中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2010年
8期
1513-1516
,共4页
杨萌%陈民芳%王昊%李长义
楊萌%陳民芳%王昊%李長義
양맹%진민방%왕호%리장의
纳米羟基磷灰石/聚酰胺66%聚酰胺66%生物相容性%细胞培养%骨修复材料
納米羥基燐灰石/聚酰胺66%聚酰胺66%生物相容性%細胞培養%骨脩複材料
납미간기린회석/취선알66%취선알66%생물상용성%세포배양%골수복재료
背景:采用基于纳米羟基磷灰石溶胶新方法制备纳米羟基磷灰石/聚酰胺66复合材料,该材料提高了纳米羟基磷灰石在聚酰胺66基体中的均匀分布和二者的有效键合,进而有利于改善材料的生物性能,有望成为新型骨修复材料.目的:评价纳米羟基磷灰石/聚酰胺66复合材料体内外生物相容性.方法:①将原代培养的成骨细胞与纳米羟基磷灰石/聚酰胺66及聚酰胺66材料复合培养,使用倒置相差显微镜和场发射扫描电子显微镜观察材料周围及表面的细胞形态.②将纳米羟基磷灰石/聚酰胺66复合材料植入兔右侧胫骨,将聚酰胺66作为对照组材料植入兔左侧胫骨.在术后2,8周,取材料周围骨组织进行病理组织切片观察.结果与结论:①纳米羟基磷灰石/聚酰胺66和聚酰胺66未表现出明显的细胞毒性,纳米羟基磷灰石,聚酰胺66材料周围细胞形态好于聚酰胺66,且纳米羟基磷灰石僳酰胺66表面细胞数量多于聚酰胺66,在复合培养的第3天差异尤其显著(P<0.01).②在植入早期,与纳米羟基磷灰石僳酰胺66相接的骨组织成骨细胞活跃且该组材料周围的骨形成过程较对照组更快.结果说明纳米羟基磷灰石/聚酰胺66复合材料较聚酰胺66有更好的生物相容性.
揹景:採用基于納米羥基燐灰石溶膠新方法製備納米羥基燐灰石/聚酰胺66複閤材料,該材料提高瞭納米羥基燐灰石在聚酰胺66基體中的均勻分佈和二者的有效鍵閤,進而有利于改善材料的生物性能,有望成為新型骨脩複材料.目的:評價納米羥基燐灰石/聚酰胺66複閤材料體內外生物相容性.方法:①將原代培養的成骨細胞與納米羥基燐灰石/聚酰胺66及聚酰胺66材料複閤培養,使用倒置相差顯微鏡和場髮射掃描電子顯微鏡觀察材料週圍及錶麵的細胞形態.②將納米羥基燐灰石/聚酰胺66複閤材料植入兔右側脛骨,將聚酰胺66作為對照組材料植入兔左側脛骨.在術後2,8週,取材料週圍骨組織進行病理組織切片觀察.結果與結論:①納米羥基燐灰石/聚酰胺66和聚酰胺66未錶現齣明顯的細胞毒性,納米羥基燐灰石,聚酰胺66材料週圍細胞形態好于聚酰胺66,且納米羥基燐灰石僳酰胺66錶麵細胞數量多于聚酰胺66,在複閤培養的第3天差異尤其顯著(P<0.01).②在植入早期,與納米羥基燐灰石僳酰胺66相接的骨組織成骨細胞活躍且該組材料週圍的骨形成過程較對照組更快.結果說明納米羥基燐灰石/聚酰胺66複閤材料較聚酰胺66有更好的生物相容性.
배경:채용기우납미간기린회석용효신방법제비납미간기린회석/취선알66복합재료,해재료제고료납미간기린회석재취선알66기체중적균균분포화이자적유효건합,진이유리우개선재료적생물성능,유망성위신형골수복재료.목적:평개납미간기린회석/취선알66복합재료체내외생물상용성.방법:①장원대배양적성골세포여납미간기린회석/취선알66급취선알66재료복합배양,사용도치상차현미경화장발사소묘전자현미경관찰재료주위급표면적세포형태.②장납미간기린회석/취선알66복합재료식입토우측경골,장취선알66작위대조조재료식입토좌측경골.재술후2,8주,취재료주위골조직진행병리조직절편관찰.결과여결론:①납미간기린회석/취선알66화취선알66미표현출명현적세포독성,납미간기린회석,취선알66재료주위세포형태호우취선알66,차납미간기린회석속선알66표면세포수량다우취선알66,재복합배양적제3천차이우기현저(P<0.01).②재식입조기,여납미간기린회석속선알66상접적골조직성골세포활약차해조재료주위적골형성과정교대조조경쾌.결과설명납미간기린회석/취선알66복합재료교취선알66유경호적생물상용성.
BACKGROUND: Nanohydroxyapatite reinforced polyamide 66 (n-HA/PA66) prepared using the novel process based on nanohydroxyapatite (n-HA) sol may promote the biocompatibility due to the well distribution of n-HA in the polyamide66 (PA66) matrix and chemical bond at organic-inorganic interface, n-HA/PA66 composite has been proposed as a premising bone repair biomaterial.OBJECTIVE: To investigate the biocompatibility of the novel material both in vitro and in vivo.METHODS: Primarily cultured osteoblasts were co-cultured with n-HA/PA66 and PA66. The cell attachment and morphology were studied using phase contrast light microscope (PCLM) and field-emission scanning electron microscope (FE-SEM).Moreover, n-HA/PA66 pins, with PA66 pins as control, were implanted into the right and left (control group) shinbone shafts of the rabbits, respectively. Materials were harvested at weeks 2, 8 and tissue sections were observed.RESULTS AND CONCLUSION- n-HA/PA66 and PA66 exhibited no cytotoxicity with osteoblasts, while the cellular morphology near the samples was better in the test group than in the control one. Moreover, the cell density attached on n-HA/PA66 was higher than that on PA66 and the cell number were especially different after co-culturing for 3 days (P < 0.01 ). The osteoblasts at the interface between the host bone and n-HA/PA66 also had a higher activity during the earlier period after implantation.Meanwhile the bone formation process was faster and effective in the experimental group. Results suggested that n-HA/PA66 prepared base on the n-HA sol has a better biocompatibility as compared with PA66.