp,390bp,156bp,137bpand 108bp,whereas E+AAC/M+CTT generated seven paternal characteristic bands :457bp,356bp,312bp,251bp,190and 176bp. All these paternal characteristic bands were found in eight F1bhybrids of Hy103×Hh182 ,which were real hybrid .Primer combinatoin E+AAC/M+CTT amplified five paternal characteristic bands between Slhy353 and Blho301:440bp, 393bp,289bp,162bpand 67bp, whereas E+ACA/M+CAT kgenerated four characteristic bands :448bp,360bp,217bpand 145bp.All these paternal characteristic four characteristic bands:448bp,360bp,217bpand 145bp.All these paternal characteristic bands were found in 26of 28F1 hybrids of slhy353×Blho301,and other two F1 hybries were false hybrids.
