生物化学与生物物理进展
生物化學與生物物理進展
생물화학여생물물리진전
PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2007年
2期
176-179
,共4页
王开拓%李兰芬%张晓嫣%梁宇和%魏世成%苏晓东
王開拓%李蘭芬%張曉嫣%樑宇和%魏世成%囌曉東
왕개탁%리란분%장효언%량우화%위세성%소효동
变形链球菌%龋齿%Smu.776%蛋白晶体学
變形鏈毬菌%齲齒%Smu.776%蛋白晶體學
변형련구균%우치%Smu.776%단백정체학
Streptococcus mutans%dental caries%Smu.776%protein crystallography
变形链球菌smu.776基因编码一段含有385个氨基酸的蛋白质,其功能可能为依赖于腺苷甲硫氨酸的甲基转移酶.smu.776的DNA片段被克隆到表达载体pET28a后,在大肠杆菌BL21(DE3)菌株中过量表达得到很好的产量.产物Smu.776蛋白通过Ni2+亲和柱和分子筛柱层析两步得到纯化.采用悬滴气象扩散法得到了Smu.776蛋白的晶体.X射线衍射分辨率达到2.0 (A),晶体属单斜空间群C2,晶格参数为a=168.47 (A),b=50.66 (A),c=53.96 (A),β=104.22°.每个最小不对称单元内含有一个蛋白质分子,溶剂含量为51.3%.
變形鏈毬菌smu.776基因編碼一段含有385箇氨基痠的蛋白質,其功能可能為依賴于腺苷甲硫氨痠的甲基轉移酶.smu.776的DNA片段被剋隆到錶達載體pET28a後,在大腸桿菌BL21(DE3)菌株中過量錶達得到很好的產量.產物Smu.776蛋白通過Ni2+親和柱和分子篩柱層析兩步得到純化.採用懸滴氣象擴散法得到瞭Smu.776蛋白的晶體.X射線衍射分辨率達到2.0 (A),晶體屬單斜空間群C2,晶格參數為a=168.47 (A),b=50.66 (A),c=53.96 (A),β=104.22°.每箇最小不對稱單元內含有一箇蛋白質分子,溶劑含量為51.3%.
변형련구균smu.776기인편마일단함유385개안기산적단백질,기공능가능위의뢰우선감갑류안산적갑기전이매.smu.776적DNA편단피극륭도표체재체pET28a후,재대장간균BL21(DE3)균주중과량표체득도흔호적산량.산물Smu.776단백통과Ni2+친화주화분자사주층석량보득도순화.채용현적기상확산법득도료Smu.776단백적정체.X사선연사분변솔체도2.0 (A),정체속단사공간군C2,정격삼수위a=168.47 (A),b=50.66 (A),c=53.96 (A),β=104.22°.매개최소불대칭단원내함유일개단백질분자,용제함량위51.3%.
The gene smu. 776 encodes a possible S-adenosylmethionine-dependent methyltransferase of 385 residues in Streptococcus mutans, a primary pathogen for human dental caries. The DNA fragment of smu.776 was cloned into pET28a and expressed in good amount from the E. coli strain BL21 (DE3). Smu.776 protein was purified to homogeneity in a two-step procedure ofNi2+ chelating and size exclusion chromatography. Crystals were obtained by hanging-drop vapor diffusion method and diffracted to 2.0 (A) resolution.The crystal belongs to orthorhombic space group C2 with cell dimension of a=168.47 (A), b= 50.66 (A), c=53.96 (A), β=104.22°. The asymmetric unit is expected to contain one molecule with solvent content of 51.3%.