CAJ | 학술논문

目的了解缅甸入境健康儿童和云南省口岸地区健康儿童肠道病毒(EV)携带情况及病毒型别特征.方法在10个边境口岸＜15岁的健康儿童中采集粪便标本,进行病毒分离和型别鉴定.共采集到319份粪便标本.脊髓灰质炎(脊灰)病毒(PV)用抗体中和试验鉴定,并送国家脊灰实验室进行型内鉴别;非脊灰肠道病毒(NPEV)用基因测序方法进行鉴定;腺病毒用血清学方法鉴定.结果共检测到EV 53株(带病毒率为16.6%),其中PV23株(阳性率7.2%)经国家脊灰实验室证实均为疫苗株,未发现脊灰野病毒;NPEV 30株(阳性率9.4%),经VP1区核苷酸序列测定,除1株未被所有EV通用引物扩增外,其余29株分别属于HEV-A组(1株,1个血清型,占3.3%)、HEV-B组(20株,11个血清型,占66.7%),HEV-C组(8株,4个血清型,占26.7%),未检测HEV-D组病毒.同时还检测到腺病毒4株,阳性率为1.25%.分别做B组和C组病毒基因树,相同型别的分离株都与对应的标准株聚集在一起.同源性计算表明,分离株与对应标准株的核苷酸同源性及氨基酸同源性分别在75%和85%以上,符合国际测序定型标准.结论在中国云南省与缅甸接壤地区,EV携带率较高,特别是PV阳性率高于急性弛缓性麻痹常规监测.EV中以HEV-B组病毒为主,病毒血清型的分布具有多样性,并发现EV73(2株)、EV75(1株)、EV80(1株)和Ev96(4株)等新型EV.
목적 료해면전입경건강인동화운남성구안지구건강인동장도병독(EV)휴대정황급병독형별특정.방법 재10개변경구안＜15세적건강인동중채집분편표본,진행병독분리화형별감정.공채집도319빈분편표본.척수회질염(척회)병독(PV)용항체중화시험감정,병송국가척회실험실진행형내감별;비척회장도병독(NPEV)용기인측서방법진행감정;선병독용혈청학방법감정.결과 공검측도EV 53주(대병독솔위16.6%),기중PV23주(양성솔7.2%)경국가척회실험실증실균위역묘주,미발현척회야병독;NPEV 30주(양성솔9.4%),경VP1구핵감산서렬측정,제1주미피소유EV통용인물확증외,기여29주분별속우HEV-A조(1주,1개혈청형,점3.3%)、HEV-B조(20주,11개혈청형,점66.7%),HEV-C조(8주,4개혈청형,점26.7%),미검측HEV-D조병독.동시환검측도선병독4주,양성솔위1.25%.분별주B조화C조병독기인수,상동형별적분리주도여대응적표준주취집재일기.동원성계산표명,분리주여대응표준주적핵감산동원성급안기산동원성분별재75%화85%이상,부합국제측서정형표준.결론 재중국운남성여면전접양지구,EV휴대솔교고,특별시PV양성솔고우급성이완성마비상규감측.EV중이HEV-B조병독위주,병독혈청형적분포구유다양성,병발현EV73(2주)、EV75(1주)、EV80(1주)화Ev96(4주)등신형EV.
Objective To explore the enterovirus infection status among healthy children under 15 years old in the border areas of Yunnan province that connecting Myanmar.Methods A total of 319 stool samples were collected from healthy children in the 10 entrance ports.Enterovirus was isolated from these stool samples and then poliovirus and adenovirus were serotyped by neutralization test using specific anti-sera.All the non-polio enteroviruses(NPEVs)were identified by partial sequencing of VP1 gene.Results All 53 enterovirus were isolated from 319 stool samples and 16.6% of them carried the virus.23 polio virus(PVs)and 30 NPEVs were isolated with rates of carrying the virus were 7.2% and 9.4% respectively.4 adenovirus were also isolated with a rate as 1.25%.1 isolate could not be amplified by any Pan-enterovirus primers or by RT-PCR so was not able to be sequenced.The results of NPEVs sequencing showed that:1 isolate(3.3%)was classified into 1 serotype of HEV-A while 20 isolates(66.7%)were classified into 11 serotypes of HEV-B and 8 isolates(26.7%)were classified into 3 serotypes of HEV-C.However,we could not isolate any viruses that belong to HEV-D.nt.Result from the aa identify calculation showed that the nt and aa identification between isolates and corresponding standard strains were more than 75% and 85% respectively.The findings were similar to the international standards.Conclusion Our results showed that the rate of carrying the enterovirus especially poliovirus in some areas of Yunnan province that bordering Myanmar was higher than that of rate through the routine acute flaccid paralysis detection system.Of the enterovirus isolated,HEV-B group appeared the predominant with the wide spread of enterovirus serotype.Some newer enterovirus were also detected such as EV73(2 strains),EV75(1 strain),EV80(1 strain)and EV96(4 strains).