CAJ | 학술논문

建立了用高效液相色谱-二极管阵列检测器(HPLC-DAD)同时检测化妆品中9种水溶性着色剂(溶剂绿7、食品黄3、食品红17、酸性黄1、酸性红33、食品红4、食品红1、橙黄Ⅰ、酸性橙7)的检测方法.不同种类的化妆品采用不同的样品前处理方法提取后,用Diamonsil C18色谱柱分离,以乙腈-磷酸二氢钾缓冲溶液(pH 6)为流动相进行梯度洗脱,检测波长为240 nm,15 min内可对9种目标物同时进行检测,且各化合物都达到基线分离.经测定,该方法的平均回收率(n = 9)为85.33% ～100.2%,相对标准偏差(RSD)为3.68% ～8.20%,检出限为0.01～0.1 mg/L.方法简单、快速,能有效地提取、分离和测定化妆品中9种水溶性着色剂.将该方法用于实际化妆品的检测,结果令人满意.
건립료용고효액상색보-이겁관진렬검측기(HPLC-DAD)동시검측화장품중9충수용성착색제(용제록7、식품황3、식품홍17、산성황1、산성홍33、식품홍4、식품홍1、등황Ⅰ、산성등7)적검측방법.불동충류적화장품채용불동적양품전처리방법제취후,용Diamonsil C18색보주분리,이을정-린산이경갑완충용액(pH 6)위류동상진행제도세탈,검측파장위240 nm,15 min내가대9충목표물동시진행검측,차각화합물도체도기선분리.경측정,해방법적평균회수솔(n = 9)위85.33% ～100.2%,상대표준편차(RSD)위3.68% ～8.20%,검출한위0.01～0.1 mg/L.방법간단、쾌속,능유효지제취、분리화측정화장품중9충수용성착색제.장해방법용우실제화장품적검측,결과령인만의.
An analytical method based on high performance liquid chromatography with diode array detector (HPLC-DAD) has been established for the simultaneous determination of 9 wa-ter-soluble colorants (Solvent Green 7, Food Yellow 3, Food Red 17, Acid Yellow 1, Acid Red 33, Food Red 4, Food Red 1, Orange I , Acid Orange 7) in cosmetics. The different kinds of samples were treated with different preparation methods. The obtained liquid samples were an-alyzed by HPLC using a Diamonsil C18 column (250 mm ×4. 6 mm, 5 μm). Acetonitrile-potas-sium dihydrogen phosphate (KH_2PO_4) buffer solution (pH 6) was used as the mobile phase for gradient elution. The 9 water-soluble colorants were well separated within 15 min. The average recoveries (n =9) were from 85. 33% to 100. 2% with the relative standard deviations (RSDs) between 3. 68% and 8. 20%. The limits of detection (LOD) were in the range of 0. 01 - 0. 1 mg/L. The method is simple, rapid, accurate and suitable for the determination of 9 water-sol-uble colorants in cosmetics.