CAJ | 학술논문

目的探讨靶向超声分子成像评价肾移植后急性排异反应的可行性.方法采用“亲和素-生物素”桥接法构建携抗细胞间黏附分子-1(ICAM-1)靶向微泡(MBI)和携同型抗体对照微泡(MB).10只SD大鼠行左侧肾异种移植术,术后72 h移植肾随机先后注入MBI和MB(间隔30 min),分别于注入3 min后行移植肾超声造影检查,并测量移植肾声强度(VI),最后进行肾组织病理及免疫组化检测.结果移植肾在注入靶向超声微泡后可见肾区域明显灌注显影,延迟3 min显像MBI组在移植肾可见显著的超声显影增强.而MB组移植肾仅见轻度的超声显影增强,其显影强度较前者明显减弱.MBI组和MB组移植肾VI值分别为(27.0±7.4)U、(10.2±2.4)U,两者之间差异有统计学意义(F=64.744,P＜0.05).结论应用靶向ICAM-1超声微泡和超声造影结合能有效评价大鼠肾移植急性排异.
목적 탐토파향초성분자성상평개신이식후급성배이반응적가행성.방법 채용“친화소-생물소”교접법구건휴항세포간점부분자-1(ICAM-1)파향미포(MBI)화휴동형항체대조미포(MB).10지SD대서행좌측신이충이식술,술후72 h이식신수궤선후주입MBI화MB(간격30 min),분별우주입3 min후행이식신초성조영검사,병측량이식신성강도(VI),최후진행신조직병리급면역조화검측.결과 이식신재주입파향초성미포후가견신구역명현관주현영,연지3 min현상MBI조재이식신가견현저적초성현영증강.이MB조이식신부견경도적초성현영증강,기현영강도교전자명현감약.MBI조화MB조이식신VI치분별위(27.0±7.4)U、(10.2±2.4)U,량자지간차이유통계학의의(F=64.744,P＜0.05).결론응용파향ICAM-1초성미포화초성조영결합능유효평개대서신이식급성배이.
Objective To assess the feasibility of evaluation of renal allograft acute rejection in rat with contrast-enhanced ultrasound ( CEUS ) and targeted microbubbles.Methods Phospholipid microbubbles targeted to intercellular adhesion molecule -1 (ICAM-1)(MBI) and control microbubbles (MB) were created by conjugating monoclonal antibody against ICAM-1 or isotype control antibody to the lipid capsule via “avidin-biotin” bridging.Ten SD rats with acute renal allograft rejection were injected intravenous of MBI and MB in random order with a 30-min interval.After 3 min of intravenous injection of microbubbles,targeted CEUS imaging was performed in all rats.And then the video intensity (VI) was determined.Results In MBI group,a significant ultrasonic enhancement was observed,but it was not very obvious in MB group.Increment in VI value of transplant kidney in MBI group was great and it amounted to (27.0 ± 7.4)U,however,increment in VI value of in MB group was minor and it was merely (10.2 ± 2.4) U,Difference was evident in transplant kidney between of the two groups (P ＜ 0.05).Conclusions Molecular imaging of ICAM-1 with targeted CEUS can evaluate renal allograft acute rejection effectively.