CAJ | 학술논문

目的合成更强抗癌活性的有机锗化合物,研究分子结构和抗癌活性之间的关系.方法首先合成了 4 种含蒽醌酰胺和萘酯基团的新型有机锗化合物,用IR,1HNMR和元素分析研究它们的结构.用MTT 方法研究化合物对人白血病癌细胞(K562)的抑制作用.结果 4种化合物对K562的IC50 分别为11.2,16.3,4.8,9.3μmol/L.而参照物2-氨基蒽醌和1-萘酚的IC50分别为771和282μmol/L,Ge-132对体外培养细胞几乎没有抑制作用.结论对Ge-132进行结构修饰,合成的新型有机锗化合物具有抑制体外培养癌细胞增殖的作用.
목적 합성경강항암활성적유궤타화합물,연구분자결구화항암활성지간적관계.방법 수선합성료 4 충함은곤선알화내지기단적신형유궤타화합물,용IR,1HNMR화원소분석연구타문적결구.용MTT 방법연구화합물대인백혈병암세포(K562)적억제작용.결과 4충화합물대K562적IC50 분별위11.2,16.3,4.8,9.3μmol/L.이삼조물2-안기은곤화1-내분적IC50분별위771화282μmol/L,Ge-132대체외배양세포궤호몰유억제작용.결론 대Ge-132진행결구수식,합성적신형유궤타화합물구유억제체외배양암세포증식적작용.
Objective To synthesize new organogermanium compounds with stronger cytotoxic activities,and study the relationship between the structure and anticancer activity.Methods Four organogermanium compounds with anthraquinone or naphthalene moiety were synthesized,their structures were determined by IR,1HNMR,and elemental analysis.Their cytotoxicity were evaluated against human chronic myeloid leukemia K562 cell lines.The experiments were repeated three times.Results The antiproliferative effect was assayed by determined the IC50,the drug concentration(μmol/L)required to inhibit cell growth by 50%,and the results were 11.2,16.3,4.8,and 9.3,respectively.The antitumor activity was markedly enhanced relative to that of the reference compounds 2-amino-anthraquinone(IC50 =771μmol/L)and 1-naphtol(IC50 =282μmol/L).Conclusion The cytotoxicity could be improved by the introduction of planar aromatic chromophore moiety to the parent compound,Ge-132.