CAJ | 학술논문

目的研究流行于河南省献血员中的人免疫缺陷病毒(HIV)外膜蛋白(env)基因亚型.方法提取HIV感染者外周血单个核白细胞(PBMC)的DNA,经套式-聚合酶链反应(Nested-PCR)扩增env基因片断,对C2-V3区350-450核苷酸顺序进行测定和分析.结果 20份样品的env基因序列与B亚型最为接近,基因离散率为8.34%,与其它国际亚型的基因离散率在20%以上;与流行于云南的B亚型毒株基因离散率为4.18%;样品间的基因离散率为3.4%.结论流行于河南省献血员中的HIV属B亚型,并与云南的流行株密切相关;其在河南的流行时间在3～6年间.
목적 연구류행우하남성헌혈원중적인면역결함병독(HIV)외막단백(env)기인아형.방법 제취HIV감염자외주혈단개핵백세포(PBMC)적DNA,경투식-취합매련반응(Nested-PCR)확증env기인편단,대C2-V3구350-450핵감산순서진행측정화분석.결과 20빈양품적env기인서렬여B아형최위접근,기인리산솔위8.34%,여기타국제아형적기인리산솔재20%이상;여류행우운남적B아형독주기인리산솔위4.18%;양품간적기인리산솔위3.4%.결론 류행우하남성헌혈원중적HIV속B아형,병여운남적류행주밀절상관;기재하남적류행시간재3～6년간.
Objective To identify HIV-1 envelope sequence subtypes in infected blood donors from Henan province.Methods DNA extracted from PBMC were amplified in the presence of env fragment by nested-PCR.The C2-V3 region was sequenced and a phylogenetic tree was constructed.Results The overall env gene divergence is 8.34%between samples with HIV-1 B subtype,and 4.18%with reference strains of Yunnan origin.The sample gene divergence with other subtypes A,C,D,E is over 23%.The innergroup gene divergence of samples is 3.4%.The globally predominant GPGQ sequence at the tip of V3 motif is found in 11 strains(55%),typical European GPGR sequence were found in 7 strains(35%).Two strains contain a GPGK sequence.Conclusion These findings suggest the epidemic of subtype B of HIV-1 in Henan province and it is closely related to those identified in Yunnan province.