国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
1期
22-24
,共3页
小檗碱%MG63%生长抑制%细胞凋亡
小檗堿%MG63%生長抑製%細胞凋亡
소벽감%MG63%생장억제%세포조망
Berberine%MG63%Growth inhibitory%Cell apoptosis
目的 探讨小檗碱对人成骨肉瘤细胞MG63生存率的影响.方法 将MG63细胞分为不加小檗碱的对照组和加小檗碱的实验组.采用CCK-8法测定各组细胞生存率,采用Western Blot法对小檗碱的作用机制进行探讨.结果 MG63细胞经0(对照组)、1、5、10、20、50 μg/ml浓度的小檗碱处理48 h后,细胞生存率分别为(99.37±1.13)％、(91.37±4.25)％、(72.44±2.89)％、(57.33±1.37)％、(50.14±4.05)％、(37.53±4.14)％.0(对照组)、20、50 μg/ml浓度的小檗碱处理MG63细胞48 h后,Bcl-2/β-action相对半定量表达值分别为(0.4167±0.0115),(0.3458±0.0146),(0.2987±0.0207)；Bax/β-action相对半定量表达值分别为(0.4327±0.0206),(0.5007±0.0089),(0.7115±0.0214)；Caspase-9/β-action相对半定量表达值分别为(0.1459±0.0107),(0.2416±0.0098),(0.2584±0.0330).实验组与对照组比较,差异均有统计学意义(P＜0.05).结论 小檗碱对MG63细胞具有抑制作用,其作用可能是通过调节凋亡基因Bcl-2、Bax的表达,活化caspases-9诱导肿瘤细胞凋亡.
目的 探討小檗堿對人成骨肉瘤細胞MG63生存率的影響.方法 將MG63細胞分為不加小檗堿的對照組和加小檗堿的實驗組.採用CCK-8法測定各組細胞生存率,採用Western Blot法對小檗堿的作用機製進行探討.結果 MG63細胞經0(對照組)、1、5、10、20、50 μg/ml濃度的小檗堿處理48 h後,細胞生存率分彆為(99.37±1.13)％、(91.37±4.25)％、(72.44±2.89)％、(57.33±1.37)％、(50.14±4.05)％、(37.53±4.14)％.0(對照組)、20、50 μg/ml濃度的小檗堿處理MG63細胞48 h後,Bcl-2/β-action相對半定量錶達值分彆為(0.4167±0.0115),(0.3458±0.0146),(0.2987±0.0207)；Bax/β-action相對半定量錶達值分彆為(0.4327±0.0206),(0.5007±0.0089),(0.7115±0.0214)；Caspase-9/β-action相對半定量錶達值分彆為(0.1459±0.0107),(0.2416±0.0098),(0.2584±0.0330).實驗組與對照組比較,差異均有統計學意義(P＜0.05).結論 小檗堿對MG63細胞具有抑製作用,其作用可能是通過調節凋亡基因Bcl-2、Bax的錶達,活化caspases-9誘導腫瘤細胞凋亡.
목적 탐토소벽감대인성골육류세포MG63생존솔적영향.방법 장MG63세포분위불가소벽감적대조조화가소벽감적실험조.채용CCK-8법측정각조세포생존솔,채용Western Blot법대소벽감적작용궤제진행탐토.결과 MG63세포경0(대조조)、1、5、10、20、50 μg/ml농도적소벽감처리48 h후,세포생존솔분별위(99.37±1.13)％、(91.37±4.25)％、(72.44±2.89)％、(57.33±1.37)％、(50.14±4.05)％、(37.53±4.14)％.0(대조조)、20、50 μg/ml농도적소벽감처리MG63세포48 h후,Bcl-2/β-action상대반정량표체치분별위(0.4167±0.0115),(0.3458±0.0146),(0.2987±0.0207)；Bax/β-action상대반정량표체치분별위(0.4327±0.0206),(0.5007±0.0089),(0.7115±0.0214)；Caspase-9/β-action상대반정량표체치분별위(0.1459±0.0107),(0.2416±0.0098),(0.2584±0.0330).실험조여대조조비교,차이균유통계학의의(P＜0.05).결론 소벽감대MG63세포구유억제작용,기작용가능시통과조절조망기인Bcl-2、Bax적표체,활화caspases-9유도종류세포조망.
Objective To discuss the mechanism of the growth inhibitory effects of berberine on human osteosarcoma cell MG63.Methods MG63 cells were divided into a control group and a processing group.The inhibition ratio of two groups was measured by CCK-8 assay,and the mechanism of the growth inhibitory effect was studied by Western Blot assay.Results The inhibition ratio of MG63 cells,which were respectively treated with berberine at 0 (control group),1,5,10,20,50 μg/ml,were (99.37± 1.13) ％,(91.37±4.25)％,(72.44 ± 2.89)％,(57.33 ± 1.37)％,(50.14 ± 4.05)％ and(37.53 ± 4.14)％ after 48h.The semiquantitative expression of Bcl-2 in MG63 treated with berberine at 0 (control group),20,50 μg/ml after 48h was (0.4167±0.0115),(0.3458±0.0146),(0.2987±0.0207),while the Bax and caspase-9 was (0.4327±0.0206),(0.5007±0.0089),(0.7115±0.0214) and (0.1459±0.0107),(0.2416±0.0098),(0.2584±0.0330).Compared with the control group there was statistically significance (P＜0.05).Conclusion Berberine had inhibitory effects of on MG63.The mechanism lied in its regulating the expression of apoptosis gene Bcl-2 and Bax,activating caspases-9,and inducing cell apoptosis.
