复旦学报（医学版）
複旦學報（醫學版）
복단학보（의학판）
FUDAN UNIVERSITY JOURNAL OF MEDICAL SCIENCES
2009年
6期
670-674
,共5页
周碧婧%何慧瑾%冯晓源%张善中%杨艳梅
週碧婧%何慧瑾%馮曉源%張善中%楊豔梅
주벽청%하혜근%풍효원%장선중%양염매
胰岛细胞移植%磁共振%超顺磁性氧化铁%活体监测
胰島細胞移植%磁共振%超順磁性氧化鐵%活體鑑測
이도세포이식%자공진%초순자성양화철%활체감측
pancreatic islet cell transplantation%magnetic resonance imaging%superparamagnetic iron oxide%track in vivo
目的 探讨磁共振(magnetic resonance imaging,MRI)对超顺磁性氧化铁(superparamagnetic iron oxide,SPIO)标记的胰岛细胞在大鼠肝脏内的成像方法,用于监测胰岛细胞移植术后移植物在大鼠体内的存活及排异情况.方法 采用GE 3.0T Signa Excite磁共振扫描仪,配合3英寸小动物线圈.实验动物为20只雄性Wistar大鼠和5只雄性Lewis大鼠.将SPIO标记的胰岛细胞移植入大鼠肝脏内,未用SPIO标记的作为阴性对照,比较两组的差异.对SPIO标记后移植的大鼠分别用FSE T2WI序列和GRE T2·WI序列扫描,比较序列敏感性的差异.分别将取自Wistar大鼠和Lewis大鼠的胰岛细胞,用SPIO标记后移植入Wistar大鼠肝脏内,观察两移植组受体体内的胰岛细胞存活及排异的情况.将同基因移植组的大鼠于移植术后3月处死,异基因移植组的大鼠于移植术后3周处死,作病理切片,将所得结果与MRI的图像进行对照.结果 只有SPIO标记后的胰岛细胞可以被MRI监测到,表现为肝实质背景上的低信号点.GRE T2·WI较FSE T2WI序列对SPIO的监测更敏感.同基因移植组于移植后第1周、第2周及第3周的低信号点相对数量分别为(90.03±9.52)%、(92.87±18.21)%和(86.25±24.81)%,而异基因组的相对数量分别为(41.40±15.41)%、(33.41±14.01)%和(23.58±16.78)%.两组相对数量之间的差异具有显著的统计学意义(P<0.01).病理结果显示,标记后的胰岛细胞内确实存在铁颗粒,同基因移植组3月后在肝窦内仍能找到保存完好的移植物,而异基因组在移植后3周只有极少量的移植物残留.结论 MRI能对大鼠体内SPIO标记的胰岛细胞进行成像,用于活体实时监测胰岛细胞移植术后移植物的存活及排异情况.
目的 探討磁共振(magnetic resonance imaging,MRI)對超順磁性氧化鐵(superparamagnetic iron oxide,SPIO)標記的胰島細胞在大鼠肝髒內的成像方法,用于鑑測胰島細胞移植術後移植物在大鼠體內的存活及排異情況.方法 採用GE 3.0T Signa Excite磁共振掃描儀,配閤3英吋小動物線圈.實驗動物為20隻雄性Wistar大鼠和5隻雄性Lewis大鼠.將SPIO標記的胰島細胞移植入大鼠肝髒內,未用SPIO標記的作為陰性對照,比較兩組的差異.對SPIO標記後移植的大鼠分彆用FSE T2WI序列和GRE T2·WI序列掃描,比較序列敏感性的差異.分彆將取自Wistar大鼠和Lewis大鼠的胰島細胞,用SPIO標記後移植入Wistar大鼠肝髒內,觀察兩移植組受體體內的胰島細胞存活及排異的情況.將同基因移植組的大鼠于移植術後3月處死,異基因移植組的大鼠于移植術後3週處死,作病理切片,將所得結果與MRI的圖像進行對照.結果 隻有SPIO標記後的胰島細胞可以被MRI鑑測到,錶現為肝實質揹景上的低信號點.GRE T2·WI較FSE T2WI序列對SPIO的鑑測更敏感.同基因移植組于移植後第1週、第2週及第3週的低信號點相對數量分彆為(90.03±9.52)%、(92.87±18.21)%和(86.25±24.81)%,而異基因組的相對數量分彆為(41.40±15.41)%、(33.41±14.01)%和(23.58±16.78)%.兩組相對數量之間的差異具有顯著的統計學意義(P<0.01).病理結果顯示,標記後的胰島細胞內確實存在鐵顆粒,同基因移植組3月後在肝竇內仍能找到保存完好的移植物,而異基因組在移植後3週隻有極少量的移植物殘留.結論 MRI能對大鼠體內SPIO標記的胰島細胞進行成像,用于活體實時鑑測胰島細胞移植術後移植物的存活及排異情況.
목적 탐토자공진(magnetic resonance imaging,MRI)대초순자성양화철(superparamagnetic iron oxide,SPIO)표기적이도세포재대서간장내적성상방법,용우감측이도세포이식술후이식물재대서체내적존활급배이정황.방법 채용GE 3.0T Signa Excite자공진소묘의,배합3영촌소동물선권.실험동물위20지웅성Wistar대서화5지웅성Lewis대서.장SPIO표기적이도세포이식입대서간장내,미용SPIO표기적작위음성대조,비교량조적차이.대SPIO표기후이식적대서분별용FSE T2WI서렬화GRE T2·WI서렬소묘,비교서렬민감성적차이.분별장취자Wistar대서화Lewis대서적이도세포,용SPIO표기후이식입Wistar대서간장내,관찰량이식조수체체내적이도세포존활급배이적정황.장동기인이식조적대서우이식술후3월처사,이기인이식조적대서우이식술후3주처사,작병리절편,장소득결과여MRI적도상진행대조.결과 지유SPIO표기후적이도세포가이피MRI감측도,표현위간실질배경상적저신호점.GRE T2·WI교FSE T2WI서렬대SPIO적감측경민감.동기인이식조우이식후제1주、제2주급제3주적저신호점상대수량분별위(90.03±9.52)%、(92.87±18.21)%화(86.25±24.81)%,이이기인조적상대수량분별위(41.40±15.41)%、(33.41±14.01)%화(23.58±16.78)%.량조상대수량지간적차이구유현저적통계학의의(P<0.01).병리결과현시,표기후적이도세포내학실존재철과립,동기인이식조3월후재간두내잉능조도보존완호적이식물,이이기인조재이식후3주지유겁소량적이식물잔류.결론 MRI능대대서체내SPIO표기적이도세포진행성상,용우활체실시감측이도세포이식술후이식물적존활급배이정황.
Objective To track superparamagnetic iron oxide (SPIO)-labeled pancreatic islet cells in rats using 3.0T magnetic resonance imaging (MRI), and to detect the survival and rejection of grafts after transplantation. Methods Twenty male Wistar rats and 5 male Lewis rats were included in the study. SPIO-labeled pancreatic islet cells were tracked using a GE 3.0T Signa Excite MRI scanner with an animal coil. The images of SPIO-labeled islet cells in rats after transplantation were compared with those of the unlabeled ones. FSE T2WI sequence and GRE T2*WI sequence were used for the detection. The sensitivity of images for detection of grafts was also compared. SPIO-labeled pancreatic islet cells isolated from Wistar and Lewis rats were transplanted into the liver of Wistar rats. Afterwards, the survival and rejection of islet cells were observed sequentially in these two growps. The rats in the syngeneic group were sacrificed 3 months post-transplantation, while the rats in the allogeneic group were sacrificed 3 weeks post-transplantation. MRI of the grafts were correlated with the pathological results. Results SPIO-labeled pancreatic islet cells were seen on MRI as distinct homogenous, hypointense spots in the liver. GRE T2*WI were more sensitive to the detection of SPIO-labeled islet cells than FSE T2WI. The relative count of hypointense spots in the syngeneic group were (90.03±9.52)%, (92.87±18.21)% and (86.25±24.81)%, respectively at 1 week, 2 weeks and 3 weeks after transplantation, while the relative count in the allogeneic group were (41.40±15.41)%, (33.41±14.01)% and (23.58±16.78)%, respectively. The difference between these counts was statistically significant (P<0.01). Iron particles were detected only in the SPIO-labeled cells. Three months post-transplantation, the grafts were found well-preserved in the liver of the rats of the syngeneic group, while only a few grafts were found in that of the allogeneic group. Conclusions MRI can be used to track SPIO-labeled islet cells in vivo, and has significant value in detecting the survival and rejection of grafts after transplantation in rats.
